Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,859,114	A→T	6.9%	intergenic (+34/+67)	PP_1661 → / ← PP_1662	dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,859,114	0	A	T	6.9%	197.5 / 3.2	67	intergenic (+34/+67)	PP_1661/PP_1662	dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base T (3/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCC  >  NC_002947/1858967‑1859262                                                                                                                                                    |                                                                                                                                                       cAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAAt                                                                                                                                                       >  6:279680/1‑149 (MQ=255)  aGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATg                                                                                                                                                      >  4:307494/1‑149 (MQ=255)        gTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCg‑cag                                                                                                                                              >  7:257228/1‑149 (MQ=255)          gAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCg‑cagca                                                                                                                                            >  3:233230/1‑149 (MQ=255)             ttGCAGCTGTGCATCAATGACGACCTGGACGGGCTTTAGGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCAttt                                                                                                                                         <  1:146169/149‑1 (MQ=255)             ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCAttt                                                                                                                                         >  5:259416/1‑149 (MQ=255)                 aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTggg                                                                                                                                     >  5:112904/1‑149 (MQ=255)                 aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTggg                                                                                                                                     <  5:24068/149‑1 (MQ=255)                 aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTggg                                                                                                                                     >  5:114389/1‑149 (MQ=255)                   cTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGt                                                                                                                                   <  5:217783/149‑1 (MQ=255)                         aTCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCa                                                                                                                             <  6:263892/149‑1 (MQ=255)                            aaTGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACt                                                                                                                           >  3:307673/1‑148 (MQ=255)                                acgacCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGc                                                                                                                       <  3:307494/148‑1 (MQ=255)                                 cgacCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGcc                                                                                                                      >  4:126249/1‑148 (MQ=255)                                           gggCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGt                                                                                                             >  8:144057/1‑147 (MQ=255)                                            ggCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAg                                                                                                          <  7:144057/149‑1 (MQ=255)                                             gCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGGGTCAgg                                                                                                         <  6:121709/149‑1 (MQ=255)                                             gCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCACGCGAGTCAgg                                                                                                         <  6:26474/149‑1 (MQ=255)                                                ttACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCg‑c                                                                                                                                                <  2:309783/107‑1 (MQ=255)                                                ttACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCg‑c                                                                                                                                                >  1:309783/1‑107 (MQ=255)                                                    ggggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgc                                                                                                  <  3:126249/149‑1 (MQ=255)                                                          ggCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAAt                                                                                            <  6:259416/149‑1 (MQ=255)                                                               gAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGc                                                                                        >  5:318228/1‑148 (MQ=255)                                                                      aaCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCAt                                                                                <  5:13893/149‑1 (MQ=255)                                                                       aCCTGGGCAGCCTGTCGGTAGATGTGCGTTTCGGGGGGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCATTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCAtt                                                                               <  1:81231/149‑1 (MQ=255)                                                                       aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CAG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCAt                                                                                <  7:163128/148‑1 (MQ=255)                                                                            ggCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtgtg                                                                          <  2:61414/149‑1 (MQ=255)                                                                            ggCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtgt                                                                           <  8:257228/148‑1 (MQ=255)                                                                            ggCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtgt                                                                           <  4:307673/148‑1 (MQ=255)                                                                             gCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTgg                                                                         >  4:373513/1‑149 (MQ=255)                                                                                 ccTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAgg                                                                                                                  <  7:158367/104‑1 (MQ=255)                                                                                 ccTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAgg                                                                                                                  >  8:158367/1‑104 (MQ=255)                                                                                 ccTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGgcgcg                                                                     >  2:319443/1‑149 (MQ=255)                                                                                   tGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGTGCAGGGTCATTGTGTGGCGCGCt                                                                   >  5:393376/1‑149 (MQ=255)                                                                                     tCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTg                                                                  <  5:61863/148‑1 (MQ=255)                                                                                       ggTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgg                                                                                                         >  6:99164/1‑107 (MQ=255)                                                                                       ggTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgg                                                                                                         <  5:99164/107‑1 (MQ=255)                                                                                        gTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGTGTCAGGGGTTCGCGGCAATGGGCAGGGTGATTGTGTGGCGCGCTGGttt                                                              >  5:393021/1‑149 (MQ=255)                                                                                        gTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGttt                                                              >  6:152520/1‑149 (MQ=255)                                                                                             ggTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCCGACAGAATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGAAGGGTCATTGGTTGGCGCGCTGGTTTTc                                                            >  1:111608/1‑148 (MQ=255)                                                                                              gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGc                                                                                        >  7:314244/1‑117 (MQ=255)                                                                                              gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGc                                                                                        <  8:314244/117‑1 (MQ=255)                                                                                                       ggggCTGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGTGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAg                                                <  5:110611/148‑1 (MQ=255)                                                                                                          gCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTc                                             >  1:267259/1‑148 (MQ=255)                                                                                                               gtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGCGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAAcgcg                                       <  4:233230/149‑1 (MQ=255)                                                                                                                   gCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGTGCAGGGTCATTGTGTGGCGCGCTGGTTTTACAGGTCTTCAAGCTCAACGCGGTAt                                   >  1:219649/1‑149 (MQ=255)                                                                                                                     ccTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                                 >  3:312966/1‑149 (MQ=255)                                                                                                                           aTGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGc                           >  7:214959/1‑149 (MQ=255)                                                                                                                            gagCAACCCCAAAATGCTGCGGCATTG‑CCG‑GAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCt                          >  2:306925/3‑149 (MQ=37)                                                                                                                              gCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                        >  7:329802/1‑149 (MQ=255)                                                                                                                                 aCCCCAAAATGCTGCGGCATTG‑CCG‑GAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgtg                     >  3:30367/1‑149 (MQ=38)                                                                                                                                  ccccAAAATGCTGCGGCATTG‑CCG‑GAGCATCGTGGGGTTGC‑CACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                   >  3:174396/1‑149 (MQ=38)                                                                                                                                   cccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                        >  3:379647/1‑144 (MQ=255)                                                                                                                                   cccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                        <  4:379647/144‑1 (MQ=255)                                                                                                                                   cccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                   <  3:373513/149‑1 (MQ=255)                                                                                                                                   cccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                   >  4:382154/1‑149 (MQ=255)                                                                                                                                   cccACGATGCTCAGGCAATG‑CAG‑CAGCATTTTGGGGTTGCTCACTGAGACCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                   >  2:220603/1‑149 (MQ=255)                                                                                                                                    ccaaaaTGCTGCGGCATTG‑CCG‑GAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                  <  1:306925/144‑1 (MQ=38)                                                                                                                                    ccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                  <  8:329802/149‑1 (MQ=255)                                                                                                                                    ccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                  <  6:114389/149‑1 (MQ=255)                                                                                                                                    ccACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                  <  6:112904/149‑1 (MQ=255)                                                                                                                                     cACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg                 >  5:176776/1‑149 (MQ=255)                                                                                                                                     cACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg                 >  4:344172/1‑149 (MQ=255)                                                                                                                                        aaTGCTGCGGCATTG‑CCG‑GAGCATCGTGGGGTTGC‑CACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTAt             <  4:174396/148‑1 (MQ=38)                                                                                                                                           gCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATg            >  2:308486/1‑148 (MQ=255)                                                                                                                                             tCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGaa         >  1:188469/1‑149 (MQ=255)                                                                                                                                                ggCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACAt      <  6:176776/149‑1 (MQ=255)                                                                                                                                                    aTG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGccc  <  3:344172/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CAGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATG‑CCG‑CAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCC  >  NC_002947/1858967‑1859262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑