Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,628 A→G 6.4% T424A (ACA→GCA)  dctA‑II → C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,6280AG 6.4% 184.4 / 2.0 62T424A (ACA→GCA) dctA‑IIC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base G (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CCGCTTCATGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAC  >  NC_002947/2340480‑2340775
                                                                                                                                                     |                                                                                                                                                   
ccGCTTCATGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGaaa                                                                                                                                                     <  6:203660/149‑1 (MQ=255)
  gCTTCATGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACa                                                                                                                                                   <  5:85852/149‑1 (MQ=255)
     tCATGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGcc                                                                                                                                                >  3:83166/1‑149 (MQ=255)
       aTGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGACCGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGcc                                                                                                                                                <  1:106510/148‑1 (MQ=255)
       aTGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGa                                                                                                                                              <  7:5027/149‑1 (MQ=255)
            ggAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGCAACAGCCGACTggg                                                                                                                                         >  5:385803/1‑149 (MQ=255)
            ggAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTggg                                                                                                                                         >  7:55258/1‑149 (MQ=255)
                 cccGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGcc                                                                                                                                    >  1:128859/1‑149 (MQ=255)
                       cgcTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAAcc                                                                                                                               <  3:28643/148‑1 (MQ=255)
                          tGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCacac                                                                                                                           >  4:143114/1‑149 (MQ=255)
                             cGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGc                                                                                                                        >  3:129442/1‑149 (MQ=255)
                              gAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGcc                                                                                                                       >  2:138191/1‑149 (MQ=255)
                                        aGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGcc                                                                                                                                    >  7:11475/1‑126 (MQ=255)
                                        aGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGcc                                                                                                                                    <  8:11475/126‑1 (MQ=255)
                                              gCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGac                                                                                                       <  7:340827/149‑1 (MQ=255)
                                                aGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGacac                                                                                                     <  8:67807/149‑1 (MQ=255)
                                                    gCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCt                                                                                                  <  5:175133/148‑1 (MQ=255)
                                                    gCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCt                                                                                                  <  2:128859/148‑1 (MQ=255)
                                                      gACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCaa                                                                                               <  5:34375/149‑1 (MQ=255)
                                                           tggtggtTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCttt                                                                                          <  8:140005/149‑1 (MQ=255)
                                                             gtggtTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCttttt                                                                                        <  3:162573/149‑1 (MQ=255)
                                                                gtTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCt                                                                                                                 >  4:177556/1‑121 (MQ=255)
                                                                gtTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCt                                                                                                                 <  3:177556/121‑1 (MQ=255)
                                                                  ttCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAgg                                                                                   >  8:208773/1‑149 (MQ=255)
                                                                             aaaaCGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                        >  8:227009/1‑149 (MQ=255)
                                                                              aaaCGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                        <  1:138191/148‑1 (MQ=255)
                                                                                    cTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                 >  7:53708/1‑149 (MQ=255)
                                                                                    cTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                 >  3:99088/1‑149 (MQ=255)
                                                                                    cTGTGACCGCCAGA‑CGCTGCACAGACGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                 >  7:58438/1‑149 (MQ=255)
                                                                                       tgACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCgg                                                               <  4:129442/148‑1 (MQ=255)
                                                                                        gACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGt                                                             >  4:353397/1‑149 (MQ=255)
                                                                                            gCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                         <  8:55258/149‑1 (MQ=255)
                                                                                            gCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTgggg                                                         <  3:143114/149‑1 (MQ=255)
                                                                                            gCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGGCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCCCGGCTGAGCCCCGACACCCTCCAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                         <  6:385803/149‑1 (MQ=255)
                                                                                             ccAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGCCACCCTCAAGCTTGTTGCAGGCCCCCACCAGGCGTGGCCGgggggggg                                                        >  8:271175/1‑149 (MQ=255)
                                                                                                    gCTGCACAGCCGGCTGACCGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                  <  7:305921/149‑1 (MQ=255)
                                                                                                    gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                  <  8:151632/149‑1 (MQ=255)
                                                                                                    gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                  >  1:77158/1‑149 (MQ=255)
                                                                                                      tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                >  8:92349/1‑149 (MQ=255)
                                                                                                      tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTAGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                >  1:110876/1‑149 (MQ=255)
                                                                                                        cacaGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGcc                                              >  3:158733/1‑149 (MQ=255)
                                                                                                          caGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGt                                            <  3:353397/149‑1 (MQ=255)
                                                                                                             ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTg                                         <  2:110876/149‑1 (MQ=255)
                                                                                                             ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCG‑CACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgc                                        <  8:36731/149‑1 (MQ=255)
                                                                                                                        gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                              >  5:373384/1‑149 (MQ=255)
                                                                                                                        gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                              <  7:92349/149‑1 (MQ=255)
                                                                                                                         cGAGCCGGTGGTCACCCCCGTCGCTTAAACAGCCGACTGGGTCGCAGAACCACACCGCCCCGGCAGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGGGGGGGTCAGGGGGCCGTCTCCGAGGCAGATgg                             >  8:345648/1‑149 (MQ=255)
                                                                                                                         cGAGCCGGTGGTCACCCCCGGCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTgg                             <  7:271175/149‑1 (MQ=255)
                                                                                                                            gCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCca                          >  3:204928/1‑149 (MQ=255)
                                                                                                                             ccgctcttCACCCCCGGCCCTGAAACACCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGTCCCGTCTGCGCTGCAGGTGGCca                          <  7:58834/141‑1 (MQ=255)
                                                                                                                                gtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCcaca                        >  1:263190/1‑147 (MQ=255)
                                                                                                                                    tCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                   >  8:164196/1‑148 (MQ=255)
                                                                                                                                      aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                 >  8:297606/1‑148 (MQ=255)
                                                                                                                                      aCCCCCGTCGCTGAAACAGCCGACTGGGGCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                 <  4:83166/148‑1 (MQ=255)
                                                                                                                                       cccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa               <  7:227009/149‑1 (MQ=255)
                                                                                                                                        ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa               >  6:42499/1‑148 (MQ=255)
                                                                                                                                         cccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaa             <  4:204928/149‑1 (MQ=255)
                                                                                                                                          ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                <  4:174506/113‑1 (MQ=255)
                                                                                                                                          ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                >  3:174506/1‑113 (MQ=255)
                                                                                                                                          ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaa            <  2:77158/149‑1 (MQ=255)
                                                                                                                                           cGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaaa           <  5:136406/149‑1 (MQ=255)
                                                                                                                                                cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCATGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga      <  8:53708/149‑1 (MQ=255)
                                                                                                                                                cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga      <  8:58438/149‑1 (MQ=255)
                                                                                                                                                   aaaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa   <  7:208773/149‑1 (MQ=255)
                                                                                                                                                     aCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAc  >  3:332122/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGCTTCATGTCGGAAGCCCGTGCGCTGACGAGTCTTGCCAGCAACGCAGTGGCGACGGTGGTGGTTTCGTTGTCGGAAAACGCCTGTGACCGCCAGA‑CGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAC  >  NC_002947/2340480‑2340775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑