Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,132,260 A→G 9.1% L251P (CTG→CCG)  PP_1890 ← type I pili chaperone protein FimC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,132,2600AG 9.1% 120.0 / 2.9 45L251P (CTG→CCG) PP_1890type I pili chaperone protein FimC
Reads supporting (aligned to +/- strand):  ref base A (17/23);  new base G (2/2);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

AGAGCAAGGTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGCTGGCCTTGGCC  >  NC_002947/2132114‑2132408
                                                                                                                                                  |                                                                                                                                                    
agagCAAGGTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAg                                                                                                                                                     <  7:205104/148‑1 (MQ=255)
agagCAAGGTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAg                                                                                                                                                     <  1:304088/148‑1 (MQ=255)
 gagCAAGGTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCg                                                                                                                                                   <  5:22680/149‑1 (MQ=255)
        gTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGt                                                                                                                                            >  3:255753/1‑149 (MQ=255)
           gAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGaa                                                                                                                                         <  7:177962/149‑1 (MQ=255)
           gAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGaa                                                                                                                                         <  5:142717/149‑1 (MQ=255)
                 cccAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCAcc                                                                                                                                   <  5:216945/149‑1 (MQ=255)
                    agcagGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTa                                                                                                                                <  7:294497/149‑1 (MQ=255)
                         gCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGaa                                                                                                                                         >  5:88182/1‑135 (MQ=255)
                         gCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGaa                                                                                                                                         <  6:88182/135‑1 (MQ=255)
                           acaGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTg                                                                                                                         >  6:247123/1‑149 (MQ=255)
                                aaTGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGAt                                                                                                                    <  5:161600/149‑1 (MQ=255)
                                 aTGCGCTGCGCTGGAGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACAATTATGACGATTCCTGTTTGCGTGCGATGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCTGTGAAGCCACCGTAGTCATTGACGATg                                                                                                                   <  6:309645/149‑1 (MQ=255)
                                           ctgGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACggg                                                                                                         <  6:64655/149‑1 (MQ=255)
                                              gcggcgCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGc                                                                                                        <  8:220587/147‑1 (MQ=255)
                                              gcggcgCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCt                                                                                                      >  2:39179/1‑149 (MQ=255)
                                                         tGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGa                                                                                            <  3:135248/148‑1 (MQ=255)
                                                               aGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGgcgc                                                                                       >  2:173675/1‑147 (MQ=255)
                                                               aGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGa                                                                                     <  7:220879/149‑1 (MQ=255)
                                                               aGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGa                                                                                     <  2:53667/149‑1 (MQ=255)
                                                                 cGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGt                                                                                   <  1:39179/149‑1 (MQ=255)
                                                                       gtgtggtggaGCATGATGACGATTGCTTTTTGCGTGCGAGGCAGGGCCTAGTTGGCCAGTTGTATAGTCTCGATCTTCTGTTCGGTGAAGCCACCGTAGTCATTTACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGAGAGTGATGc                                                                              <  2:140220/141‑1 (MQ=255)
                                                                       ggggggAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGcc                                                                             <  1:334804/149‑1 (MQ=255)
                                                                              ggaCCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGc                                                                      <  2:102877/149‑1 (MQ=255)
                                                                              ggaCCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGc                                                                      <  4:34937/149‑1 (MQ=255)
                                                                                  cATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAATACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGt                                                                  <  8:286880/149‑1 (MQ=255)
                                                                                                cTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCGGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGcc                                                     >  3:197577/1‑148 (MQ=255)
                                                                                                cTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCGGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGcc                                                     >  1:203547/1‑148 (MQ=255)
                                                                                                cTGTTTGCGTGCGAGGCAGGCCCTAGTTGGAAAGTTGAATAGTCGCGATCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGcc                                                     >  8:107179/1‑148 (MQ=255)
                                                                                                 tGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCa                                                    >  5:157854/1‑148 (MQ=255)
                                                                                                  gTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCa                                                    >  1:103259/1‑147 (MQ=255)
                                                                                                          gcgAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCGGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCa                                          <  2:203547/149‑1 (MQ=255)
                                                                                                          gcgAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTc                                            >  3:295105/1‑147 (MQ=255)
                                                                                                           cgAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCGGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCa                                          <  4:197577/148‑1 (MQ=255)
                                                                                                                    cccTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGgt                                >  5:242439/1‑149 (MQ=255)
                                                                                                                            ggCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTc                         >  8:347307/1‑148 (MQ=255)
                                                                                                                             gCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCaa                       <  1:173675/149‑1 (MQ=255)
                                                                                                                               cAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGCAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGGTTTTTCGCCATAGGGTTCCAGCATGGCGGTGTTTTTCAAgc                     >  4:154868/1‑148 (MQ=255)
                                                                                                                                  ttGAATAGGCGCGCTCAGCGGTTCGGTGACGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGgtgt                  <  4:255753/149‑1 (MQ=255)
                                                                                                                                  ttGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGgtgt                  <  4:295105/149‑1 (MQ=255)
                                                                                                                                  ttGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGgtgt                  >  3:178360/1‑149 (MQ=255)
                                                                                                                                     aaTAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGtt               >  8:260403/1‑149 (MQ=255)
                                                                                                                                       tAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGc             >  4:366714/1‑149 (MQ=255)
                                                                                                                                       tAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGc             >  4:342/1‑149 (MQ=255)
                                                                                                                                          gcgcgcTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAACGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGCTgg          >  5:90602/1‑149 (MQ=255)
                                                                                                                                                  aGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGCTGGCCTTGGcc  <  7:347307/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGAGCAAGGTGGAGCTACCCAGCAGGCACAGCAATGCGCTGCGCTGGCGGCGCATAGTGCGCCAGCGTAGTGGGGGGAGGACCATGATGACGATTCCTGTTTGCGTGCGAGGCAGGCCCTAGTTGGCCAGTTGAATAGGCGCGCTCAGCGGTTCGGTGAAGCCACCGTAGTCATTGACGATGGAAAAACGGGCCTGCAGGCTGGAGGCGCGAGTGATGCCGGTCAGCGGGTAGGTTTTTTCGCCAAAGGGTTCCAGCATGGCGGTGTTTTTCAAGGTGTGTTGCTGGCCTTGGCC  >  NC_002947/2132114‑2132408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑