Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,938,844 T→C 9.0% E71G (GAG→GGG)  PP_1738 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,938,8440TC 9.0% 218.4 / 9.5 78E71G (GAG→GGG) PP_1738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (39/32);  new base C (3/4);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

TTTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATC  >  NC_002947/1938697‑1938986
                                                                                                                                                   |                                                                                                                                              
tttttCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTc                                                                                                                                               >  7:365017/1‑149 (MQ=255)
    tCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCgg                                                                                                                                            <  5:90421/148‑1 (MQ=255)
       ggTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                         >  5:321022/1‑148 (MQ=255)
           ttCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGt                                                                                                                                    >  6:95786/1‑149 (MQ=255)
             cATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGtt                                                                                                                                   >  6:169364/1‑148 (MQ=255)
              aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGtt                                                                                                                                   >  3:25269/1‑147 (MQ=255)
              aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCa                                                                                                                                 <  1:313704/149‑1 (MQ=255)
              aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCa                                                                                                                                 <  3:174264/149‑1 (MQ=255)
              aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATAAGCGGCGCTTCCTGATCCTCGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCATGAAGTTGTAGGGCAACGAGAACTCGCGATACGGGCGCAAGTACa                                                                                                                                 >  6:279855/1‑149 (MQ=255)
              aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGAGGATGAAGAGCAGCGGCGCTTCGTGATCGTGGCGGGCCAGGCGGTACTTGAGCTGCTTCACTGGCCAGAAGTGGTCGGGCGACGTGGGGTCGGGCTCGGGgcggcgggg                                                                                                                                   >  6:202405/1‑141 (MQ=255)
                 gATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATTATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAgg                                                                                                                               <  7:364503/148‑1 (MQ=255)
                   tgctgctgcTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTCacgttg                                                                                                                            <  6:174005/149‑6 (MQ=255)
                   tgctgctgcTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCGTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCGTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTg                                                                                                                            <  8:155912/149‑1 (MQ=255)
                    gctgctgctGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTcc                                                                                                                                              >  6:51913/1‑130 (MQ=255)
                    gctgctgctGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTcc                                                                                                                                              <  5:51913/130‑1 (MQ=255)
                    gctgctgctGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTg                                                                                                                            >  6:253257/1‑148 (MQ=255)
                    gctgctgctGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTg                                                                                                                            >  8:364749/1‑148 (MQ=255)
                             gTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                  >  5:64101/1‑149 (MQ=255)
                              tAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGAc                                                                                                                   <  1:370884/147‑1 (MQ=255)
                              tAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                  <  6:184728/148‑1 (MQ=255)
                               catcatcgcCGGCACCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTACACTGCCCAGAAGTTTTCGGGCAACGTGAACTCGCGCTCCCGGCGCAAGATCAGGCTGTAGTCGGACt                                                                                                                  >  4:270200/7‑147 (MQ=255)
                                gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                               <  5:326995/149‑1 (MQ=255)
                                gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                               <  5:317977/149‑1 (MQ=255)
                                          gTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtc                                                                                                     >  5:15207/1‑149 (MQ=255)
                                              cGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtcgtcg                                                                                                 <  5:95786/149‑1 (MQ=255)
                                                 cGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTg                                                                                              <  7:364749/149‑1 (MQ=255)
                                                      attaaGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCa                                                                                          <  5:305927/145‑1 (MQ=255)
                                                            aTCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAgg                                                                                   <  8:365017/149‑1 (MQ=255)
                                                              cAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAggg                                                                                  <  3:34443/148‑1 (MQ=255)
                                                               aGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGgc                                                                                 <  6:378142/148‑1 (MQ=255)
                                                                 cggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAATTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCt                                                                              >  8:175252/1‑149 (MQ=255)
                                                                        tCGTGATCCTGGCGTGCCAGGCGGTACTTGAGCTTCTTCTCTTCGCAGAAGTTGTCGGTCAACTTGATCTCGCGCTCTGTTGGCAAGTTCAGGCTGTAGTGTTTCTGAGTGTTTTCATCGTCGGTGGGCTGCTCAGGGGGCtttttcg                                                                        <  8:8051/148‑6 (MQ=255)
                                                                          gTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCTGGCGCTAGTTCAGGCTGTAGTCGGACTGAGTCATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggc                                                                      <  5:279855/148‑1 (MQ=255)
                                                                          gTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcgtcg                                                                     >  5:16420/1‑149 (MQ=255)
                                                                          gTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggcg                                                                     <  6:64101/149‑1 (MQ=255)
                                                                          gTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggcg                                                                     >  1:230412/1‑149 (MQ=255)
                                                                           tGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggcgg                                                                    >  2:92687/1‑149 (MQ=255)
                                                                            gATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCggcggc                                                                   >  6:164914/1‑149 (MQ=255)
                                                                              tCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCgt                                                                 >  6:304016/1‑149 (MQ=255)
                                                                                cTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCgtgg                                                               <  2:230412/149‑1 (MQ=255)
                                                                                cTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCgtg                                                                <  5:169364/148‑1 (MQ=255)
                                                                                 tGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGggtggt                                                              >  8:67494/1‑149 (MQ=255)
                                                                                  ggCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggc                                                                      <  1:60068/140‑1 (MQ=255)
                                                                                  ggCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggc                                                                      >  2:60068/1‑140 (MQ=255)
                                                                                           ggCGGTACTTGAGCTTCTTCACTGCCCAGAAGTGGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAtggtg                                                    <  5:304016/149‑1 (MQ=255)
                                                                                                     gAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACg                                          >  7:364623/1‑149 (MQ=255)
                                                                                                     gAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACg                                          >  6:292246/1‑149 (MQ=255)
                                                                                                            ttcACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTgg                                    >  2:238831/1‑148 (MQ=255)
                                                                                                             tcACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGt                                   >  7:216696/1‑148 (MQ=255)
                                                                                                              cACTGCCCAGAAGTTGTCGGGCAACGTGAACTCCCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGt                                   <  4:25269/147‑1 (MQ=255)
                                                                                                               aCTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGt                                   >  1:45993/1‑146 (MQ=255)
                                                                                                                cTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTaa                                 >  4:376498/1‑147 (MQ=255)
                                                                                                                cTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAaga                               <  6:15207/149‑1 (MQ=255)
                                                                                                                cTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAaga                               <  8:216696/149‑1 (MQ=255)
                                                                                                                 tGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGaa                                            >  6:335222/1‑135 (MQ=255)
                                                                                                                 tGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGaa                                            <  5:335222/135‑1 (MQ=255)
                                                                                                                    ccAGAAGTTGTCGGGCAACGTGAACTAGCGCTCCGGGCGCAAGTTCAGGCTCTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGCCGCTTGTCCGGCGGCGTTGATGAGATGGAGTCCTCGAACGGATTGCTAAGAggt                            >  6:271324/1‑147 (MQ=255)
                                                                                                                      aGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTTGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAg                         <  3:270200/149‑1 (MQ=255)
                                                                                                                      aGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAg                         >  7:46875/1‑149 (MQ=255)
                                                                                                                           ttGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAACGTCGCTGGGTAGCTCAGGTCGCTTTTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCAt                     <  5:271324/148‑1 (MQ=255)
                                                                                                                              tCGGGCAACGTGAATTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCt                 <  7:175252/149‑1 (MQ=255)
                                                                                                                              tCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGCTACCCATAGCt                 >  7:187053/1‑149 (MQ=255)
                                                                                                                                gggCCACGTGATCTAGCGCTCCGGGCGCAAGTTCAGGCTGTAGGCGGACTGAGTGTTTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg               <  8:187053/149‑1 (MQ=255)
                                                                                                                                 ggCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg               >  8:238409/1‑148 (MQ=255)
                                                                                                                                  gCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg               >  3:154261/1‑147 (MQ=255)
                                                                                                                                   cAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAg            >  5:381906/1‑149 (MQ=255)
                                                                                                                                   cAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAg            >  5:387072/1‑149 (MQ=255)
                                                                                                                                    aaCGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGc           <  5:292246/149‑1 (MQ=255)
                                                                                                                                      cctGAACTTGCGCCCGGAGCGCGAGTTCACGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggcg                                                                     >  3:238923/3‑89 (MQ=25)
                                                                                                                                      cctGAACTTGCGCCCGGAGCGCGAGTTCACGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggcg                                                                     <  4:238923/87‑1 (MQ=25)
                                                                                                                                       gTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTc        >  4:43714/1‑149 (MQ=255)
                                                                                                                                       gTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTc        >  2:270179/1‑149 (MQ=255)
                                                                                                                                        tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGACAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTg                                     <  7:5429/119‑1 (MQ=255)
                                                                                                                                        tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGACAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTg                                     >  8:5429/1‑119 (MQ=255)
                                                                                                                                         gAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGt                                       >  3:178728/1‑116 (MQ=255)
                                                                                                                                         gAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGt                                       <  4:178728/116‑1 (MQ=255)
                                                                                                                                            cTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGCCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGatct   >  7:161803/1‑147 (MQ=255)
                                                                                                                                             tCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATc  >  5:351976/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TTTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATC  >  NC_002947/1938697‑1938986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑