Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,053,642	G→A	10.7%	intergenic (‑42/+24)	PP_1828 ← / ← PP_1829	hypothetical protein/alpha/beta family hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,053,642	0	G	A	10.7%	177.7 / 8.6	66	intergenic (‑42/+24)	PP_1828/PP_1829	hypothetical protein/alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base A (3/4);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCGCAGC  >  NC_002947/2053495‑2053789                                                                                                                                                    |                                                                                                                                                     ccAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATgg                                                                                                                                                     >  1:302406/1‑149 (MQ=255)    ggCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCt                                                                                                                                                   >  8:101456/1‑148 (MQ=255)     gCGTAGCAGCTGGAAATGTTCGGCGGTCTGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCCATGCCTGCCGATACAGCAGGCATAGCTga                                                                                                                                                 <  2:256098/149‑1 (MQ=38)      cGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTgag                                                                                                                                                <  2:31783/149‑1 (MQ=255)       gTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTgaga                                                                                                                                               >  5:12149/1‑149 (MQ=255)            agcTGTAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGa                                                                                                                                          <  5:301/149‑1 (MQ=255)                       ttgggcggTCCGCGCGGCCCCGGGCAGGGCGGGGGTTTCCTGGGGTGCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGAc                                                                                                                              <  8:76447/146‑1 (MQ=255)                       ttCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGAc                                                                                                                              >  6:17345/1‑149 (MQ=255)                       ttCGGCGGTCAGCCCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGAc                                                                                                                              >  7:355770/1‑149 (MQ=255)                          ggcggGCCGCGCGGCCCCGGGCTGGGCGGGGGTTTCCTGGGGTTCTGCGGCCGGCGCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTa                                                                                                                           <  3:220385/149‑1 (MQ=255)                          ggcggGCAGCGCGGCCCAGGGCTGGGGGGGGGTTTCGTGGGGTTCTTCGGCCGGCGCGACGGCGGTGGGGGTGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTa                                                                                                                           <  3:215392/149‑1 (MQ=255)                                cAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCg                                                                                                                      >  7:311374/1‑148 (MQ=255)                                 aGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGaa                                                                                                                    <  2:340447/149‑1 (MQ=255)                                  gCTCGGCCCCGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGcc                                                                                                                       >  5:200797/1‑145 (MQ=255)                                  gCTCGGCCCCGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGcc                                                                                                                       <  6:200797/145‑1 (MQ=255)                                   cTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAgg                                                                                                                  >  2:383641/1‑149 (MQ=255)                                    tCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAgg                                                                                                                  >  5:103186/1‑148 (MQ=255)                                    tCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTAGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAgg                                                                                                                  >  5:386225/1‑148 (MQ=255)                                             ggcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTg                                                                                                         >  8:102708/1‑148 (MQ=255)                                              gcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGa                                                                                                       <  7:101456/149‑1 (MQ=255)                                              gcagggcGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGa                                                                                                       <  6:103186/149‑1 (MQ=255)                                               ctggggggggGTTTCCTGGGGTTCTTCGGCCGGCTCGAGGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGAt                                                                                                      <  2:289023/147‑1 (MQ=255)                                                   gggggggTTTCCTGGTGTTCTTCGGCCGGATCTACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCATCTATGCATTCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAAc                                                                                                  <  2:132751/147‑1 (MQ=255)                                                    cGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACg                                                                                                 >  5:144230/1‑149 (MQ=255)                                                     gggggTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGt                                                                                                >  3:200866/1‑149 (MQ=255)                                                     gggggTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGt                                                                                                <  6:12149/149‑1 (MQ=255)                                                        ggTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTcac                                                                                             <  5:17345/149‑1 (MQ=255)                                                              cTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcg                                                                                       >  7:171558/1‑149 (MQ=255)                                                                 gggTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATtcgctc                                                                                    >  8:200991/1‑149 (MQ=255)                                                                    ttcttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTcac                                                                                             <  2:256937/137‑1 (MQ=255)                                                                    ttcttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTcac                                                                                             >  1:256937/1‑137 (MQ=255)                                                                      cgacggccggcGCGACGGCGGGGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCTCCCa                                                                               <  2:302406/146‑1 (MQ=255)                                                                       ttcGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAg                                                                              >  6:226415/1‑149 (MQ=255)                                                                            ccggcTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCAc                                                                         <  8:86537/149‑1 (MQ=255)                                                                                      ggcggTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCgg                                                               <  4:165645/149‑1 (MQ=255)                                                                                                    tggtCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGa                                                 <  6:144230/149‑1 (MQ=255)                                                                                                        catcaGGGGTTGTCCTCAGATATTCCTGCTGTATCTGCAGGCATTGCTGAGACCTGAGA‑CTTTTTGACTTAGGCCGAAGTCAAATGCTGGATTAACGTCACAATTCGCTCGCCCTGCTCACGCGCCAGGGGAAGCTGGGGGTTGAGGt                                              <  6:27342/148‑1 (MQ=255)                                                                                                         atcaGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAg                                            >  8:104394/1‑149 (MQ=255)                                                                                                         atcaGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAg                                            <  8:171558/149‑1 (MQ=255)                                                                                                             gggCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGt                                        >  1:81289/1‑149 (MQ=255)                                                                                                              ggCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGCGCTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGt                                        >  1:296663/1‑149 (MQ=255)                                                                                                                     ccTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgc                                >  6:297355/1‑149 (MQ=255)                                                                                                                      cTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgct                               >  7:109488/1‑149 (MQ=255)                                                                                                                      cTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgc                                <  8:311374/148‑1 (MQ=255)                                                                                                                       tCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCGGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCCGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGctgctg                              >  1:297640/1‑149 (MQ=255)                                                                                                                       tCAGCCATGCCTGCCGATACAGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGctgctg                              >  7:45366/1‑149 (MQ=255)                                                                                                                        cAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTcgc                                     >  7:119595/1‑141 (MQ=38)                                                                                                                        cAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTcgc                                     <  8:119595/141‑1 (MQ=38)                                                                                                                        cAGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAACGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTgg                             >  8:274832/1‑149 (MQ=38)                                                                                                                         aGCCATGCCTGCCGATACAGCAGGCATAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGc                            >  7:252730/1‑149 (MQ=255)                                                                                                                             ctGCCTGCTGTATCGCCAGGCATGGCTGAGACCTGAGC‑CGGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCAt                        <  8:347027/148‑1 (MQ=255)                                                                                                                             aTGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGTACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               <  1:259304/126‑1 (MQ=255)                                                                                                                             aTGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAgg                                               >  2:259304/1‑126 (MQ=255)                                                                                                                                ccTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGcc                     >  6:22518/1‑149 (MQ=255)                                                                                                                                 ctgctgTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAACTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGccc                    >  1:312948/1‑149 (MQ=255)                                                                                                                                      gTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTggg               >  6:213579/1‑149 (MQ=255)                                                                                                                                       tagcGGCAGGCATGTCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAACTGCTGGAGGCACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGGTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTg                 <  2:297640/143‑1 (MQ=255)                                                                                                                                       tATCGGGAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATCAACGTCACAATTCGCTCCCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgg                <  6:386225/147‑1 (MQ=255)                                                                                                                                       tATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgggg              >  7:32675/1‑149 (MQ=255)                                                                                                                                       tATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgggg              <  2:296663/149‑1 (MQ=255)                                                                                                                                       tATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgg                <  7:200991/147‑1 (MQ=255)                                                                                                                                       tATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTgg                <  7:102708/147‑1 (MQ=255)                                                                                                                                         tCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgc                                <  1:61369/129‑1 (MQ=255)                                                                                                                                         tCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCgctgc                                >  2:61369/1‑129 (MQ=255)                                                                                                                                          cagcaggcaTAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCt            <  8:252730/146‑1 (MQ=255)                                                                                                                                          cagcaggcaTAGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCACCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCt            <  6:257526/146‑1 (MQ=255)                                                                                                                                           ggcaggcaTGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGa          <  8:355770/149‑1 (MQ=255)                                                                                                                                                    ggCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCgcagc  <  8:45366/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCAGGCGTAGCAGCTGGAAATGTTCGGCGGTCAGCTCGGCCCAGGGCAGGGCGGGGGTTTCCTGGGGTTCTTCGGCCGGCTCGACGGCGGTGGGGGAGGTGGTCATCAGGGCTTGTCCTCAGCTATGCCTGCTGTATCGGCAGGCATGGCTGAGACCTGAGC‑CTGTTTGACTTACGCCGAAGGCAAATGCTGGATGAACGTCACAATTCGCTCGCCCAGCTCACGCGCCAGCGGCAGCTCGGGGTTGAGGTAGCTGTCGCGCTGCTGGCTCATGCCCTTGGGGCTGATGCGCAGC  >  NC_002947/2053495‑2053789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑