Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,144,377 T→G 9.4% H242Q (CAT→CAG)  lpxK → tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,144,3770TG 9.4% 247.4 / 15.3 85H242Q (CAT→CAG) lpxKtetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base T (35/42);  new base G (4/4);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

TGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCAT  >  NC_002947/2144233‑2144520
                                                                                                                                                |                                                                                                                                               
tGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCgg                                                                                                                                             <  6:74342/149‑1 (MQ=255)
    gCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGc                                                                                                                                         >  6:254709/1‑149 (MQ=255)
    gCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGc                                                                                                                                         >  2:298854/1‑149 (MQ=255)
     cTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGcc                                                                                                                                        <  2:266966/149‑1 (MQ=255)
      tGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCg                                                                                                                                              <  4:129960/142‑1 (MQ=255)
      tGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCg                                                                                                                                              >  3:129960/1‑142 (MQ=255)
      tGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCg                                                                                                                                       >  5:385327/1‑149 (MQ=255)
      gatatgggTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCg                                                                                                                                       <  1:333676/143‑1 (MQ=255)
        aCGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCgg                                                                                                                                      >  4:349410/1‑148 (MQ=255)
        aCGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCg                                                                                                                                       <  7:335383/147‑1 (MQ=255)
        aCGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCg                                                                                                                                       <  1:308004/147‑1 (MQ=255)
        aCGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCg                                                                                                                                       <  5:111783/147‑1 (MQ=255)
         cgcgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCggcg                                                                                                                                    <  6:385327/149‑3 (MQ=255)
           cgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCACCGGCCACCGCATGCAGGCGCTGGCCggcggg                                                                                                                                  <  6:302962/149‑5 (MQ=255)
             gTGCTGTTCACCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCgg                                                                                                                                <  3:303895/149‑1 (MQ=255)
                   ttCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAAcc                                                                                                                           <  4:372745/148‑1 (MQ=255)
                      aaCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGc                                                                                                                        >  6:79963/1‑148 (MQ=255)
                        cGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAAc                                                                                                                     >  5:282302/1‑149 (MQ=255)
                            gcCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGttt                                                                                                                 >  6:128552/1‑149 (MQ=255)
                              cAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttct                                                                                                               >  7:303595/1‑149 (MQ=255)
                              cAGCGAAGACCGCACCGATGGTTGCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttct                                                                                                               <  5:79963/149‑1 (MQ=255)
                               aGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttctt                                                                                                              <  3:260090/149‑1 (MQ=255)
                                gCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttcttc                                                                                                             <  5:302066/149‑1 (MQ=255)
                                  gAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCaa                                                                                                           <  6:282302/149‑1 (MQ=255)
                                  gAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCaa                                                                                                           >  5:290702/1‑149 (MQ=255)
                                      aCCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCg                                                                                                                                       <  2:368178/117‑1 (MQ=255)
                                        cGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGttt                                                                                                                 <  2:390073/137‑1 (MQ=255)
                                        cGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCct                                                                                                     >  4:13636/1‑149 (MQ=255)
                                        cGCACCGATGGTTTAGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGttt                                                                                                                 >  1:390073/1‑137 (MQ=255)
                                             cGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgg                                                                                                <  3:349410/149‑1 (MQ=255)
                                               aTGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg                                                                                              <  7:44137/149‑1 (MQ=255)
                                                  gtttcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGAGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCt                                                                                            >  4:261994/1‑148 (MQ=255)
                                                   tttcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTa                                                                                           >  6:338027/1‑148 (MQ=255)
                                                   tttcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTa                                                                                           >  3:133538/1‑148 (MQ=255)
                                                     tcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGGTTCTTCAACACCCTGCTGGGGCTAAAc                                                                                        >  5:258712/1‑149 (MQ=255)
                                                       ggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTg                                                                                      <  5:12228/149‑1 (MQ=255)
                                                        gtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTgg                                                                                     >  7:186868/1‑149 (MQ=255)
                                                        gtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTgg                                                                                     >  6:280351/1‑149 (MQ=255)
                                                              gcctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCg                                                                               >  3:316820/1‑149 (MQ=255)
                                                                ctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAGCACCCTGCTGGGGCTAAACTGGCAGCCGGt                                                                             >  3:333117/1‑149 (MQ=255)
                                                                ctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTgg                                                                                     >  6:153503/1‑141 (MQ=255)
                                                                ctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTgg                                                                                     <  5:153503/141‑1 (MQ=255)
                                                                ctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGt                                                                             >  4:184515/1‑149 (MQ=255)
                                                                   gaGCCGTCGGCCCTGGTCTACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGcc                                                                          <  5:215590/148‑1 (MQ=255)
                                                                   cAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGcc                                                                          <  5:254709/149‑1 (MQ=255)
                                                                   cAGCCGTCGGCCCTGGGCAACCTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTg                                                                            <  3:131331/147‑1 (MQ=255)
                                                                    aGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCg                                                                         <  3:13636/149‑1 (MQ=255)
                                                                       cGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCAt                                                                      <  4:69894/149‑1 (MQ=255)
                                                                       cGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCAt                                                                      >  8:212604/1‑149 (MQ=255)
                                                                       cGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCAt                                                                      <  6:290702/149‑1 (MQ=255)
                                                                               cTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGc                                                              <  8:303595/149‑1 (MQ=255)
                                                                               cTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGc                                                              <  5:338027/149‑1 (MQ=255)
                                                                                  gTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAGCACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGa                                                           <  4:333117/149‑1 (MQ=255)
                                                                                  gTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGa                                                           >  8:345361/1‑149 (MQ=255)
                                                                                  gTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGa                                                           <  1:298854/149‑1 (MQ=255)
                                                                                   tCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGAc                                                          >  5:384342/1‑149 (MQ=255)
                                                                                   tCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGAc                                                          >  2:134167/1‑149 (MQ=255)
                                                                                       cGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCgg                                                                                                                                      >  8:98379/1‑69 (MQ=255)
                                                                                       cGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCgg                                                                                                                                      <  7:98379/69‑1 (MQ=255)
                                                                                            gcACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAg                                                 >  4:212911/1‑149 (MQ=255)
                                                                                             cACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGt                                                >  3:284042/1‑149 (MQ=255)
                                                                                              aCTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                               >  1:168583/1‑149 (MQ=255)
                                                                                                  gCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGt                                           <  3:261994/149‑1 (MQ=255)
                                                                                                  gCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGt                                           <  6:384342/149‑1 (MQ=255)
                                                                                                  gCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCATCGCCGACCATGCGCAGTTCa                                             <  4:316820/147‑1 (MQ=255)
                                                                                                      gcgcCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGccc                                       <  3:212911/149‑1 (MQ=255)
                                                                                                       cgcCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCg                                      <  7:345361/149‑1 (MQ=255)
                                                                                                          cGTGCGCTGGACCATTTCCCCGCCGGCCAGCGCCTGCATGGGGTGGCCGGTATCGGCAACCCTCAACGTTTCTTCAACACGCTGCTGGGGCTATACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGa                                    <  7:172886/148‑1 (MQ=255)
                                                                                                            tGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGcc                                 >  5:386174/1‑149 (MQ=255)
                                                                                                              cgcTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTg                               <  3:184515/149‑1 (MQ=255)
                                                                                                                   gACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCtt                          >  5:113490/1‑149 (MQ=255)
                                                                                                                    aCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTc                         <  5:128552/149‑1 (MQ=255)
                                                                                                                        tttCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCt                                <  4:101634/138‑1 (MQ=255)
                                                                                                                        tttCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCt                                >  3:101634/1‑138 (MQ=255)
                                                                                                                            cccGCCGGCACGCGCTTGCTTGGGGGGGGCGTTATTGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGc                  <  7:239903/148‑1 (MQ=255)
                                                                                                                             ccgccgGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCa                <  6:113490/149‑1 (MQ=255)
                                                                                                                                 cgGCCACCGCATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGc            >  4:75827/1‑149 (MQ=255)
                                                                                                                                   gCCACCGCATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCg          >  4:270833/1‑149 (MQ=255)
                                                                                                                                   gCCACCGCATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCg          >  4:253108/1‑149 (MQ=255)
                                                                                                                                     cAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCt        >  5:119779/1‑149 (MQ=255)
                                                                                                                                         gcacgcctGCGGGGGCCGTGATCCGCAACCCGCAACGTGTCTTCAACACCCTGCTGCGGCTAAACTGGCAGCCGGTGCCGCAGCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTc    <  8:273517/142‑1 (MQ=255)
                                                                                                                                         gcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTTGCCTTCAGCCCGCCACTGCCGCTGGTc    <  5:280351/149‑1 (MQ=255)
                                                                                                                                         gcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTc    <  6:119779/149‑1 (MQ=255)
                                                                                                                                           cTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGc                             <  3:115958/122‑1 (MQ=255)
                                                                                                                                           cTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGc                             >  4:115958/1‑122 (MQ=255)
                                                                                                                                           cTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCAt  >  7:214293/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCAT  >  NC_002947/2144233‑2144520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑