Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,703 A→C 6.0% intergenic (+37/‑78) dctA‑II → / → yraO C4‑dicarboxylate transport protein/transporter YraO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,7030AC 6.0% 194.5 / 4.1 67intergenic (+37/‑78)dctA‑II/yraOC4‑dicarboxylate transport protein/transporter YraO
Reads supporting (aligned to +/- strand):  ref base A (31/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

AAAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCA  >  NC_002947/2340557‑2340849
                                                                                                                                                  |                                                                                                                                                  
aaaaCGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                  >  8:227009/1‑149 (MQ=255)
 aaaCGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAgg                                                                                                                                                  <  1:138191/148‑1 (MQ=255)
       cTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                                                                                           >  7:53708/1‑149 (MQ=255)
       cTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                                                                                           >  3:99088/1‑149 (MQ=255)
       cTGTGACCGCCAGACGCTGCACAGACGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGcc                                                                                                                                           >  7:58438/1‑149 (MQ=255)
          tgACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCgg                                                                                                                                         <  4:129442/148‑1 (MQ=255)
           gACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGt                                                                                                                                       >  4:353397/1‑149 (MQ=255)
               gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                                                                                                   <  8:55258/149‑1 (MQ=255)
               gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTgggg                                                                                                                                   <  3:143114/149‑1 (MQ=255)
               gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGGCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCCCGGCTGAGCCCCGACACCCTCCAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                                                                                                   <  6:385803/149‑1 (MQ=255)
                ccAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGCCACCCTCAAGCTTGTTGCAGGCCCCCACCAGGCGTGGCCGgggggggg                                                                                                                                  >  8:271175/1‑149 (MQ=255)
                      gCTGCACAGCCGGCTGACCGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            <  7:305921/149‑1 (MQ=255)
                      gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            >  1:77158/1‑149 (MQ=255)
                      gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            <  8:151632/149‑1 (MQ=255)
                        tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  8:92349/1‑149 (MQ=255)
                        tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTAGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  1:110876/1‑149 (MQ=255)
                          cacaGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGcc                                                                                                                        >  3:158733/1‑149 (MQ=255)
                            caGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGt                                                                                                                      <  3:353397/149‑1 (MQ=255)
                               ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTg                                                                                                                   <  2:110876/149‑1 (MQ=255)
                               ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCG‑CACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgc                                                                                                                  <  8:36731/149‑1 (MQ=255)
                                          gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                        <  7:92349/149‑1 (MQ=255)
                                          gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                        >  5:373384/1‑149 (MQ=255)
                                           cGAGCCGGTGGTCACCCCCGTCGCTTAAACAGCCGACTGGGTCGCAGAACCACACCGCCCCGGCAGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGGGGGGGTCAGGGGGCCGTCTCCGAGGCAGATgg                                                                                                       >  8:345648/1‑149 (MQ=255)
                                           cGAGCCGGTGGTCACCCCCGGCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTgg                                                                                                       <  7:271175/149‑1 (MQ=255)
                                              gCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCca                                                                                                    >  3:204928/1‑149 (MQ=255)
                                               ccgctcttCACCCCCGGCCCTGAAACACCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGTCCCGTCTGCGCTGCAGGTGGCca                                                                                                    <  7:58834/141‑1 (MQ=255)
                                                  gtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCcaca                                                                                                  >  1:263190/1‑147 (MQ=255)
                                                      tCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                             >  8:164196/1‑148 (MQ=255)
                                                        aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                           >  8:297606/1‑148 (MQ=255)
                                                        aCCCCCGTCGCTGAAACAGCCGACTGGGGCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                           <  4:83166/148‑1 (MQ=255)
                                                         cccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa                                                                                         <  7:227009/149‑1 (MQ=255)
                                                          ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa                                                                                         >  6:42499/1‑148 (MQ=255)
                                                           cccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaa                                                                                       <  4:204928/149‑1 (MQ=255)
                                                            ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  3:174506/1‑113 (MQ=255)
                                                            ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          <  4:174506/113‑1 (MQ=255)
                                                            ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaa                                                                                      <  2:77158/149‑1 (MQ=255)
                                                             cGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaaa                                                                                     <  5:136406/149‑1 (MQ=255)
                                                                  cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCATGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                <  8:53708/149‑1 (MQ=255)
                                                                  cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                <  8:58438/149‑1 (MQ=255)
                                                                     aaaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                             <  7:208773/149‑1 (MQ=255)
                                                                       aCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAc                                                                            >  3:332122/1‑148 (MQ=255)
                                                                           ccGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAAc                                                                        >  5:116726/1‑148 (MQ=255)
                                                                            cGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCa                                                                      >  1:106141/1‑149 (MQ=255)
                                                                             gACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCAt                                                                     >  6:339297/1‑149 (MQ=255)
                                                                                    tCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCa                                                              >  5:172573/1‑149 (MQ=255)
                                                                                        cGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTc                                                          >  5:140/1‑149 (MQ=255)
                                                                                          aaCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTg                                                        <  2:106141/149‑1 (MQ=255)
                                                                                           aCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTgg                                                       <  6:172573/149‑1 (MQ=255)
                                                                                            ccACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTggg                                                      >  2:340287/1‑149 (MQ=255)
                                                                                             cacaCCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                     <  6:140/149‑1 (MQ=255)
                                                                                              acacCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGcc                                                    >  1:229974/1‑149 (MQ=255)
                                                                                                       cGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtgg                                           <  2:229974/149‑1 (MQ=255)
                                                                                                             aGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGgtggtg                                      <  5:42499/148‑1 (MQ=255)
                                                                                                                      acCCTCAAGCTTTTTGCAGGCCCGCACGAGGCGTTGCCGGTTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGATAAGAAACGATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacc                             <  7:345648/148‑1 (MQ=255)
                                                                                                                      acCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacct                            <  5:339297/149‑1 (MQ=255)
                                                                                                                         cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         <  6:373384/149‑1 (MQ=255)
                                                                                                                         cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         <  4:332122/149‑1 (MQ=255)
                                                                                                                         cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGCGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         >  2:271487/1‑149 (MQ=255)
                                                                                                                          tCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacct                        >  4:333343/1‑149 (MQ=255)
                                                                                                                          tCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTCGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGCTGGTGACCTTCacctacct                        >  3:295350/1‑149 (MQ=255)
                                                                                                                              gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATc                    <  6:116726/149‑1 (MQ=255)
                                                                                                                                   ttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGa                <  4:99088/148‑1 (MQ=255)
                                                                                                                                      cAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCt                                                   >  3:147719/1‑110 (MQ=255)
                                                                                                                                      cAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCt                                                   <  4:147719/110‑1 (MQ=255)
                                                                                                                                           ccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc       >  5:335006/1‑149 (MQ=255)
                                                                                                                                           ccccACCCGGCAACGCCTGGTGGGGGCCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc       >  6:142539/1‑149 (MQ=255)
                                                                                                                                            cccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt      >  5:278015/1‑149 (MQ=255)
                                                                                                                                             ccaccaGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTg     >  6:130213/1‑149 (MQ=255)
                                                                                                                                                ccaGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCa  <  6:278015/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCA  >  NC_002947/2340557‑2340849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑