| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,075,430 | G→T | 9.7% | intergenic (‑52/‑55) | ygaY ← / → PP_5479 | transporter YgaY/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,075,430 | 0 | G | T | 9.7% | 104.8 / 2.1 | 39 | intergenic (‑52/‑55) | ygaY/PP_5479 | transporter YgaY/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (18/16); new base T (2/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTGGTTGGGCGTAATAGTTGCTGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCATGTGGTGCAGT > NC_002947/2075286‑2075572 | cagTGGTTGGGCGTAATAGTTGCTGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCATGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCAt < 8:354190/149‑1 (MQ=255) gTTGGGCGAAATAGGTGCTGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCATGATCAAGCCGCGGCTGACAGGGGTGGTCTGCATGCAATGCTCGCGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGacac < 7:357261/149‑1 (MQ=255) tGGGCGTAATAGTTGCTGGCCACGGTCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCATGCCTGacact > 7:219076/1‑147 (MQ=255) ggCGTAATAGTTGCGGTCCTCGGCCAGGCCTGTGGCGGTGGCCATCAGCAGTATCCAGGCGCGGCTGACAGTGCGGGCGTGCATGCAATTGTCGTGGGGGCTGTGTTGTGTTGTGTTGATTATCAGTATGTGTCATGCATGACTCCAAt < 2:286146/149‑1 (MQ=255) ggCGGACTAGTTGCTGCCCACGGTCAGGCCTGTGCCGGTGGCCATCAGCAGGCTCAAGGCGCGGCTGAGAGTGGGCGGGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCATGCCTGacacttac < 8:219076/149‑5 (MQ=255) cTGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCCAGGCGCGGCTGCGAGTGGGGGTGTGCATGCAATTCTCTTGGTCGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑a < 3:220027/148‑1 (MQ=255) tGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGc > 7:315506/1‑149 (MQ=255) gCCACGGCCAGGCCTGTGGCGGTGGCCTTCAGCAGGATCATGGCGCGGCTGAGAGGCGGGGAGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGc < 3:236066/147‑1 (MQ=255) cACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGAATGACACCAATGTATAGTTTTC‑AGACTAt > 7:264065/1‑149 (MQ=255) aGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCTATGTATAGTTTTC‑AGCCTATTCATCtt < 6:128263/149‑1 (MQ=255) gCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTg > 7:123852/1‑148 (MQ=255) tCAGCAGGATCAAGGCGCGGCTGAGAGTCGGCGTGTCCATGCAATTCTCGTGGCCGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATg < 1:127399/149‑1 (MQ=255) ggCTGAGACTCGGGGAGGTCATGCAACTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGaa < 2:228256/149‑1 (MQ=255) atgcaATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCa < 8:170956/57‑1 (MQ=255) atgcaATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCa > 7:170956/1‑57 (MQ=255) tgcaATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGa > 8:318624/1‑148 (MQ=255) gcaATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGaca > 7:196402/1‑149 (MQ=255) ttCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGACCTTGCCGTGGCCGACACCgg > 1:318312/1‑149 (MQ=255) tcGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGc > 2:79774/1‑72 (MQ=255) tcGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGATAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAg > 5:199458/1‑149 (MQ=255) gTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAg < 2:359288/147‑1 (MQ=255) ggtggCAGTGGTGTTTAGTGATGATTATCAGTATGTTTCAGGCATGACACAAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCagatagat < 4:311657/90‑1 (MQ=255) gggggCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTCTAGCATATTCATCTTGAAAGAAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGttt > 1:116199/1‑149 (MQ=255) gggggCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCagatagat > 3:311657/1‑93 (MQ=255) ggCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTAcc > 5:288547/1‑149 (MQ=255) tgtgtAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGTCCGACACCGGCAGTTTTACCCGCgcggc < 8:123852/149‑1 (MQ=255) gtgtAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCgcggcg > 8:324922/1‑149 (MQ=255) gtggAGTTATGATTATCAGTCAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCgcggc < 8:264065/148‑1 (MQ=255) tgtAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTAATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCgcggcg > 5:235999/1‑148 (MQ=255) tAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATTAAc < 1:10532/86‑1 (MQ=255) tAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTAC‑AGCCTATTCATCTTGAAAGCAGATACATCGATGAAc > 2:10532/1‑86 (MQ=255) tgatgaTTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGcc < 5:85771/116‑1 (MQ=255) tgatgaTTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGcc > 6:85771/1‑116 (MQ=255) atTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGcc < 8:315506/148‑1 (MQ=255) ttATAAGTAAGTGACACGCATGACACCAATGTATAGTTTTC‑AGCCTATTCAACTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCa < 8:326872/148‑1 (MQ=255) tCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCAtgtg > 5:195921/1‑149 (MQ=255) aGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCATgtggt < 6:161208/149‑1 (MQ=255) ttggtgtCATGCCTGACACCAAAGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCACTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCATgtggtg > 8:256564/4‑148 (MQ=255) gtgtCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCATGTGGTGCAGt > 1:355571/1‑149 (MQ=255) | CAGTGGTTGGGCGTAATAGTTGCTGGCCACGGCCAGGCCTGTGGCGGTGGCCATCAGCAGGATCAAGGCGCGGCTGAGAGTGGGGGTGTGCATGCAATTCTCGTGGGGGCAGTGGTGTGTAGTGATGATTATCAGTAAGTGTCAGGCATGACACCAATGTATAGTTTTC‑AGCCTATTCATCTTGAAAGCAGATAGATCGATGAACCTCAAGCAGCTCGAATATGCCCTTGCCGTGGCCGACACCGGCAGTTTTACCCGCGCGGCGGAGCGCTGCCATGTGGTGCAGT > NC_002947/2075286‑2075572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |