Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,084,299	C→G	5.9%	G226G (GGG→GGC)	PP_1863 ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,084,299	0	C	G	5.9%	215.0 / 3.6	68	G226G (GGG→GGC)	PP_1863	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base G (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

CATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATCGGCCGC  >  NC_002947/2084155‑2084443                                                                                                                                                  |                                                                                                                                                 cATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCcgg                                                                                                                                              >  6:334835/1‑149 (MQ=255) cATGCAGTCGGTGATTGGCGAGCGGGTCTGCGTACAGCGCACCAGCAATACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCcgg                                                                                                                                              <  8:365392/149‑1 (MQ=255)  aTGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCcggc                                                                                                                                             >  3:261586/1‑149 (MQ=255)   tGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCcggcg                                                                                                                                            <  7:313539/149‑1 (MQ=255)   tGCAGGCGGTGATTGGCGAGTGTGTCTGCGTACTGCGCACGAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAAGGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCcggcg                                                                                                                                            <  4:229361/149‑1 (MQ=255)       gTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGa                                                                                                                                        <  5:133689/149‑1 (MQ=255)       gTCGGTGATTGGCGAGGGGGGCGGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGGGCGGTCACCGCCGGGCTGGCAGTGa                                                                                                                                        <  4:204977/149‑1 (MQ=255)        tCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGAc                                                                                                                                       <  4:261586/149‑1 (MQ=255)          ggTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGa                                                                                                                                     <  7:229731/149‑1 (MQ=255)            tGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATg                                                                                                                                   <  3:289924/149‑1 (MQ=255)             gATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTTTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATgg                                                                                                                                  >  4:316241/1‑149 (MQ=255)              aTTGGCGTGGGGGTCTGCGTACTTCTCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAGCCAGAATGCGGATGTTGCGCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCGAGCCCGGCGGTGACGAttg                                                                                                                                  <  4:290696/148‑3 (MQ=255)              aTTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGTAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATgg                                                                                                                                  <  5:42875/148‑1 (MQ=255)              aTTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATgg                                                                                                                                  >  1:284505/1‑148 (MQ=255)                  gCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGATTCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCaa                                                                                                                               <  1:95718/148‑1 (MQ=255)                  gCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCa                                                                                                                                >  7:87137/1‑146 (MQ=255)                   cGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAgt                                                                                                                            <  2:178890/149‑2 (MQ=255)                      gggggTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATcg                                                                                                                         <  5:334835/149‑1 (MQ=255)                       ggggTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATcgc                                                                                                                        >  2:272787/1‑149 (MQ=255)                              gCGTACTGCGCACCAGCATTACATTGGCCATGGGCATCTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAgg                                                                                                                 >  6:39700/1‑149 (MQ=255)                                     gcgcACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGc                                                                                                          >  8:339262/1‑149 (MQ=255)                                       gcACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTg                                                                                                        <  6:247101/149‑1 (MQ=255)                                           cAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGgtg                                                                                                     >  1:339772/1‑148 (MQ=255)                                            aGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAACCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTa                                                                                                   <  6:60331/149‑1 (MQ=255)                                                   cATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAAt                                                                                             <  1:304892/148‑1 (MQ=255)                                                     ttGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACg                                                                                          <  2:339772/149‑1 (MQ=255)                                                            tGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATc                                                                                    >  7:249447/1‑148 (MQ=255)                                                             gggCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGa                                                                                  >  4:208072/1‑149 (MQ=255)                                                                 aaCTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTgg                                                                                                       >  6:313732/1‑124 (MQ=255)                                                                  aCTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTg                                                                                                        <  6:380419/122‑1 (MQ=255)                                                                   cagCGGCAGCCCTTCGCTCTCAACCAGAATGCGCATGTTGACCCCGA‑TCAGGCTCTGCAGCTGCGCGCTCACTCCCACCCCGGCGCTGACGATGCCAAAGATCGCCTCCAGGAATGGGCTGGTGTAGGCAATACGGTAATCGATGccat                                                                            >  8:245564/3‑147 (MQ=255)                                                                   cTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCt                                                                            >  4:135737/1‑149 (MQ=255)                                                                    tGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAAt                                                                                     <  8:113508/139‑1 (MQ=255)                                                                    tGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAAt                                                                                     >  7:113508/1‑139 (MQ=255)                                                                      cGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGTGGTCACTGCCAGCCCGGCGTTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGccgt                                                                            <  7:245564/146‑3 (MQ=255)                                                                       ggCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGc                                                                        >  7:149804/1‑149 (MQ=255)                                                                        gCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCt                                                                            <  7:174948/144‑1 (MQ=255)                                                                        gCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCt                                                                            >  8:174948/1‑144 (MQ=255)                                                                          aGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTc                                                                     >  8:226963/1‑149 (MQ=255)                                                                              cTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAgc                                                                 >  2:126930/1‑149 (MQ=255)                                                                               ttCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAgcg                                                                >  3:158923/1‑149 (MQ=255)                                                                                    tctcACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCGCTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTg                                                           >  1:297894/1‑149 (MQ=255)                                                                                      tcACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTg                                                           <  7:339262/147‑1 (MQ=255)                                                                                      tcACCCAGAATGCGGATGTTACCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTg                                                           >  2:313637/1‑147 (MQ=255)                                                                                        aCCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGc                                                          <  3:208072/146‑1 (MQ=255)                                                                                         cccAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGt                                                      >  2:323148/1‑149 (MQ=255)                                                                                          ccAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTc                                                     >  4:41479/1‑149 (MQ=255)                                                                                          ccAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTc                                                     >  5:307273/1‑149 (MQ=255)                                                                                           cAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGGCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTc                                                     >  3:318813/1‑148 (MQ=255)                                                                                                    gATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGAc                                           <  6:307273/149‑1 (MQ=255)                                                                                                        ttGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGaa                                       <  5:39700/149‑1 (MQ=255)                                                                                                           cccccGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCa                                    <  3:316241/149‑1 (MQ=255)                                                                                                             cccGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCGATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGaa                                       <  1:382400/144‑1 (MQ=255)                                                                                                             cccGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCGATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGaa                                       >  2:382400/1‑144 (MQ=255)                                                                                                              ccGA‑TCAGGCTCAGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCGTCTGCCTCCAGCGCATTGCAGGTCCAGGCTCGACAGAAGCAg                                   >  6:322543/1‑147 (MQ=255)                                                                                                               cGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGGCCGGGGGTGACGATGGCAAAGATCGCCGCGAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCTGGCGCGACAGAAGCAGTCg                                <  4:318813/149‑1 (MQ=255)                                                                                                               cGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCGTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCg                                <  1:272787/149‑1 (MQ=255)                                                                                                                 a‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGgt                              <  1:313637/149‑1 (MQ=255)                                                                                                                    cAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGgtgt                            >  2:226310/1‑149 (MQ=255)                                                                                                                     aGGCTCTGCAGCTGCGCGGTCGCTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGgtgt                            <  2:297894/148‑1 (MQ=255)                                                                                                                     aGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGtt                           <  3:41479/149‑1 (MQ=255)                                                                                                                      ggCGCTGCAGCTGCGCGGGCACTGCCAGCCCGGGGGGGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTg                          <  2:284505/149‑1 (MQ=255)                                                                                                                             cAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGaacaac                    <  8:249447/148‑1 (MQ=255)                                                                                                                             cAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACg                   <  8:149804/149‑1 (MQ=255)                                                                                                                                        tCACCGCCGGGCTGGCAGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATc        >  3:364395/1‑149 (MQ=255)                                                                                                                                           cTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATCGGc     <  3:135737/149‑1 (MQ=255)                                                                                                                                            tGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATCGGcc    >  6:157159/1‑149 (MQ=255)                                                                                                                                              ccAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATCGGCCGc  >  3:376417/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 CATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGATGTTGCCCCCGA‑TCAGGCTCTGCAGCTGCGCGGTCACTGCCAGCCCGGCGGTGACGATGGCAAAGATCGCCGCCAGGCTTGGGCTGGTGTAGGCAATACGGTAATCGATGCCCTGTGCCTCCAGCGCATTGCAGGTCCAGGCGCGACAGAAGCAGTCGGTGTTGAACAACGCCACCGGCATCGGCCGC  >  NC_002947/2084155‑2084443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑