Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,713 G→T 6.4% intergenic (+47/‑68) dctA‑II → / → yraO C4‑dicarboxylate transport protein/transporter YraO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,7130GT 6.4% 196.5 / 3.7 63intergenic (+47/‑68)dctA‑II/yraOC4‑dicarboxylate transport protein/transporter YraO
Reads supporting (aligned to +/- strand):  ref base G (28/31);  new base T (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

TGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCA  >  NC_002947/2340567‑2340849
                                                                                                                                                  |                                                                                                                                        
tgACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCgg                                                                                                                                         <  4:129442/148‑1 (MQ=255)
 gACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGt                                                                                                                                       >  4:353397/1‑149 (MQ=255)
     gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                                                                                                   <  8:55258/149‑1 (MQ=255)
     gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTgggg                                                                                                                                   <  3:143114/149‑1 (MQ=255)
     gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGGCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCCCGGCTGAGCCCCGACACCCTCCAGCTTTTTGCAGGCCCCCACCCGGCAACGCCTGGTgggg                                                                                                                                   <  6:385803/149‑1 (MQ=255)
      ccAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGCCACCCTCAAGCTTGTTGCAGGCCCCCACCAGGCGTGGCCGgggggggg                                                                                                                                  >  8:271175/1‑149 (MQ=255)
            gCTGCACAGCCGGCTGACCGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            <  7:305921/149‑1 (MQ=255)
            gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            >  1:77158/1‑149 (MQ=255)
            gCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggg                                                                                                                            <  8:151632/149‑1 (MQ=255)
              tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  8:92349/1‑149 (MQ=255)
              tGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTAGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  1:110876/1‑149 (MQ=255)
                cacaGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGcc                                                                                                                        >  3:158733/1‑149 (MQ=255)
                  caGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGt                                                                                                                      <  3:353397/149‑1 (MQ=255)
                     ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTg                                                                                                                   <  2:110876/149‑1 (MQ=255)
                     ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCG‑CACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTgc                                                                                                                  <  8:36731/149‑1 (MQ=255)
                                gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                        <  7:92349/149‑1 (MQ=255)
                                gCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                        >  5:373384/1‑149 (MQ=255)
                                 cGAGCCGGTGGTCACCCCCGTCGCTTAAACAGCCGACTGGGTCGCAGAACCACACCGCCCCGGCAGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGGGGGGGTCAGGGGGCCGTCTCCGAGGCAGATgg                                                                                                       >  8:345648/1‑149 (MQ=255)
                                 cGAGCCGGTGGTCACCCCCGGCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTgg                                                                                                       <  7:271175/149‑1 (MQ=255)
                                    gCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCca                                                                                                    >  3:204928/1‑149 (MQ=255)
                                     ccgctcttCACCCCCGGCCCTGAAACACCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGTCCCGTCTGCGCTGCAGGTGGCca                                                                                                    <  7:58834/141‑1 (MQ=255)
                                        gtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCcaca                                                                                                  >  1:263190/1‑147 (MQ=255)
                                            tCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                             >  8:164196/1‑148 (MQ=255)
                                              aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                           >  8:297606/1‑148 (MQ=255)
                                              aCCCCCGTCGCTGAAACAGCCGACTGGGGCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACaa                                                                                           <  4:83166/148‑1 (MQ=255)
                                               cccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa                                                                                         <  7:227009/149‑1 (MQ=255)
                                                ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATa                                                                                         >  6:42499/1‑148 (MQ=255)
                                                 cccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaa                                                                                       <  4:204928/149‑1 (MQ=255)
                                                  ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          >  3:174506/1‑113 (MQ=255)
                                                  ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAggggg                                                                                                                          <  4:174506/113‑1 (MQ=255)
                                                  ccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaa                                                                                      <  2:77158/149‑1 (MQ=255)
                                                   cGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATaaaaa                                                                                     <  5:136406/149‑1 (MQ=255)
                                                        cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCATGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                <  8:53708/149‑1 (MQ=255)
                                                        cTGAAGCAACCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                <  8:58438/149‑1 (MQ=255)
                                                           aaaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                             <  7:208773/149‑1 (MQ=255)
                                                             aCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAAc                                                                            >  3:332122/1‑148 (MQ=255)
                                                                 ccGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAAc                                                                        >  5:116726/1‑148 (MQ=255)
                                                                  cGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCa                                                                      >  1:106141/1‑149 (MQ=255)
                                                                   gACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCAt                                                                     >  6:339297/1‑149 (MQ=255)
                                                                          tCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCa                                                              >  5:172573/1‑149 (MQ=255)
                                                                              cGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTc                                                          >  5:140/1‑149 (MQ=255)
                                                                                aaCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTg                                                        <  2:106141/149‑1 (MQ=255)
                                                                                 aCCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTgg                                                       <  6:172573/149‑1 (MQ=255)
                                                                                  ccACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTggg                                                      >  2:340287/1‑149 (MQ=255)
                                                                                   cacaCCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                     <  6:140/149‑1 (MQ=255)
                                                                                    acacCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGcc                                                    >  1:229974/1‑149 (MQ=255)
                                                                                             cGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAtgg                                           <  2:229974/149‑1 (MQ=255)
                                                                                                   aGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGgtggtg                                      <  5:42499/148‑1 (MQ=255)
                                                                                                            acCCTCAAGCTTTTTGCAGGCCCGCACGAGGCGTTGCCGGTTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGATAAGAAACGATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacc                             <  7:345648/148‑1 (MQ=255)
                                                                                                            acCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacct                            <  5:339297/149‑1 (MQ=255)
                                                                                                               cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         <  6:373384/149‑1 (MQ=255)
                                                                                                               cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         <  4:332122/149‑1 (MQ=255)
                                                                                                               cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGCGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacc                         >  2:271487/1‑149 (MQ=255)
                                                                                                                tCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCacctacct                        >  4:333343/1‑149 (MQ=255)
                                                                                                                tCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTCGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGCTGGTGACCTTCacctacct                        >  3:295350/1‑149 (MQ=255)
                                                                                                                    gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATc                    <  6:116726/149‑1 (MQ=255)
                                                                                                                         ttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGa                <  4:99088/148‑1 (MQ=255)
                                                                                                                            cAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCt                                                   <  4:147719/110‑1 (MQ=255)
                                                                                                                            cAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCt                                                   >  3:147719/1‑110 (MQ=255)
                                                                                                                                 ccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc       >  5:335006/1‑149 (MQ=255)
                                                                                                                                 ccccACCCGGCAACGCCTGGTGGGGGCCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc       >  6:142539/1‑149 (MQ=255)
                                                                                                                                  cccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt      >  5:278015/1‑149 (MQ=255)
                                                                                                                                   ccaccaGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTg     >  6:130213/1‑149 (MQ=255)
                                                                                                                                      ccaGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCa  <  6:278015/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
TGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCA  >  NC_002947/2340567‑2340849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑