Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,975,985 A→G 7.3% Y306C (TAT→TGT) ‡ pheA → bifunctional chorismate mutase/prephenate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,975,9850AG 7.3% 194.8 / 4.3 68Y306C (TAT→TGT) ‡pheAbifunctional chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (36/27);  new base G (3/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTG  >  NC_002947/1975842‑1976128
                                                                                                                                               |                                                                                                                                               
gaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAg                                                                                                                                             >  6:313608/1‑148 (MQ=255)
   cGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACg                                                                                                                                         >  1:384430/1‑149 (MQ=255)
   cGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACg                                                                                                                                         >  2:260864/1‑149 (MQ=255)
       gcccGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCac                                                                                                                                     <  2:384430/147‑2 (MQ=255)
        aCCGCCCGGAAAACTCCACGCGCTTCCTGATGATGGGTAACCTTGAAGTACCGCCGTCCGGCGACGACAAGACCTCGATCATCGTTTCCATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCacc                                                                                                                                    <  2:355241/149‑3 (MQ=255)
            cccGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGAcc                                                                                                                                >  6:304924/1‑149 (MQ=255)
             ccGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCt                                                                                                                               <  6:209229/149‑1 (MQ=255)
                       ccACGCTCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc                                                                                                                     <  5:192692/149‑1 (MQ=255)
                        cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc                                                                                                                     >  7:165042/1‑148 (MQ=255)
                        cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAAGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCAt                                                                                                                      <  6:247789/147‑1 (MQ=255)
                         aCGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTa                                                                                                                                                 <  2:32151/119‑1 (MQ=255)
                         aCGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTa                                                                                                                                                 >  1:32151/1‑119 (MQ=255)
                             gcTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTTCATGAGTTGCTGGTGACGTTCTATCAGAACGGCATCGAAATTACCCGCATCTAGAcc                                                                                                               >  5:156309/1‑149 (MQ=255)
                               ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccg                                                                                                             >  8:249245/1‑149 (MQ=255)
                               ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAccc                                                                                                              >  7:332395/1‑148 (MQ=255)
                                 ccTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAccc                                                                                                              <  8:332395/146‑1 (MQ=255)
                                      tgatCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTcg                                                                                                      >  7:214873/1‑149 (MQ=255)
                                        atCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTcgcg                                                                                                    >  8:162575/1‑149 (MQ=255)
                                              aaCCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCgg                                                                                              >  2:20851/1‑149 (MQ=255)
                                               aCCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             <  3:177550/149‑1 (MQ=255)
                                                ccAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  5:250456/1‑148 (MQ=255)
                                                ccAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGACAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  1:383456/1‑148 (MQ=255)
                                                 cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  1:342821/1‑147 (MQ=255)
                                                 cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  6:50083/1‑147 (MQ=255)
                                                 cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  1:164808/1‑147 (MQ=255)
                                                 cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  3:54674/1‑147 (MQ=255)
                                                 cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGc                                                                                             >  8:111861/1‑147 (MQ=255)
                                                   ggAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGt                                                                                         >  7:340282/1‑149 (MQ=255)
                                                       ttACCTCCGACCGGCTACGAGAAGACCTCGATCTTGTTTTCGATGAGCAACAAGCCAGGTGCGTTGCATTAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTTACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGAc                                                                                     <  6:156309/148‑1 (MQ=255)
                                                           cgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTGCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc                                                                                 <  5:313608/149‑1 (MQ=255)
                                                            gccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACg                                                                                >  1:117618/1‑149 (MQ=255)
                                                             ccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgt                                                                               >  1:33088/1‑149 (MQ=255)
                                                              cgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgtg                                                                              <  8:165042/149‑1 (MQ=255)
                                                               gACCGGCGACGACAAGACCCCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgtgt                                                                             >  4:124824/1‑149 (MQ=255)
                                                                 ccGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttc                                                                           <  7:115408/149‑1 (MQ=255)
                                                                    gCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCCGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttcttc                                                                        <  8:340282/149‑1 (MQ=255)
                                                                      gacgacAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCAt                                                                      <  3:249127/149‑1 (MQ=255)
                                                                      gacgacAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCAt                                                                      <  6:250456/149‑1 (MQ=255)
                                                                       acgacAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATc                                                                     <  7:249245/149‑1 (MQ=255)
                                                                             aGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtc                                                               <  1:260864/149‑1 (MQ=255)
                                                                                 cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTCCGTGTTCTTCATCGACTtcgtcg                                                           >  6:93133/1‑149 (MQ=255)
                                                                                 cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc                                                                                 <  4:45493/127‑1 (MQ=255)
                                                                                 cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTAc                                                                                 >  3:45493/1‑127 (MQ=255)
                                                                                 cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTtcgtcg                                                           <  5:304924/149‑1 (MQ=255)
                                                                                     atcatcGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGcca                                                       >  5:233169/1‑149 (MQ=255)
                                                                                           gTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcg                                                 <  7:111861/149‑1 (MQ=255)
                                                                                            tttCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcgc                                                >  7:174928/1‑149 (MQ=255)
                                                                                                    aGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc                                         <  2:164808/148‑1 (MQ=255)
                                                                                                    aGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc                                         <  1:20851/148‑1 (MQ=255)
                                                                                                    aGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCt                                        <  2:33088/149‑1 (MQ=255)
                                                                                                     gCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCt                                        >  3:1523/1‑148 (MQ=255)
                                                                                                       aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACATGACCCGCATCGAGACCCGCCCGTCGCGCAGAGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGTCCACCAACGCGACCCGCTGa                                      >  3:178706/1‑148 (MQ=255)
                                                                                                            gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAgg                                <  2:71774/149‑1 (MQ=255)
                                                                                                               aGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAgg                                >  8:345144/1‑146 (MQ=255)
                                                                                                               aGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAgg                                <  7:345144/146‑1 (MQ=255)
                                                                                                               aGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCgg                             >  8:106552/1‑149 (MQ=255)
                                                                                                                     gTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACGCGCCCGTCGCGCAGCGGCAAGTGGCCCTACGGGTTCGTCATCGACTTCGGCGGCCACCCCCGCGCCCCGCGGATCAAGGCGGTGCTCg                       >  4:74729/1‑149 (MQ=255)
                                                                                                                      ttGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGa                      <  8:174928/149‑1 (MQ=255)
                                                                                                                        gCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg                     <  8:214873/148‑1 (MQ=255)
                                                                                                                         cATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg                     >  2:259023/1‑147 (MQ=255)
                                                                                                                         cATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg                     >  1:62054/1‑147 (MQ=255)
                                                                                                                              gTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGAt                >  7:145216/1‑147 (MQ=255)
                                                                                                                              gTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCa              <  1:259023/149‑1 (MQ=255)
                                                                                                                               ttGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACGTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAg             >  1:123218/1‑149 (MQ=255)
                                                                                                                                  tcgatGCCGTTCTGATAGAACGGCACCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGc            >  4:266813/5‑147 (MQ=255)
                                                                                                                                  cTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCa          <  8:119877/149‑1 (MQ=255)
                                                                                                                                   tGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCag         >  1:350611/1‑149 (MQ=255)
                                                                                                                                       gCCGTTCTGATAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCaggagg     >  7:147805/1‑149 (MQ=255)
                                                                                                                                         cGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCt   <  2:117618/149‑1 (MQ=255)
                                                                                                                                          gTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtg  >  5:107701/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTG  >  NC_002947/1975842‑1976128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑