Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,228,274 A→C 9.1% T143P (ACG→CCG) ‡ holB → DNA polymerase III subunit delta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,228,2740AC 9.1% 155.9 / 7.7 55T143P (ACG→CCG) ‡holBDNA polymerase III subunit delta'
Reads supporting (aligned to +/- strand):  ref base A (28/22);  new base C (2/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

TGGCCTTTGTGGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAGCTGCTGAC  >  NC_002947/2228131‑2228419
                                                                                                                                                |                                                                                                                                                 
tggCCTTTGTGGGGAAGACCGCGCAGTTAGGCGGGCGCAAGTTGGTGTTTATCGAGCCTGTGTAGGCGATGAATGTCAACGCCTCAAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGTTGATACCGTGTTGTTGCTGGTGACGCAc                                                                                                                                              <  8:840/149‑1 (MQ=255)
    cTTTGTGGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCAccagc                                                                                                                                          >  3:24664/1‑149 (MQ=255)
    cTTTGTGGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCAccagc                                                                                                                                          <  2:64325/149‑1 (MQ=255)
    cTTTGTGGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCAccagc                                                                                                                                          >  7:174015/1‑149 (MQ=255)
       tgtgGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGCGTCAccagcaa                                                                                                                                        <  5:164188/148‑3 (MQ=255)
       tgtgGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGCGTCAccagcaa                                                                                                                                        <  4:79705/148‑3 (MQ=255)
          ggtgCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACcagcccagcc                                                                                                                                    >  4:307821/1‑149 (MQ=255)
            tgCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGt                                                                                                                                  >  7:86903/1‑149 (MQ=255)
                  ccGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTg                                                                                                                            <  2:287937/149‑1 (MQ=255)
                      gcAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCcga                                                                                                                        <  7:136088/149‑1 (MQ=255)
                       cAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCAc                                                                                                                                              <  3:164368/126‑1 (MQ=255)
                       cAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCAc                                                                                                                                              >  4:164368/1‑126 (MQ=255)
                         gTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCcgacga                                                                                                                     >  4:210601/1‑149 (MQ=255)
                                         gtggtgTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGc                                                                                                       >  3:214937/1‑147 (MQ=255)
                                            gtgtTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCcag                                                                                                    >  3:149154/1‑147 (MQ=255)
                                              gtTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAgg                                                                                                >  8:226318/1‑149 (MQ=255)
                                                 gATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTg                                                                                              <  8:86903/148‑1 (MQ=255)
                                                   tCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGCGTCAccagcaa                                                                                                                                        >  3:140469/1‑102 (MQ=255)
                                                   tCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGCGTCAccagcaa                                                                                                                                        <  4:140469/104‑3 (MQ=255)
                                                        ccGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTcc                                                                                      <  3:307821/149‑1 (MQ=255)
                                                        ccGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTcc                                                                                      >  7:152177/1‑149 (MQ=255)
                                                         cGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTcc                                                                                      <  5:105920/148‑1 (MQ=255)
                                                         cGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTcc                                                                                      >  6:105920/1‑148 (MQ=255)
                                                           gTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCcagcag                                                                                                 >  6:43580/1‑135 (MQ=255)
                                                           gTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCcagcag                                                                                                 <  5:43580/135‑1 (MQ=255)
                                                           gTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCa                                                                                   <  5:358719/149‑1 (MQ=255)
                                                              gAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGcc                                                                                <  7:226318/149‑1 (MQ=255)
                                                               aGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGcc                                                                                >  1:25137/1‑148 (MQ=255)
                                                                   gATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGc                                                                                                                           <  7:63827/101‑1 (MQ=255)
                                                                   gATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGc                                                                                                                           >  8:63827/1‑101 (MQ=255)
                                                                      gAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTg                                                                         <  2:79624/148‑1 (MQ=255)
                                                                       aaTGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTgg                                                                        <  1:179991/148‑1 (MQ=255)
                                                                          gTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGcc                                                                      >  2:256661/1‑147 (MQ=255)
                                                                           tCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCa                                                                    <  4:149154/148‑1 (MQ=255)
                                                                              aCGCCTCCAACGCCCTGCTCAAAAGCGCTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCATGCCTGGctctggg                                                                 >  1:203487/1‑143 (MQ=255)
                                                                              aCGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGc                                                                >  1:262413/1‑149 (MQ=255)
                                                                                  cTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAgg                                                            >  5:355638/1‑149 (MQ=255)
                                                                                  cTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAgg                                                            >  6:217559/1‑149 (MQ=255)
                                                                                     cAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCAt                                                         >  4:263567/1‑149 (MQ=255)
                                                                                           ccTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCATGCCTGGCTCTGGGCCAGACTgggctgc                                                   <  2:203487/149‑2 (MQ=255)
                                                                                            cTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCAtggctggc                                                  >  7:3980/1‑149 (MQ=255)
                                                                                                  aaaaGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTTCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGc                                                                       >  3:75496/1‑122 (MQ=255)
                                                                                                  aaaaGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTACCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGc                                                                       <  4:75496/122‑1 (MQ=255)
                                                                                                         cTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTAcc                                      >  1:189290/1‑149 (MQ=255)
                                                                                                          ttGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTAccc                                     >  7:176421/1‑149 (MQ=255)
                                                                                                                   cTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGcgac                            <  1:256661/149‑1 (MQ=255)
                                                                                                                         gTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTc                      <  3:263567/149‑1 (MQ=255)
                                                                                                                            aTACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGa                    <  8:174015/148‑1 (MQ=255)
                                                                                                                            aTACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAg                   >  7:14768/1‑149 (MQ=255)
                                                                                                                              aCCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAgcg                 >  2:379342/1‑149 (MQ=255)
                                                                                                                               ccGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAgcgc                <  2:25137/149‑1 (MQ=255)
                                                                                                                                       gggCTGGTGCGTCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAgct        >  4:59144/3‑149 (MQ=255)
                                                                                                                                         gCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAgctgc      >  3:61573/1‑149 (MQ=255)
                                                                                                                                          cTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAgctgc      <  1:379342/148‑1 (MQ=255)
                                                                                                                                             gTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAGCTGCTGAc  >  8:304913/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGGCCTTTGTGGTGCAGACCGCGCAGTTAGGCGGGCGCAAGGTGGTGTTGATCGAGCCGGTGGAGGCGATGAATGTCAACGCCTCCAACGCCCTGCTCAAAAGC‑CTTGAAGAACCTTCCGGTGATACCGTGTTGTTGCTGGTGACGCACCAGCCCAGCCGTCTGTTGCCGACGATCAAGAGCCGCTGCCAGCAGGTGGCCTGTCCGCAGCCCAGTCTGGCCCAGAGCCAGGCATGGCTGGCTGGCGCGTTACCCGACTGCGACGAGGTCGAGCGCGATGAGCTGCTGAC  >  NC_002947/2228131‑2228419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑