Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,937,201 A→G 6.6% D161D (GAT→GAC)  PP_1737 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,937,2010AG 6.6% 174.3 / 2.2 60D161D (GAT→GAC) PP_1737hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base G (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GCAGGGCGAAGATTTCCGGGTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGCCGAGCGCTTGGCT  >  NC_002947/1937056‑1937348
                                                                                                                                                 |                                                                                                                                                   
gCAGGGCGAAGATTTCCGGGTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATcc                                                                                                                                                  >  5:141028/1‑149 (MQ=255)
 cAGGGCGAAGATTTCCGGGTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCg                                                                                                                                                 <  1:207506/149‑1 (MQ=255)
            tttCCGGGTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCa                                                                                                                                         >  4:29498/1‑146 (MQ=255)
               ccGGGTCCTCGGTGCTGGCGTCGGCCACGTTCAGGTAGTTGACCGGCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTGCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGaa                                                                                                                                    <  8:252659/148‑1 (MQ=255)
                 gggTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGcc                                                                                                                                 >  1:217183/1‑149 (MQ=255)
                  ggTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGccc                                                                                                                                <  2:237126/149‑1 (MQ=255)
                     gATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAgg                                                                                                                             <  7:132450/149‑1 (MQ=255)
                      aTCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAgg                                                                                                                             <  4:70866/148‑1 (MQ=255)
                      aTCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAgg                                                                                                                             <  7:9174/148‑1 (MQ=255)
                          gTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTg                                                                                                                        >  8:311443/1‑149 (MQ=255)
                            gCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGc                                                                                                                      >  5:140613/1‑149 (MQ=255)
                            gCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGACTAGCACCGGCAGCATCAGGTATGGCCCATCCGGCACGCCATAGAAGCACAGGGTCTGGc                                                                                                                      >  1:389259/1‑149 (MQ=255)
                             cTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCGTCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           >  1:66491/1‑143 (MQ=255)
                             cTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCGTCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           <  2:226717/143‑1 (MQ=255)
                             cTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCGTCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           <  2:66491/143‑1 (MQ=255)
                             cTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCGTCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           >  1:226717/1‑143 (MQ=255)
                             cTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGc                                                                                                                      >  7:236354/1‑148 (MQ=255)
                                gCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCaaa                                                                                                                  <  7:22194/149‑1 (MQ=255)
                                gCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCaaa                                                                                                                  <  8:234402/149‑1 (MQ=255)
                                gCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCAAGGGTCTGGCCa                                                                                                                    >  8:8336/1‑147 (MQ=255)
                                 cTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAg                                                                                                                 >  5:375450/1‑149 (MQ=255)
                                       ccACGTGCAGGTAGTTGACCGACTGCTCTACCGCATAGTCCACCACCATCCCGGTTGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCg                                                                                                           <  7:259503/149‑1 (MQ=255)
                                         aCGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           <  2:8733/131‑1 (MQ=255)
                                         aCGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGt                                                                                                                           >  1:8733/1‑131 (MQ=255)
                                         aCGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGct                                                                                                         >  8:268354/1‑149 (MQ=255)
                                           gTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGctct                                                                                                       >  2:125667/1‑149 (MQ=255)
                                                          cGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCa                                                                                        >  2:14306/1‑149 (MQ=255)
                                                          cGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCa                                                                                        >  6:8092/1‑149 (MQ=255)
                                                            ccTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATg                                                                                      >  5:66677/1‑149 (MQ=255)
                                                             cTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGc                                                                                     <  6:375450/149‑1 (MQ=255)
                                                                 tctcCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGt                                                                                 >  6:228632/1‑149 (MQ=255)
                                                                     ccGCATAGTCCACCACCAGCCCGGTGGTATCACGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGcc                                                                             >  1:237859/1‑149 (MQ=255)
                                                                         atatTCCACCTCCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGt                                                                         <  2:92533/149‑1 (MQ=255)
                                                                         aTAGTCCACCACCAGCCCGGGGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGt                                                                         <  3:18020/149‑1 (MQ=255)
                                                                            gTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTccc                                                                      <  7:311443/149‑1 (MQ=255)
                                                                               caccaccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCcaca                                                                   <  3:156009/149‑1 (MQ=255)
                                                                                accaccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAg                                                                  >  5:242550/1‑149 (MQ=255)
                                                                                 ccaccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGc                                                                 <  5:228632/149‑1 (MQ=255)
                                                                                 ccaccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGc                                                                 <  6:140613/149‑1 (MQ=255)
                                                                                  caccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGcc                                                                <  6:141028/149‑1 (MQ=255)
                                                                                    ccaGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGccca                                                              <  8:78847/149‑1 (MQ=255)
                                                                                       gCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGcccaccc                                                           <  2:217183/149‑1 (MQ=255)
                                                                                            gtggtATCGCGCAGGTTCGATTGGCCTATCACCGGCATCATCATGTACGTCCCATCCGGCAAGCCTTAGAAGCCCAGGGTCTGGACAAAGTCCTCTCTCTGGCTCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCAACCACGcc                                                      <  8:120212/149‑1 (MQ=255)
                                                                                                      gcAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTTTGGCCCATCCGGGACGCCATAGTATCCGAGGGTCTGGGCAAAGTCCTCGCTGTGGCGCGGCAGGCCGATGCTGGTCGCCGGGTCCCAGAGGCGACCCACGCCGATGATAgtg                                            <  2:389259/149‑1 (MQ=255)
                                                                                                       cAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTTGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCgtgt                                           <  7:268354/149‑1 (MQ=255)
                                                                                                         ggTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGtt                                          >  7:359559/1‑148 (MQ=255)
                                                                                                          gTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTgg                                                                                  <  5:82240/107‑1 (MQ=255)
                                                                                                          gTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTgg                                                                                  >  6:82240/1‑107 (MQ=255)
                                                                                                             cGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAAc                                      <  4:86705/148‑1 (MQ=255)
                                                                                                                  ggCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGt                                >  1:132416/1‑149 (MQ=255)
                                                                                                                       aGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTcgcgc                           >  1:23633/1‑149 (MQ=255)
                                                                                                                          aCCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAg                                                                  >  4:355376/1‑107 (MQ=255)
                                                                                                                          aCCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAg                                                                  <  3:355376/107‑1 (MQ=255)
                                                                                                                              gcagcaTCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGAtt                    >  4:213587/1‑149 (MQ=255)
                                                                                                                                  caTCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTg                >  4:190227/1‑149 (MQ=255)
                                                                                                                                   aTCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGc               <  5:8092/149‑1 (MQ=255)
                                                                                                                                    tCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGcc              >  7:359456/1‑149 (MQ=255)
                                                                                                                                            ggCCCATCCGGCACGCCATAGAAGCCCAGGTTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGCCGAGCGCtt      <  1:14306/149‑1 (MQ=255)
                                                                                                                                              cccATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGCCGAGCGCTTgg    >  3:37855/1‑149 (MQ=255)
                                                                                                                                                cATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGCCGAGCGCTTGGCt  >  7:278427/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCAGGGCGAAGATTTCCGGGTGATCGGTGCTGGCTTCGGCCACGTTCAGGTAGTTGACCGCCTGCTCTCCCGCATAGTCCACCACCAGCCCGGTGGTATCGCGCAGGTTCGATGGGCCTAGCACCGGCAGCATCAGGTACGGCCCATCCGGCACGCCATAGAAGCCCAGGGTCTGGCCAAAGTCCTCGCTCTGGCGCGGCAGGCCCATGCTGGTCGCCGGGTCCCACAGCCCACCCACGCCGATGATCGTGTTGAACATCAGTCGCGCGGTGATTTCTGCCGAGCGCTTGGCT  >  NC_002947/1937056‑1937348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑