Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,266,715	C→G	7.7%	R161G (CGT→GGT) ‡	dedD →	cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,266,715	0	C	G	7.7%	191.8 / 6.7	65	R161G (CGT→GGT) ‡	dedD	cell division protein
Reads supporting (aligned to +/- strand):  ref base C (36/24);  new base G (3/2);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

CGCGCCCAAGGTGGAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTC  >  NC_002947/2266567‑2266861                                                                                                                                                     |                                                                                                                                                   cgcgcccAAGGTGGAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGc                                                                                                                                                    <  3:317431/149‑1 (MQ=255)           gTGGAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAggg                                                                                                                                          <  2:237714/149‑1 (MQ=255)            tGGAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGt                                                                                                                                         <  1:84475/149‑1 (MQ=255)              gAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTa                                                                                                                                       >  5:30045/1‑149 (MQ=255)               aGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGGCTACGCAGGGtttg                                                                                                                                      <  8:78951/149‑3 (MQ=255)                    aaaGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGGCTACGCAGGGtttgctgca                                                                                                                                 <  1:283543/149‑8 (MQ=255)                    aaaGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGc                                                                                                                                 >  1:117571/1‑149 (MQ=255)                              cAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCg                                                                                                                        <  1:160230/148‑1 (MQ=255)                              cAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCg                                                                                                                        >  4:14878/1‑148 (MQ=255)                               aaCACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTa                                                                                                                      >  7:102304/1‑149 (MQ=255)                                acacCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTAc                                                                                                                     >  2:372451/1‑149 (MQ=255)                                 cacCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTAcg                                                                                                                    >  8:14126/1‑149 (MQ=255)                                 cacCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATACAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTCCAGCAAACCCTGCGAAGCCAGGGTTACAACGCCTATATCCGTAcg                                                                                                                    >  6:95719/1‑149 (MQ=255)                                    cAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTAcggcg                                                                                                                 >  6:208990/1‑149 (MQ=255)                                    cAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTAcggcg                                                                                                                 >  8:59638/1‑149 (MQ=255)                                    cAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTAcggcg                                                                                                                 >  2:87120/1‑149 (MQ=255)                                         ccTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTgg                                                                                                            <  5:208990/149‑1 (MQ=255)                                          cTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCggg                                                                                                               <  7:253400/145‑1 (MQ=255)                                          cTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCggg                                                                                                               >  8:253400/1‑145 (MQ=255)                                             ccGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCAt                                                                                                         >  6:62712/1‑148 (MQ=255)                                                     tCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGt                                                                                                <  4:74731/149‑1 (MQ=255)                                                     tCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGt                                                                                                >  3:253691/1‑149 (MQ=255)                                                        aaGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTa                                                                                             >  1:349732/1‑149 (MQ=255)                                                         aGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTAc                                                                                            <  8:102304/149‑1 (MQ=255)                                                           aTCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGt                                                                                          <  7:14126/149‑1 (MQ=255)                                                            tCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAATCCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTg                                                                                         <  5:95719/149‑1 (MQ=255)                                                              gACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTgg                                                                                        <  2:349732/148‑1 (MQ=255)                                                                   cAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGt                                                                                  <  3:14878/149‑1 (MQ=255)                                                                      tGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGa                                                                               <  2:117571/149‑1 (MQ=255)                                                                         ccTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATc                                                                             >  2:66027/1‑148 (MQ=255)                                                                         ccTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCg                                                                            <  5:62712/149‑1 (MQ=255)                                                                               cGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAgcgcg                                                                      >  3:370051/1‑149 (MQ=255)                                                                                    gTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCgccgag                                                                 >  4:250465/1‑149 (MQ=255)                                                                                     ttGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCgccgagg                                                                >  1:33299/1‑149 (MQ=255)                                                                                           gATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAgc                                                          >  3:104711/1‑149 (MQ=255)                                                                                           gATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAgc                                                          >  2:19322/1‑149 (MQ=255)                                                                                               cAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCgcgt                                                      <  8:222729/149‑1 (MQ=255)                                                                                                aGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGa                                                                               <  2:242159/123‑1 (MQ=255)                                                                                                aGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGa                                                                               >  1:242159/1‑123 (MQ=255)                                                                                                 gCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAgc                                                          <  4:236344/143‑1 (MQ=255)                                                                                                 gCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAgc                                                          >  3:236344/1‑143 (MQ=255)                                                                                                 gCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCgcgtgc                                                    <  6:30045/149‑1 (MQ=255)                                                                                                     gCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGa                                                >  2:48841/1‑149 (MQ=255)                                                                                                       cAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGtt                                                                                 >  1:361164/1‑114 (MQ=255)                                                                                                       cAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGtt                                                                                 <  2:361164/114‑1 (MQ=255)                                                                                                        aGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGc                                             <  1:372451/149‑1 (MQ=255)                                                                                                              tCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATc                                         <  2:187202/147‑1 (MQ=255)                                                                                                              tCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGAGACGAGGCCGAGCGCGTGCGTGACGCCATCaa                                       >  8:179206/1‑149 (MQ=255)                                                                                                                cAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAAcc                                     <  4:104711/149‑1 (MQ=255)                                                                                                                cAACCGCGCACGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGACCGAGCGCGTGCGTGACGCCATCAAcc                                     >  5:88590/1‑149 (MQ=255)                                                                                                                 aacaccgcAGGAGCCGAAAAGCTGCACCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTATACGAGGGGACGTTGATCGAGCGCCACGACGCCGAGCGCGTGCGTGACGCCAACAACCg                                    >  2:269264/6‑149 (MQ=255)                                                                                                                 aaCCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCg                                    >  8:329555/1‑149 (MQ=255)                                                                                                                           gAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGCCGCCATCAACCGTCAGAACAGc                          <  7:59638/149‑1 (MQ=255)                                                                                                                               cGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTc                       >  4:155426/1‑148 (MQ=255)                                                                                                                               cGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTc                       >  8:343371/1‑148 (MQ=255)                                                                                                                                gAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTc                       >  1:173523/1‑147 (MQ=255)                                                                                                                                 aaaaGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAg                    >  7:326696/1‑149 (MQ=255)                                                                                                                                         gttgtaaCCCTGGCTACGCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCgt            >  5:152713/6‑149 (MQ=255)                                                                                                                                            gtaaCCCTGGCTACGCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGGCTCAAGGGCTTCgtggt         >  2:356003/3‑149 (MQ=255)                                                                                                                                            caAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCgtggt         >  6:47440/1‑149 (MQ=255)                                                                                                                                            caAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCgtggt         <  1:66027/149‑1 (MQ=255)                                                                                                                                             taaCCCTGGCTACGCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCgtggtg        >  4:64564/2‑149 (MQ=255)                                                                                                                                             aaaCCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCgtggtg        <  4:253691/149‑1 (MQ=255)                                                                                                                                                 ccTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCt    >  7:313456/1‑149 (MQ=255)                                                                                                                                                 ccTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCt    >  6:86254/1‑149 (MQ=255)                                                                                                                                                   tGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTc  >  2:278215/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGCGCCCAAGGTGGAGGCCAAAGCTGCTGCAACACCAGCACCTGCCGCGCCATCGAAGATCGACGCCAATGGCCTGCCCGTCAGTTGGTCGATCCAGCTGGCCAGTCTGTCCAACCGCGCAGGAGCCGAAAAGCTGCAGCAAACCCTGCGTAGCCAGGGTTACAACGCCTATATCCGTACGGCGGGTGGCATGAACCGCGTTTACGTGGGGCCGTTGATCGAGCGCGCCGAGGCCGAGCGCGTGCGTGACGCCATCAACCGTCAGAACAGCCTCAAGGGCTTCGTGGTGCGCTTC  >  NC_002947/2266567‑2266861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑