Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,892,529 C→G 7.9% intergenic (+39/‑56) PP_1697 → / → PP_1698 transcriptional regulator, GntR family/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,892,5290CG 7.9% 238.5 / 8.8 76intergenic (+39/‑56)PP_1697/PP_1698transcriptional regulator, GntR family/MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (31/39);  new base G (3/3);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01

GGTGGTCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGC  >  NC_002947/1892382‑1892675
                                                                                                                                                   |                                                                                                                                                  
ggtggtCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGcc                                                                                                                                                   <  5:295057/149‑1 (MQ=255)
  tggtCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCAc                                                                                                                                                 >  6:241738/1‑149 (MQ=255)
     tCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGgc                                                                                                                                              >  6:198109/1‑149 (MQ=255)
       gTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGgcgc                                                                                                                                            <  2:112396/149‑1 (MQ=255)
       gTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGgcg                                                                                                                                             >  5:40496/1‑148 (MQ=255)
        tGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGGCGCa                                                                                                                                           <  5:249969/149‑1 (MQ=255)
        tGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGATTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGgcg                                                                                                                                             >  8:84300/1‑147 (MQ=255)
         gATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGgcgc                                                                                                                                            >  1:33653/1‑147 (MQ=255)
         gATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGgcgc                                                                                                                                            <  2:33653/147‑1 (MQ=255)
           tCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACTGCGCAGTg                                                                                                                                        <  2:204668/149‑1 (MQ=255)
                cgcCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTc                                                                                                                                   <  2:305226/149‑1 (MQ=255)
                  ccTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGc                                                                                                                                 >  5:64011/1‑149 (MQ=255)
                        ccGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGccc                                                                                                                           <  7:202401/149‑1 (MQ=255)
                                aCGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCacac                                                                                                                    <  3:201405/148‑1 (MQ=255)
                                aCGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACAcc                                                                                                                   >  5:232248/1‑149 (MQ=255)
                                 cGCCGGCAAACCTCTGGAATGGCGCCTCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACAcc                                                                                                                   <  1:145881/148‑1 (MQ=255)
                                  gCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCa                                                                                                                 >  4:173511/1‑149 (MQ=255)
                                  gCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTCCCCGTCACACCCa                                                                                                                 >  4:250607/1‑149 (MQ=255)
                                   ccGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCAc                                                                                                                <  7:178411/149‑1 (MQ=255)
                                     ggCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGa                                                                                                              <  6:232248/149‑1 (MQ=255)
                                     ggCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGa                                                                                                              <  5:57203/149‑1 (MQ=255)
                                        aaaCCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACAcc                                                                                                                   >  1:71363/1‑141 (MQ=255)
                                        aaaCCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACAcc                                                                                                                   <  2:71363/141‑1 (MQ=255)
                                         aaCCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGAc                                                                                                             >  4:145071/1‑146 (MQ=255)
                                          aCCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAg                                                                                                          >  1:103903/1‑148 (MQ=255)
                                           ccTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAg                                                                                                          >  3:177139/1‑147 (MQ=255)
                                           ccTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCACATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGaa                                                                                                        <  7:173649/149‑1 (MQ=255)
                                             tctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAg                                                                                                       >  2:233731/1‑148 (MQ=255)
                                              ctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGa                                                                                                     >  3:236511/1‑149 (MQ=255)
                                               tGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCTCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGAAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAgg                                                                                                      <  7:169217/147‑1 (MQ=255)
                                               tGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAgg                                                                                                      <  7:164237/147‑1 (MQ=255)
                                               tGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAgg                                                                                                      <  3:60601/147‑1 (MQ=255)
                                                 gAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGAt                                                                                                    <  5:198109/147‑1 (MQ=255)
                                                 gAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGAt                                                                                                    <  4:236511/147‑1 (MQ=255)
                                                 gAATGGCGCCGCGCGCGCGGCCATGCGCAGCACTACCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTCCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATaa                                                                                                  <  8:136606/149‑1 (MQ=255)
                                                  aaTGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAAc                                                                                                 <  8:346018/149‑1 (MQ=255)
                                                     ggCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACGGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACaa                                                                                               >  1:112864/1‑148 (MQ=255)
                                                      gcgcCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATc                                                                                             <  6:40496/149‑1 (MQ=255)
                                                      gcgcCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATc                                                                                             <  3:244974/149‑1 (MQ=255)
                                                       cgccgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCa                                                                                            >  6:36010/1‑149 (MQ=255)
                                                                cgcgGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCt                                                                                   <  6:64011/149‑1 (MQ=255)
                                                                   ggCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGt                                                                                <  1:12007/149‑1 (MQ=255)
                                                                          cgcAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgcg                                                                          <  5:241738/148‑1 (MQ=255)
                                                                          cgcAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCga                                                                         <  3:145071/149‑1 (MQ=255)
                                                                           gcagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGt                                                                                <  8:246445/141‑1 (MQ=255)
                                                                           gcagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgc                                                                           >  8:252751/1‑146 (MQ=255)
                                                                           gcagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGAGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGt                                                                                >  7:246445/1‑141 (MQ=255)
                                                                             agcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGAt                                                                      <  1:371802/149‑1 (MQ=255)
                                                                                   tCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCt                                                                 >  7:307326/1‑148 (MQ=255)
                                                                                      gCTACTGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgc                                                                           >  4:326958/1‑135 (MQ=255)
                                                                                      gCTACTGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgc                                                                           <  3:326958/135‑1 (MQ=255)
                                                                                       cTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACgg                                                            <  7:252751/149‑1 (MQ=255)
                                                                                           cGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAAc                                                        <  3:250607/149‑1 (MQ=255)
                                                                                            gCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACg                                                       <  3:173511/149‑1 (MQ=255)
                                                                                                        gTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTg                                           <  1:15768/149‑1 (MQ=255)
                                                                                                               cGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgcg                                                                          <  1:338516/111‑1 (MQ=255)
                                                                                                               cGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgcg                                                                          >  2:338516/1‑111 (MQ=255)
                                                                                                               cGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGt                                    >  7:357573/1‑149 (MQ=255)
                                                                                                                      ttcacccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcggcg                             >  5:23730/1‑149 (MQ=255)
                                                                                                                      ttcacccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcggag                             >  7:65129/1‑147 (MQ=255)
                                                                                                                      ttcacccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCATCGCCTGCCCGTAACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGATATCACCTCACGGGAACGCAAGACATTCAGGCCCTGTTTcggcg                             >  4:146000/1‑149 (MQ=255)
                                                                                                                        cacccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcggcgg                            <  5:164837/148‑1 (MQ=255)
                                                                                                                        cacccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcggcgg                            <  3:307720/148‑1 (MQ=255)
                                                                                                                           ccttcaccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTgg                        >  5:26831/1‑149 (MQ=255)
                                                                                                                               caccTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTc                      <  2:103903/147‑1 (MQ=255)
                                                                                                                                accTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTgg                                          <  6:9128/126‑1 (MQ=255)
                                                                                                                                accTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTgg                                          >  5:9128/1‑126 (MQ=255)
                                                                                                                                  cTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTc                  >  8:111557/1‑148 (MQ=255)
                                                                                                                                  cTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTc                  >  1:172547/1‑148 (MQ=255)
                                                                                                                                    cacTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGAt               >  3:186099/3‑149 (MQ=255)
                                                                                                                                     tCTGCGCCGTTCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGAt               <  3:146000/148‑1 (MQ=255)
                                                                                                                                     tCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGAt               <  8:43196/148‑1 (MQ=255)
                                                                                                                                     tCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATg              >  4:165879/1‑149 (MQ=255)
                                                                                                                                     acTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATg              >  5:303335/2‑149 (MQ=255)
                                                                                                                                         cgcCGTGCTGCCACGGCGCAGTGGCTTAGCATGCCCGTCACACCCACGACTAGAATGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGTGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATgcgc           <  7:84300/148‑1 (MQ=255)
                                                                                                                                           ccGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTg         >  2:122076/1‑148 (MQ=255)
                                                                                                                                             gcgaagcCACTGCGCCGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTgg        >  6:182961/6‑147 (MQ=255)
                                                                                                                                             gTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGaa      <  8:357573/149‑1 (MQ=255)
                                                                                                                                              cgaagcCACTGCGCCGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAg     <  5:182961/145‑1 (MQ=255)
                                                                                                                                                ctgcCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGACAACAATCATGTTCAGCTGGTACCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTg   <  4:177139/149‑1 (MQ=255)
                                                                                                                                                   ccACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGc  <  5:36010/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTGGTCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGC  >  NC_002947/1892382‑1892675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑