Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,377,402 G→A 7.5% A15T (GCC→ACC)  cmaX → protein CmaX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,377,4020GA 7.5% 149.3 / 2.4 54A15T (GCC→ACC) cmaXprotein CmaX
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

CTTTCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTGGCTGCTGCACGACAGCGGCCT  >  NC_002947/2377256‑2377550
                                                                                                                                                   |                                                                                                                                                    
cTTTCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGcc                                                                                                                                                    <  6:95032/149‑1 (MQ=255)
 tttCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGccc                                                                                                                                                   >  8:160187/1‑149 (MQ=255)
 tttCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGccc                                                                                                                                                   >  2:126705/1‑149 (MQ=255)
 tttCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGccc                                                                                                                                                   >  2:225931/1‑149 (MQ=255)
 tttCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGccc                                                                                                                                                   >  3:325984/1‑149 (MQ=255)
    ccGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTg                                                                                                                                                 <  6:137169/148‑1 (MQ=255)
    ccGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTg                                                                                                                                                 <  5:93301/148‑1 (MQ=255)
     cGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGATTAATGCGCCGCACGCGCTGATGGAGCTTTTATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTgg                                                                                                                                                <  7:15615/148‑1 (MQ=255)
     cGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTgg                                                                                                                                                <  8:95035/148‑1 (MQ=255)
      gCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGCAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTGTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCGTGCCGTgggg                                                                                                                                              >  4:270116/1‑147 (MQ=255)
               aGTATGGTTATGCCGACAACATTGGCGTGGTGGTCTCGCCTAAACCGCTGAAAATTGCCGGCTTAATGCGCCGCACGCGCTGATGGTGCTTTGTTGTTCGAGGAAGAAAACGCGCAGTTGGGGCTGGTGCTTGCCGTGGTGCTCGATg                                                                                                                                       <  4:196309/148‑1 (MQ=255)
                   tgtgTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAg                                                                                                                                 >  3:11519/1‑149 (MQ=255)
                   tgtgTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAACACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAg                                                                                                                                 <  3:105300/149‑1 (MQ=255)
                     tgtATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAgg                                                                                                                                <  7:160187/148‑1 (MQ=255)
                           ccGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGgcgcg                                                                                                                         <  1:225931/149‑1 (MQ=255)
                              ccaacaaTGGCGTGGTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCGCGCTGGCACAGGGGgcgcgcgc                                                                                                                      >  4:266176/2‑149 (MQ=255)
                                aacaaTGGCGTGCTGGTCTCGCCGAAACGGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTc                                                                                                                    >  1:217137/1‑149 (MQ=255)
                                  caaTGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGa                                                                                                                  >  3:183115/1‑149 (MQ=255)
                                    aTGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTACTGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGAtt                                                                                                                <  1:387818/149‑1 (MQ=255)
                                         gTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGAATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCg                                                                                                           >  3:244343/1‑149 (MQ=255)
                                              ggTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGa                                                                                                                                         <  3:258382/114‑1 (MQ=255)
                                              ggTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGa                                                                                                                                         >  4:258382/1‑114 (MQ=255)
                                               gTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCg                                                                                                      >  5:173639/1‑148 (MQ=255)
                                                    gCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACt                                                                                                  <  2:217137/147‑1 (MQ=255)
                                                               tGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAg                                                                                     >  2:376195/1‑149 (MQ=255)
                                                                   aa‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGc                                                                                 <  1:376195/149‑1 (MQ=255)
                                                                       gCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAAc                                                                              >  5:359406/1‑149 (MQ=255)
                                                                           gAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGa                                                                          <  4:11519/149‑1 (MQ=255)
                                                                               aaTGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCaa                                                                                                                                   <  8:154009/88‑1 (MQ=255)
                                                                               aaTGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCaa                                                                                                                                   >  7:154009/1‑88 (MQ=255)
                                                                                 tGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATACCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCagag                                                                    >  3:211847/1‑149 (MQ=255)
                                                                                 tGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATACCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCagag                                                                    >  3:216713/1‑149 (MQ=255)
                                                                                   cgccgcACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGc                                                                   <  4:325984/148‑1 (MQ=255)
                                                                                     ccgcACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCtg                                                                >  2:348068/1‑149 (MQ=255)
                                                                                            cgcTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATACCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGc                                                         <  4:216713/149‑1 (MQ=255)
                                                                                            cgcTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATACCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGc                                                         <  4:211847/149‑1 (MQ=255)
                                                                                                 aTGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACt                                                      <  6:173639/147‑1 (MQ=255)
                                                                                                      gCTTTGATGTTCGAGGTAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCg                                               >  4:304863/1‑149 (MQ=255)
                                                                                                      gCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCg                                               <  4:183115/149‑1 (MQ=255)
                                                                                                          tGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGc                                           >  7:155576/1‑149 (MQ=255)
                                                                                                               ttCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATcc                                      >  5:163697/1‑149 (MQ=255)
                                                                                                               ttCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATcc                                      <  2:3051/149‑1 (MQ=255)
                                                                                                                tCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATcc                                      <  2:82976/148‑1 (MQ=255)
                                                                                                                tCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATcc                                      >  1:82976/1‑148 (MQ=255)
                                                                                                                   aGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCa                                   >  8:149788/1‑148 (MQ=255)
                                                                                                                     gAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAAc                                >  7:70269/1‑149 (MQ=255)
                                                                                                                     gAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAAc                                >  2:97595/1‑149 (MQ=255)
                                                                                                                     gAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAAc                                <  8:155576/149‑1 (MQ=255)
                                                                                                                        gACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCg                             >  5:81192/1‑149 (MQ=255)
                                                                                                                              gcgcAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTg                       <  1:97595/149‑1 (MQ=255)
                                                                                                                              gcgcAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTg                       <  1:348068/149‑1 (MQ=255)
                                                                                                                                gcAGTGGGGGCTGGTGAATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGAGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGCGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTgcc                     >  2:330379/1‑147 (MQ=255)
                                                                                                                                                cATTCCCTGGTGCTCGATGGGAAAGGTGGCGCGCGCTCGATTGCCCGGACCGAACTGTACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCAGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTGGCTGCTGCACGACAGCg      <  1:330379/148‑1 (MQ=255)
                                                                                                                                                 aTGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTGGCTGCTGCACGACAGCGGc    <  7:149788/149‑1 (MQ=255)
                                                                                                                                                   gCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTGGCTGCTGCACGACAGCGGCCt  >  2:15022/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTTCCGCCCGGGTGAGTATGTGTATGCCGACAACAATGGCGTGCTGGTCTCGCCGAAACCGCTGAAAA‑TGCCGGAGTAATGCGCCGCACGCGCTGATGGAGCTTTGATGTTCGAGGAAGACAACGCGCAGTGGGGGCTGGTGCATGCCCTGGTGCTCGATGGCAAAGGTGGCGCGCGCTCGATTGCCCGTACCGAACTGGACGACCTGCAGTTGCAACCGGAGCAGAGCCTGTGGCTGCACTGGGACCGCAGCCATCCGCAAACCCGTTCCTGGCTGCTGCACGACAGCGGCCT  >  NC_002947/2377256‑2377550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑