| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,381,402 | T→G | 10.2% | intergenic (+31/+56) | oprF → / ← cobA | porin F/uroporphyrinogen‑III C‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,381,402 | 0 | T | G | 10.2% | 156.9 / 9.9 | 59 | intergenic (+31/+56) | oprF/cobA | porin F/uroporphyrinogen‑III C‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (21/32); new base G (4/2); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
CAGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGCCC > NC_002947/2381254‑2381549 | cAGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCt < 7:74622/149‑1 (MQ=255) aGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTc < 3:115746/149‑1 (MQ=255) ttGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCgg < 2:117600/148‑1 (MQ=255) ttGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCgg < 2:101363/148‑1 (MQ=255) aCTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGttt < 5:100761/149‑1 (MQ=255) cTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGtttt < 4:335147/149‑1 (MQ=255) ggTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGttttt < 3:166262/147‑1 (MQ=255) gCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGACGTGCAGTCAACCGTCGCGTTGAAGCTCACGTAGAAGCCCAGGCCAAGTAATAGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTATTCTTTGcc > 4:284912/1‑149 (MQ=255) tACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTg > 7:212100/1‑149 (MQ=255) aCGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg > 1:30491/1‑149 (MQ=255) cGGCGAAACCCGTCCGGTTTCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg < 3:225939/148‑1 (MQ=255) cGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg < 5:191066/148‑1 (MQ=255) ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGt < 2:109926/149‑1 (MQ=255) ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGt < 8:340505/133‑1 (MQ=255) ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGt > 7:340505/1‑133 (MQ=255) cGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGttt > 3:199291/1‑149 (MQ=255) aaCCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTGGGCCGGGTTTTTCTTTGCCTGGGTTTTa < 1:221429/148‑1 (MQ=255) ccGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCg > 3:20190/1‑149 (MQ=255) ttGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGaa < 4:199291/149‑1 (MQ=255) aaCGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTc > 6:302755/1‑149 (MQ=255) gCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCt > 2:249137/1‑148 (MQ=255) cACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCg < 6:19440/149‑1 (MQ=255) cACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCg < 8:50725/149‑1 (MQ=255) aCCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCt < 4:347676/109‑1 (MQ=255) aCCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCt > 3:347676/1‑109 (MQ=255) gaataaGGCCGTGCTGTCAACCTTCGCGTTGAATCTCATGTAGAACCCCAGGCCAATTAATTGGTTTTAAGCTTCATGAAAAACCCGGCCTCTGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCa < 7:135438/149‑1 (MQ=255) ccGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCtgcga < 5:302755/149‑1 (MQ=255) gTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTTGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCtgcgatt < 6:371164/149‑1 (MQ=255) gCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAg < 3:145500/138‑1 (MQ=255) gCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAg > 4:145500/1‑138 (MQ=255) gTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTg < 6:128925/94‑1 (MQ=255) gTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTg > 5:128925/1‑94 (MQ=255) cGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCAGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGccc < 2:30491/149‑1 (MQ=255) tCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCa < 8:212100/149‑1 (MQ=255) gTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGc > 2:49079/1‑149 (MQ=255) ttGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc > 2:83599/1‑149 (MQ=255) aaGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCctg < 4:20190/148‑1 (MQ=255) gCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCctgctg > 6:72041/1‑149 (MQ=255) aGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTcc > 6:296955/1‑149 (MQ=255) aGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCAGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTcc < 1:254124/149‑1 (MQ=255) gTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc < 2:321818/138‑1 (MQ=255) gTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc > 1:321818/1‑138 (MQ=255) aaGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAac > 3:268803/1‑148 (MQ=255) ccAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCCGCCTCGGCCGGGTTTTTCTTTGCCTGCGTTTTAACCGGCCCAGAAGGCCGCTCCTGCGGTCAGCCTGCGATAGCGATCCGCCCCATAGCCTGCTGCACCCCAACCACCt > 1:143825/1‑149 (MQ=255) aGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTcc < 5:72041/149‑1 (MQ=255) cAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCAGCTCCTGAGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCa > 3:367012/1‑148 (MQ=255) aTTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCa < 1:83599/149‑1 (MQ=255) aaGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAaccac > 4:192625/1‑126 (MQ=255) aaGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAaccac < 3:192625/126‑1 (MQ=255) aGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCgg < 4:268803/149‑1 (MQ=255) cATGAAAAACCCGGCCTGGGCCGGGTTTTTGTTTGCCTGGGTTTTAACCGTCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCGATAGGCTGCTGCTCCCCAACCTCCTCCCCAATCACCAGGATTGCCGGGCTa < 3:284912/148‑1 (MQ=255) aaagAAAAACCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGGTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTAc > 5:107526/4‑149 (MQ=255) gAAAAACCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGc > 1:158431/1‑148 (MQ=255) aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaa < 3:139282/149‑1 (MQ=255) aaaaCCCGGCGTCGGGCGTGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaa < 7:41630/148‑1 (MQ=255) aaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaac > 5:157317/1‑149 (MQ=255) aaaCCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTccc > 2:72407/1‑107 (MQ=255) aaaCCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTccc < 1:72407/107‑1 (MQ=255) cGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaaccaaac > 1:298539/1‑149 (MQ=255) cTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATACCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGccc < 2:116366/149‑1 (MQ=255) | CAGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGCCC > NC_002947/2381254‑2381549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |