| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,905,820 | A→C | 8.2% | E692A (GAG→GCG) ‡ | nirB → | nitrite reductase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,905,820 | 0 | A | C | 8.2% | 181.1 / 7.7 | 61 | E692A (GAG→GCG) ‡ | nirB | nitrite reductase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (26/30); new base C (3/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01 | |||||||||||
TAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGACC > NC_002947/1905673‑1905963 | tAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAg < 3:105156/149‑1 (MQ=255) aaaGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTGTCGGGATGCACCCGTGAATGCGCCGAgg > 1:93926/1‑149 (MQ=255) cgtgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCTCGGCGCattcac < 4:113028/149‑6 (MQ=255) gtgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCTCGGCGCattcacg < 4:51316/149‑7 (MQ=255) ggCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGa > 7:257607/1‑149 (MQ=255) agcaCCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCCCCCGTGAATGCGCCGAGGCGCAGAGCAAGGACAt > 4:268583/1‑149 (MQ=255) ccTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGc < 8:257607/149‑1 (MQ=255) ttACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCCTGCGCAGCCCCCACAAGCTCAAGTTCGGGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGCCATCGGCGTTATCGc > 1:296664/1‑148 (MQ=255) atggCGTGCAGGACAGGGTGGCCATGTGCCTGCGCCGGGAAGCTCGCGACAAGGG‑CCTGCGCAGCCCCCACACGCTCAAGTGCGCGGTAGCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCACGGACATCGGCGTTATCGCCAc < 2:136183/146‑1 (MQ=255) aCGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCAc < 4:89093/148‑1 (MQ=255) cGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCg < 5:335289/149‑1 (MQ=255) tGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAg < 4:40151/149‑1 (MQ=255) cAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAgg < 8:79071/148‑1 (MQ=255) gtgtCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAAc > 4:353780/1‑149 (MQ=255) gtgtCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAAc < 3:350281/149‑1 (MQ=255) gtgtCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAAc < 4:101074/149‑1 (MQ=255) gtggCGCCATGGCCCTGCGCCTGGAAGATCGCTACACGGG‑CCTGCGCAGCCCCCACCAGCTCAAGTTCGCGGTATCGGGATGCACCCTTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAAc < 8:311928/149‑1 (MQ=255) tgtCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATc < 6:119857/127‑1 (MQ=255) tgtCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAAcc < 2:157354/149‑1 (MQ=255) tCGCCATGGCCCTGCTCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTAATGTTCGCGGTATGTGGATGCACCCGTGAATGCGCCGAGGCGCAGAGAAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTg < 3:305531/149‑1 (MQ=255) ccATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTAc < 7:362990/149‑1 (MQ=255) gCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGgcgc > 2:163794/1‑94 (MQ=255) gCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGgcgc < 1:163794/94‑1 (MQ=255) ccTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCg < 7:51715/149‑1 (MQ=255) ccTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCg < 1:227996/149‑1 (MQ=255) gcCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTGTCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAAc < 2:93926/149‑1 (MQ=255) tGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAAcggc > 1:188924/1‑149 (MQ=255) ggAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAAcggcg > 2:234821/1‑149 (MQ=255) aTCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATg > 2:197708/1‑149 (MQ=255) tCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATgc > 5:298023/1‑149 (MQ=255) tACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGccc > 3:289668/1‑149 (MQ=255) cAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATgcg > 6:24028/1‑144 (MQ=255) cAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATgcg < 5:24028/144‑1 (MQ=255) cTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGc > 7:235603/1‑149 (MQ=255) cgcAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCCGCGGGCGCATGCCgc < 6:34699/149‑2 (MQ=255) cccACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTc < 2:82245/149‑1 (MQ=255) ccACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATc > 6:322253/1‑76 (MQ=255) ccACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATc < 5:322253/76‑1 (MQ=255) ccACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCg > 8:89714/1‑149 (MQ=255) cacaAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGc > 4:367117/1‑149 (MQ=255) aaGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCAc < 3:268583/149‑1 (MQ=255) aaGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCAc > 2:89997/1‑149 (MQ=255) acgTTCGCGGTATCGGGATGCACCCGTTAATGCGCCGAGGCGCAGAGCAAGTACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCG‑CCGCGGCATGCCGAGCTGTTCGCCACCGACCTg < 3:328187/147‑1 (MQ=255) ttCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGga < 6:298023/149‑1 (MQ=255) cgGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGgacgac < 4:289668/149‑1 (MQ=255) gggATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGccc > 4:354819/1‑149 (MQ=255) ggATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCt > 6:16036/1‑149 (MQ=255) ggATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCt > 8:145383/1‑149 (MQ=255) aCCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACg < 7:145383/149‑1 (MQ=255) cccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCt < 4:60294/127‑1 (MQ=255) cccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCt > 3:60294/1‑127 (MQ=255) cccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGc > 5:15318/1‑149 (MQ=255) ccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGcc > 5:208184/1‑149 (MQ=255) ttgctctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCt > 6:301416/7‑149 (MQ=255) ttgctctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCt > 1:168211/7‑149 (MQ=255) tgctctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTAGTACGCCt > 5:115297/6‑148 (MQ=255) gTGAATGCGCCGAGGCGCAGAGCAAGGACATAGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCTTGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACACCt > 5:355738/1‑148 (MQ=255) gAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGAt > 6:344006/1‑149 (MQ=255) cgcCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGAcc < 2:188924/149‑1 (MQ=255) gcCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGaa > 8:147575/1‑126 (MQ=255) gcCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGaa < 7:147575/126‑1 (MQ=255) | TAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGG‑CCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGAACCTGTACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGACC > NC_002947/1905673‑1905963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |