Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,731,531 G→A 6.8% T780M (ACG→ATG) ‡ PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,731,5310GA 6.8% 175.1 / 2.0 59T780M (ACG→ATG) ‡PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base A (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

CCCAGAAGCGCATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGC  >  NC_002947/1731388‑1731678
                                                                                                                                               |                                                                                                                                                   
cccAGAAGCGCATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCg                                                                                                                                                 >  1:212089/1‑148 (MQ=255)
       gcgcATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGGTGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACg                                                                                                                                         <  4:371924/149‑1 (MQ=255)
       gcgcATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCTTTCTTCCGCACGCCGAAGAACg                                                                                                                                         <  7:57833/149‑1 (MQ=255)
         gcATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGc                                                                                                                                       <  2:212089/149‑1 (MQ=255)
             gTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgc                                                                                                                                   >  8:265218/1‑149 (MQ=255)
              tCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgc                                                                                                                                   <  5:242665/148‑1 (MQ=255)
               ccGAACTCGGCAGCCCCTGCACCAGGTAGAAACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgctg                                                                                                                                 >  1:36505/1‑149 (MQ=255)
                  aaCTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGctgctg                                                                                                                              <  2:36505/149‑1 (MQ=255)
                   aCTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTgg                                                                                                                             <  8:140033/149‑1 (MQ=255)
                             cccTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAAc                                                                                                                    >  1:272162/1‑148 (MQ=255)
                                  cACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATa                                                                                                                <  5:145147/147‑1 (MQ=255)
                                              aCCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTgg                                                                                                  >  8:137815/1‑149 (MQ=255)
                                              aCCAAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTgg                                                                                                  >  7:317546/1‑149 (MQ=255)
                                               cccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTgg                                                                                                  <  1:363685/148‑1 (MQ=255)
                                                 cAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAAcc                                                                                                                   >  7:152140/1‑129 (MQ=255)
                                                 cAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAAcc                                                                                                                   <  8:152140/129‑1 (MQ=255)
                                                           ccccACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGa                                                                                     <  2:272162/149‑1 (MQ=255)
                                                                         cATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCgg                                                                       >  7:209383/1‑149 (MQ=255)
                                                                           tgatgaACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGgaagaa                                                                                                                                           <  3:215604/79‑1 (MQ=255)
                                                                           tgatgaACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGgaagaa                                                                                                                                           >  4:215604/1‑79 (MQ=255)
                                                                             atgaACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGCCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGGAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCa                                                                    <  4:379417/148‑1 (MQ=255)
                                                                               gaACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAgcg                                                                 >  4:95151/1‑149 (MQ=255)
                                                                                aaCAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTgaa                                                                                                                         >  3:76636/1‑92 (MQ=255)
                                                                                aaCAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTgaa                                                                                                                         <  4:76636/92‑1 (MQ=255)
                                                                                     ctctTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTg                                                           >  6:79805/1‑149 (MQ=255)
                                                                                         tCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCa                                                                    <  8:334151/136‑1 (MQ=255)
                                                                                         tCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCa                                                                    >  7:334151/1‑136 (MQ=255)
                                                                                            cGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGc                                                    <  2:251710/149‑1 (MQ=255)
                                                                                               gCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCAAGCCCGGCCAGCGCAGCTGCGAAGGCATc                                                 >  4:234402/1‑149 (MQ=255)
                                                                                                 ttCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGc                                                    >  4:89709/1‑144 (MQ=255)
                                                                                                 ttCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGc                                                    <  3:89709/144‑1 (MQ=255)
                                                                                                    ggAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCtt                                              >  3:215753/1‑147 (MQ=255)
                                                                                                    ggAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCtt                                              >  3:220740/1‑147 (MQ=255)
                                                                                                     gAAATCGCCACCAGCAACCCATGGCTGTCCGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAg                                           <  7:284694/149‑1 (MQ=255)
                                                                                                     gAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAAGGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAg                                           <  7:265218/149‑1 (MQ=255)
                                                                                                      aaaTCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAgg                                          <  7:137815/149‑1 (MQ=255)
                                                                                                       aaTCGCCACCAGCAACCCATCGCTGTCCGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGt                                         <  2:162093/149‑1 (MQ=255)
                                                                                                             caccaGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCAccc                                   >  3:122614/1‑149 (MQ=255)
                                                                                                                caGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCa                                >  4:263082/1‑149 (MQ=255)
                                                                                                                caGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCa                                >  6:52155/1‑149 (MQ=255)
                                                                                                                 aGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCAc                               >  1:141008/1‑149 (MQ=255)
                                                                                                                  gCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgctgc                                                                                                                                >  6:236998/1‑51 (MQ=255)
                                                                                                                  gCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgctgc                                                                                                                                <  5:236998/51‑1 (MQ=255)
                                                                                                                   cAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCg                             <  8:209383/149‑1 (MQ=255)
                                                                                                                    aaCCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTTCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCACACCg                             >  8:319186/1‑148 (MQ=255)
                                                                                                                        cATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGTCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTcgg                        >  6:113305/1‑149 (MQ=255)
                                                                                                                            gCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGt                    <  3:234402/149‑1 (MQ=255)
                                                                                                                                tCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACa                 <  4:122614/148‑1 (MQ=255)
                                                                                                                                tCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACa                 <  3:95151/148‑1 (MQ=255)
                                                                                                                                  gccGTTCTTCCGCACGCCGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGa                                            >  6:343927/3‑119 (MQ=255)
                                                                                                                                  gccGTTCTTCCGCACGCCGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGa                                            <  5:343927/117‑1 (MQ=255)
                                                                                                                                   gCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg               >  2:121583/1‑147 (MQ=255)
                                                                                                                                    cGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGccc                                                                         <  7:44380/88‑1 (MQ=255)
                                                                                                                                    cGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGccc                                                                         >  8:44380/1‑88 (MQ=255)
                                                                                                                                     gTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAga           <  5:79805/149‑1 (MQ=255)
                                                                                                                                     gTTCTTCCGCACGCCGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg               >  4:300753/1‑145 (MQ=255)
                                                                                                                                      ttcttcGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAgat          <  5:52155/149‑1 (MQ=255)
                                                                                                                                      ttcttcGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCAGCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAg            >  4:225780/1‑147 (MQ=255)
                                                                                                                                              cGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGc  <  8:317546/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CCCAGAAGCGCATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGC  >  NC_002947/1731388‑1731678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑