| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,797,343 | A→G | 6.8% | K15R (AAG→AGG) ‡ | lpxA → | acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,797,343 | 0 | A | G | 6.8% | 166.2 / 2.5 | 59 | K15R (AAG→AGG) ‡ | lpxA | acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (31/24); new base G (2/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01 | |||||||||||
AGTTCCTCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTC > NC_002947/1797197‑1797487 | aGTTCCTCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGc < 8:280565/149‑1 (MQ=255) tCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCg > 7:308463/1‑149 (MQ=255) aGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGAc > 3:335865/1‑149 (MQ=255) aGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGAc > 4:378881/1‑149 (MQ=255) cGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGc < 2:353965/149‑1 (MQ=255) gTAAAAGCATGCTCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGACT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGACggat < 1:245610/149‑3 (MQ=255) gTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATCTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGc < 1:95922/149‑1 (MQ=255) gTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCg > 5:362736/1‑149 (MQ=255) aGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAg > 5:148257/1‑149 (MQ=255) aGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAg < 7:76211/149‑1 (MQ=255) cTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGccc > 1:71327/1‑149 (MQ=255) tGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCt > 8:192957/1‑149 (MQ=255) gAAGTTCGAGTGCCGCGCCCTTGTCGTTGCCAAGCGGGTCTGCTCGGCATAAATCCCCTGCGCGTAACGCTCCCTATGAAT‑TCTATTGATCCTCGGGCCATTATCTAGCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTg < 2:73434/149‑1 (MQ=255) gAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTg < 3:66662/149‑1 (MQ=255) aaGTTCGAGTGCCGAGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTgg < 1:135861/149‑1 (MQ=255) cGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTc > 1:94212/1‑129 (MQ=255) cGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTc < 2:94212/129‑1 (MQ=255) gAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATc < 3:378881/149‑1 (MQ=255) ccGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTCGTCGATCGTTgg > 8:168039/1‑149 (MQ=255) ccGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATAACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCATTGGTCGATCGATgg > 7:181480/1‑149 (MQ=255) cTTGTCGCTGCCACGCCGGGCTGCCCGCAATAAATCCCCTCGGCGGAAGGCTCCCTATTAAT‑TCGATTGACCCTCGGGCCATACTCCATCCGTCGGCCAAGCGGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGAt < 8:181783/149‑1 (MQ=255) tCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTc > 3:131457/1‑129 (MQ=255) tCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTc < 4:131457/129‑1 (MQ=255) gATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGa > 1:386778/1‑148 (MQ=255) gCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCGTTGGTCGATCGTTGGCCCTGATGTAGAAATCg > 8:323321/1‑149 (MQ=255) aaGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCggg > 1:363538/1‑149 (MQ=255) gCCGGTCTACTCGTCACAAATCACCTGCGCCGACCGCTCCATATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCAGTCGGCCAAGCTGGCCGACGGCGTCGACGTCGTCAATAGGTCGATCGATGGCCCTCAAGTAGAAATCgtg > 2:340339/1‑145 (MQ=255) gCCGGTCGGCTCGGAAGAAATCACCTGCGCGTAACGCTCCCTATTAAT‑TCGATTTATCCTCGGGCCATTATCGATCGGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGa < 4:108039/149‑1 (MQ=255) ggTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAggg > 8:152756/1‑149 (MQ=255) gTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACTGCGTCGAGGTCGGCCCTTTGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAggg > 1:212066/1‑148 (MQ=255) gCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCg > 8:295907/1‑149 (MQ=255) gCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGACg > 1:189924/1‑84 (MQ=39) gCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGACg < 2:189924/84‑1 (MQ=39) aaaTCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCTGGGAGGGCACCGTTATCGGcc > 2:119487/1‑149 (MQ=255) aaTCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGcc < 7:192957/148‑1 (MQ=255) aaTCACCGGCGCGGAACGCGCCCTATCAAT‑CCGATTTATCCTCGGGCCATAATCGATCCGGCGGCCAAGCTGGCCGAGGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAACTCGGGGAGGGCACCGTTATCGGccc < 3:233140/149‑1 (MQ=255) tCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCg > 3:96129/1‑148 (MQ=255) ccTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCAtg > 6:283417/1‑149 (MQ=255) gcgGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTtgtg < 6:148257/149‑1 (MQ=255) gcgGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTtgtg < 6:362736/149‑1 (MQ=255) cgGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGa > 1:123216/1‑111 (MQ=255) ggAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCt > 1:260943/1‑149 (MQ=255) gAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTc < 7:152756/149‑1 (MQ=255) gCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAg < 2:71327/149‑1 (MQ=255) ccTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGc < 7:92145/139‑1 (MQ=255) ccTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGc > 8:92145/1‑139 (MQ=255) tGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGa < 5:283417/147‑1 (MQ=255) aaT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGAccc < 7:295907/148‑1 (MQ=255) aT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCg > 6:52837/1‑148 (MQ=255) tgCCATTATCTATCCGTCGTCCAAGCTGGCCGAGTGCGTCGAGGTCGGCCCTTGGTAGATCGTTGGCCCTTATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAAcc < 2:212066/148‑1 (MQ=255) cATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCTCCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACATCCGTa > 3:318919/1‑149 (MQ=255) tCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCttt > 5:253054/1‑149 (MQ=255) gATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAAACGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCCTCGGCAAGCACACCCCTATcggt > 4:242116/1‑144 (MQ=255) cGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGttt > 1:346728/1‑149 (MQ=255) cGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGtt < 7:168039/148‑1 (MQ=255) gTCGGCCAGCTTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGtttt > 2:344143/1‑149 (MQ=255) gCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTtgtg < 3:279612/96‑1 (MQ=255) gCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTtgtg > 4:279612/1‑96 (MQ=255) ccAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTc > 6:188521/1‑148 (MQ=255) | AGTTCCTCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAAT‑TCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTC > NC_002947/1797197‑1797487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |