Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,084,112 T→A 10.4% intergenic (+16/+13) PP_1862 → / ← PP_1863 membrane protein/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,084,1120TA10.4% 100.0 / 2.4 38intergenic (+16/+13)PP_1862/PP_1863membrane protein/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (18/16);  new base A (2/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATG  >  NC_002947/2083969‑2084251
                                                                                                                                               |                                                                                                                                           
cGGTGGGTGGCTTGCTCTGGATCAGCTGGGGTGTGAAGCTTGCCCATGGTTTGCCGGTGAAGGTATTGCGGGCTATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTctg                                                                                                                                        <  6:148182/149‑3 (MQ=255)
    gggtggCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGccc                                                                                                                                     >  7:72739/1‑148 (MQ=255)
     ggtggCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAAAATCGGcccc                                                                                                                                    <  2:306184/148‑1 (MQ=255)
      gtggCTTGCTGAGCATCAGCTGGGGTGTGAAGCTGGGCCATGCGTTGCAGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGTCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAg                                                                                                                                  <  7:45027/149‑1 (MQ=255)
             ggtCAGCATCAGCTGGTGTGTGAAGGTGGCCAATGCGTTGCCGGAGTAGGTATTGCGTGTGATGTTCTGTGTGTTCGTGGTGGTCTGTGCGGTTATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACt                                                                                                                            <  5:109095/146‑1 (MQ=255)
                       aGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTGCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGGCGATTTTCGGCGCCAGATCACTTGCAAGCCTc                                                                                                                  >  8:229731/1‑148 (MQ=255)
                        gCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCaa                                                                                                                <  5:118370/149‑1 (MQ=255)
                             ggTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATg                                                                                                           >  4:259340/1‑149 (MQ=255)
                              gtgtGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATg                                                                                                           >  8:33755/1‑148 (MQ=255)
                               tgtgAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTa                                                                                                         <  8:72739/149‑1 (MQ=255)
                                         gCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATg                                                                                               <  1:28452/149‑1 (MQ=255)
                                         gCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTATCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCAt                                                                                                >  7:50848/1‑148 (MQ=255)
                                                     ccGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGAt                                                                                    <  6:178976/148‑1 (MQ=255)
                                                            aGGTATAGCGGGCTATGGTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGATCTTTGAGCTTTTGGGCCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAgg                                                                            <  4:258932/149‑1 (MQ=255)
                                                             ggTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAGTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAggg                                                                           >  2:95718/1‑149 (MQ=255)
                                                              gTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAgggg                                                                          <  3:259340/149‑1 (MQ=255)
                                                              gTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCAAtggg                                                                          <  1:198763/149‑1 (MQ=255)
                                                                                 gtgtgtTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCACCAGATCACTTGAAACCCTCAACGATGTAGTCGGCAATGCAGTCGGTGATTGGCGATGGGGTCTGCGTACTGAGCAc                                                         >  3:229361/1‑147 (MQ=255)
                                                                                 gtgtcatCCTGGTGGTCTGTGCGGTCATGCTGCCGTTTGATCTTTGAGATTTTGGCGCCGATTTTCGGCCCCAGATCACTTGAAACACTCAACGATGTACTCGGCCATGAAGTCCGAGATTGGCGAGGGGGTCTGCGTACTGCGCaa                                                         >  8:65807/7‑146 (MQ=255)
                                                                                  tgtgtTCCTGGTTGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCa                                                       <  6:234949/148‑1 (MQ=255)
                                                                                  tgtgtTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAg                                                      >  7:100635/1‑149 (MQ=255)
                                                                                    tgtTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCa                                                    >  6:42875/1‑149 (MQ=255)
                                                                                      ttCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCAt                                                   >  8:78535/1‑148 (MQ=255)
                                                                                          tggtggTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGATACCCTCAACGATGTAGTCGGCCATGGAGTCGGTGATTGGCGAGGGGGTCTTCGTACTGCGCACCAGCTTTACAt                                              >  7:365392/1‑149 (MQ=255)
                                                                                            gtggtCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTg                                            >  6:133689/1‑149 (MQ=255)
                                                                                                        gTGATGCTGGCGTTTCCGCTTTGAGTTTTTGGGGGCGATTTTCGGGCCCAGATCTCTTGTAACCCTCAAGGTTGTAGTCGTCGATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGGATTACATTGGCCATGGGCAAc                                <  8:50848/149‑1 (MQ=255)
                                                                                                          gATGCTGGCGTTTGAGCGTTGAGATTTTGGGGCCGATTTTCGGCCCCCGATCACGTGAACCCCGCAACGAGGTAGGCGGCCGGGCAGGGGGGGGTTGGCGGGGGGGGCGGCGGACGGGGCCCCCGCAGGCCATTGGCCATGGGCAACTg                              >  2:152531/1‑149 (MQ=255)
                                                                                                               tGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCgg                           >  4:289924/1‑147 (MQ=255)
                                                                                                                        aGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCg                 <  8:100635/148‑1 (MQ=255)
                                                                                                                         gCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCAt                                                   >  2:7873/1‑113 (MQ=255)
                                                                                                                         gCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCAt                                                   <  1:7873/113‑1 (MQ=255)
                                                                                                                          cTTTGAGTTTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTGAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGCCCATGGGCAACTGCGGCAGCCCTTCGct               <  1:152531/148‑1 (MQ=255)
                                                                                                                              gagaTTTTGGGGCCGATATTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCa           <  7:263685/148‑1 (MQ=255)
                                                                                                                                 aTTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAcc         >  5:247101/1‑147 (MQ=255)
                                                                                                                                  ttttGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAcc         >  7:345951/1‑146 (MQ=255)
                                                                                                                                  atctGGGGCCGAAAATCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAcc         >  2:151501/4‑146 (MQ=255)
                                                                                                                                      ggggCCGAAAATCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATg  >  5:66593/1‑149 (MQ=255)
                                                                                                                                       gggCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATg  <  7:33755/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATG  >  NC_002947/2083969‑2084251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑