Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,914,295	A→G	7.3%	Q221R (CAG→CGG) ‡	pcaQ →	transcriptional regulator PcaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,914,295	0	A	G	7.3%	195.9 / 3.1	67	Q221R (CAG→CGG) ‡	pcaQ	transcriptional regulator PcaQ
Reads supporting (aligned to +/- strand):  ref base A (26/36);  new base G (2/3);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGTGGTGCGCCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTGGGTGTGCGT  >  NC_002947/1914152‑1914443                                                                                                                                                |                                                                                                                                                       ccTGGTGGTGCGCCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGc                                                                                                                                                   >  5:284570/1‑149 (MQ=255)          gcgcCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCTGGATGCCgca                                                                                                                                               >  4:245620/1‑142 (MQ=255)          gcgcCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCTGGATGCCgca                                                                                                                                               <  3:245620/144‑3 (MQ=255)           cgcCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCTGGATGCCgcattgca                                                                                                                                          <  2:98426/148‑8 (MQ=255)           cgcCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAgc                                                                                                                                          <  3:188569/148‑1 (MQ=255)            gcCCGGTTCACCCGCTGATGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTCTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAgcgc                                                                                                                                        <  6:77495/149‑1 (MQ=255)              ccGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCt                                                                                                                                      <  7:316011/149‑1 (MQ=255)               cGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTg                                                                                                                                     <  5:318147/149‑1 (MQ=255)                gggTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTgg                                                                                                                                    <  5:389953/149‑1 (MQ=255)                      cccGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑Acccc                                                                                                                            >  8:136725/1‑149 (MQ=255)                      cccGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AAccc                                                                                                                            <  6:158350/149‑1 (MQ=255)                       ccGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCt                                                                                                                           >  8:93793/1‑149 (MQ=255)                                   aGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCt                                                                                                               >  5:45072/1‑149 (MQ=255)                                     cACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCt                                                                                                               >  3:120753/1‑147 (MQ=255)                                     cACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGa                                                                                                             <  6:45072/149‑1 (MQ=255)                                          ccGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCgg                                                                                                        >  7:287912/1‑149 (MQ=255)                                           cGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGgc                                                                                                       >  8:133144/1‑149 (MQ=255)                                            gTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGgcg                                                                                                      >  3:198727/1‑149 (MQ=255)                                            gTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGgcg                                                                                                      <  4:367697/149‑1 (MQ=255)                                              ggAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGgcgc                                                                                                     >  6:47145/1‑148 (MQ=255)                                                  cGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACct                                                                                                >  2:199310/1‑149 (MQ=255)                                                  cGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACct                                                                                                <  6:19025/149‑1 (MQ=255)                                                   gAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACctg                                                                                               <  7:93793/149‑1 (MQ=255)                                                   gAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCCGGCTCAGGGCCAgcgacagggt                                                                                               <  5:218121/149‑8 (MQ=255)                                                      gACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑ATCCCTGTCGCTGGCCCTGAGCCGGCGCTACctgctg                                                                                            <  5:43287/149‑1 (MQ=255)                                                       aCAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCCTGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTgg                                                                                           <  2:132315/149‑1 (MQ=255)                                                       aCAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTgg                                                                                           <  6:385043/149‑1 (MQ=255)                                                       aCAGGTGGGGCGTTACCCGCTTGGGCTGCGGCCGGCCGGCACCACCATCCGTCAGCATGCCGACTGCCTGTGTGGGCAATGCGGCAGCCAGATGGGGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTgg                                                                                           <  6:379901/149‑1 (MQ=255)                                                        cAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTggg                                                                                          <  5:196815/149‑1 (MQ=255)                                                                   ttACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGc                                                                                                           <  8:207554/121‑1 (MQ=255)                                                                   ttACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGc                                                                                                           >  7:207554/1‑121 (MQ=255)                                                                       ccGCTTGTGCTGCCGCCGGCCGGCACCACCTTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCtgtg                                                                           >  1:32654/1‑149 (MQ=255)                                                                          cTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTgtgggt                                                                        <  7:38081/149‑1 (MQ=255)                                                                          atggtggtgCCGGCCGGCGGCAGCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTgtgggt                                                                        >  8:130514/4‑149 (MQ=255)                                                                              tgctgcCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGgcg                                                                    <  6:284570/149‑1 (MQ=255)                                                                                 tgcCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGcgccg                                                                 >  6:275/1‑149 (MQ=255)                                                                                  gccgccGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGcgccgc                                                                >  5:242425/1‑149 (MQ=255)                                                                                   cggccgg‑cggCAGCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGt                                                              <  7:130514/147‑1 (MQ=255)                                                                                   ccgccgGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCcgcg                                                               >  2:102964/1‑149 (MQ=255)                                                                                     gccggccgGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTgg                                                                                           <  3:146709/119‑1 (MQ=255)                                                                                     gccggccgGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCACTGAGCCGGCGCTACCTGCTgg                                                                                           >  4:146709/1‑119 (MQ=255)                                                                                          ccggcACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGcc                                                        <  4:120753/149‑1 (MQ=255)                                                                                             gcACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGAGGAACCCTGTCGCTGGCCCTCAGCCCCCGCTAAATGATGTGTATCGCTGTCCTGTGGGTGGCGCCGCGAGATGACg                                                       >  1:121150/1‑149 (MQ=255)                                                                                                ccaccaTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGtgctg                                                  <  1:199310/149‑1 (MQ=255)                                                                                                 caccaTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGcgccgc                                                                >  1:261174/1‑134 (MQ=255)                                                                                                 caccaTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGcgccgc                                                                <  2:261174/134‑1 (MQ=255)                                                                                                   ccaTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTc                                               <  7:133144/149‑1 (MQ=255)                                                                                                   ccaTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTc                                               >  3:187641/1‑149 (MQ=255)                                                                                                     aTCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGa                                             >  7:285638/1‑149 (MQ=255)                                                                                                      tCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGAc                                            <  3:350421/149‑1 (MQ=255)                                                                                                      tCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGAc                                            <  7:65111/149‑1 (MQ=255)                                                                                                               aTGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGt                                   <  2:281782/149‑1 (MQ=255)                                                                                                                tGCCGTCAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGTA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTg                                  <  4:124989/149‑1 (MQ=255)                                                                                                                  ccGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGc                                <  8:287912/149‑1 (MQ=255)                                                                                                                   cGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGc                                >  1:370734/1‑148 (MQ=255)                                                                                                                    gACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCg                               >  8:7277/1‑148 (MQ=255)                                                                                                                     aCAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGaa                             <  4:198727/149‑1 (MQ=255)                                                                                                                      cAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAAc                            >  5:77858/1‑149 (MQ=255)                                                                                                                             tttGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTc                       <  1:102964/147‑1 (MQ=255)                                                                                                                             tttGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTc                       >  4:153284/1‑147 (MQ=255)                                                                                                                             tttGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCg                      <  2:370734/148‑1 (MQ=255)                                                                                                                                 tgCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCtt                 >  3:263032/1‑149 (MQ=255)                                                                                                                                       ccGGCATCTGGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGa                     >  2:32565/2‑139 (MQ=255)                                                                                                                                       ccGGCATCTGGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCAGCTCGACCTGCGCCGTGGCGAACTGGTCGa                     <  1:32565/138‑1 (MQ=255)                                                                                                                                        cGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGCTGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTgg          <  2:121150/149‑1 (MQ=255)                                                                                                                                         ggCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTggg         <  4:281846/149‑1 (MQ=255)                                                                                                                                               cAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTGGGTGTGCg   <  8:285638/149‑1 (MQ=255)                                                                                                                                                aGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTGGGTGTGCGt  >  5:318411/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                       CCTGGTGGTGCGCCCGGGTCACCCGCTGCTGGCCAGCACGCCCGTGGAGCGAGGACAGGTGGGGCGTTACCCGCTTGTGCTGCCGCCGGCCGGCACCACCATCCGTCAGCATGCCGACAGCCTGTTTGTGCAATGCGGCATCCAGATGCCGGCCCAGCGCCTGGA‑‑AACCCTGTCGCTGGCCCTGAGCCGGCGCTACCTGCTGGGTAGCGATGGCCTGTGGGTGGCGCCGCGTGATGCCGTGCTGCTCGACCTGCGCCGTGGCGAACTGGTCGAGCTTGACCTGGGTGTGCGT  >  NC_002947/1914152‑1914443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑