Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,381,403 C→A 10.4% intergenic (+32/+55) oprF → / ← cobA porin F/uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,381,4030CA10.4% 154.6 / 6.3 58intergenic (+32/+55)oprF/cobAporin F/uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/28);  new base A (4/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGCCC  >  NC_002947/2381255‑2381549
                                                                                                                                                    |                                                                                                                                                  
aGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTc                                                                                                                                                    <  3:115746/149‑1 (MQ=255)
       ttGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCgg                                                                                                                                              <  2:101363/148‑1 (MQ=255)
       ttGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCgg                                                                                                                                              <  2:117600/148‑1 (MQ=255)
          aCTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGttt                                                                                                                                          <  5:100761/149‑1 (MQ=255)
           cTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGtttt                                                                                                                                         <  4:335147/149‑1 (MQ=255)
              ggTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGttttt                                                                                                                                        <  3:166262/147‑1 (MQ=255)
                   gCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGACGTGCAGTCAACCGTCGCGTTGAAGCTCACGTAGAAGCCCAGGCCAAGTAATAGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTATTCTTTGcc                                                                                                                                 >  4:284912/1‑149 (MQ=255)
                     tACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTg                                                                                                                               >  7:212100/1‑149 (MQ=255)
                      aCGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg                                                                                                                              >  1:30491/1‑149 (MQ=255)
                       cGGCGAAACCCGTCCGGTTTCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg                                                                                                                              <  3:225939/148‑1 (MQ=255)
                       cGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg                                                                                                                              <  5:191066/148‑1 (MQ=255)
                        ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGt                                                                                                                            <  2:109926/149‑1 (MQ=255)
                        ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGt                                                                                                                                            <  8:340505/133‑1 (MQ=255)
                        ggCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGt                                                                                                                                            >  7:340505/1‑133 (MQ=255)
                          cGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGttt                                                                                                                          >  3:199291/1‑149 (MQ=255)
                             aaCCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTGGGCCGGGTTTTTCTTTGCCTGGGTTTTa                                                                                                                        <  1:221429/148‑1 (MQ=255)
                                ccGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCg                                                                                                                    >  3:20190/1‑149 (MQ=255)
                                        ttGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGaa                                                                                                            <  4:199291/149‑1 (MQ=255)
                                                aaCGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTc                                                                                                    >  6:302755/1‑149 (MQ=255)
                                                   gCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCt                                                                                                  >  2:249137/1‑148 (MQ=255)
                                                     cACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCg                                                                                               <  6:19440/149‑1 (MQ=255)
                                                     cACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCg                                                                                               <  8:50725/149‑1 (MQ=255)
                                                      aCCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCt                                                                                                                                      <  4:347676/109‑1 (MQ=255)
                                                      aCCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCt                                                                                                                                      >  3:347676/1‑109 (MQ=255)
                                                         gaataaGGCCGTGCTGTCAACCTTCGCGTTGAATCTCATGTAGAACCCCAGGCCAATTAATTGGTTTTAAGCTTCATGAAAAACCCGGCCTCTGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCa                                                                                           <  7:135438/149‑1 (MQ=255)
                                                                 ccGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCtgcga                                                                                   <  5:302755/149‑1 (MQ=255)
                                                                   gTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTTGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCtgcgatt                                                                                 <  6:371164/149‑1 (MQ=255)
                                                                     gCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAg                                                                                          <  3:145500/138‑1 (MQ=255)
                                                                     gCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAg                                                                                          >  4:145500/1‑138 (MQ=255)
                                                                        gTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTg                                                                                                                                   >  5:128925/1‑94 (MQ=255)
                                                                        gTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTg                                                                                                                                   <  6:128925/94‑1 (MQ=255)
                                                                              cGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCAGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGccc                                                                      <  2:30491/149‑1 (MQ=255)
                                                                                tCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCa                                                                    <  8:212100/149‑1 (MQ=255)
                                                                                    gTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGc                                                                >  2:49079/1‑149 (MQ=255)
                                                                                     ttGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc                                                               >  2:83599/1‑149 (MQ=255)
                                                                                        aaGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCctg                                                             <  4:20190/148‑1 (MQ=255)
                                                                                          gCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCctgctg                                                          >  6:72041/1‑149 (MQ=255)
                                                                                              aGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTcc                                                      >  6:296955/1‑149 (MQ=255)
                                                                                              aGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCAGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTcc                                                      <  1:254124/149‑1 (MQ=255)
                                                                                                gTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc                                                               <  2:321818/138‑1 (MQ=255)
                                                                                                gTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGcc                                                               >  1:321818/1‑138 (MQ=255)
                                                                                                    aaGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAac                                                 >  3:268803/1‑148 (MQ=255)
                                                                                                        ccAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCCGCCTCGGCCGGGTTTTTCTTTGCCTGCGTTTTAACCGGCCCAGAAGGCCGCTCCTGCGGTCAGCCTGCGATAGCGATCCGCCCCATAGCCTGCTGCACCCCAACCACCt                                            >  1:143825/1‑149 (MQ=255)
                                                                                                          aGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTcc                                          <  5:72041/149‑1 (MQ=255)
                                                                                                              cAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCAGCTCCTGAGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCa                                       >  3:367012/1‑148 (MQ=255)
                                                                                                                    aTTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCa                                <  1:83599/149‑1 (MQ=255)
                                                                                                                             aaGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAaccac                                              >  4:192625/1‑126 (MQ=255)
                                                                                                                             aaGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAaccac                                              <  3:192625/126‑1 (MQ=255)
                                                                                                                              aGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCgg                      <  4:268803/149‑1 (MQ=255)
                                                                                                                                   cATGAAAAACCCGGCCTGGGCCGGGTTTTTGTTTGCCTGGGTTTTAACCGTCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCGATAGGCTGCTGCTCCCCAACCTCCTCCCCAATCACCAGGATTGCCGGGCTa                  <  3:284912/148‑1 (MQ=255)
                                                                                                                                   aaagAAAAACCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGGTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTAc                 >  5:107526/4‑149 (MQ=255)
                                                                                                                                      gAAAAACCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGc               >  1:158431/1‑148 (MQ=255)
                                                                                                                                       aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaa             <  3:139282/149‑1 (MQ=255)
                                                                                                                                        aaaaCCCGGCGTCGGGCGTGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaa             <  7:41630/148‑1 (MQ=255)
                                                                                                                                         aaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaac           >  5:157317/1‑149 (MQ=255)
                                                                                                                                         aaaCCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTccc                                                     <  1:72407/107‑1 (MQ=255)
                                                                                                                                         aaaCCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTccc                                                     >  2:72407/1‑107 (MQ=255)
                                                                                                                                              cGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaaccaaac      >  1:298539/1‑149 (MQ=255)
                                                                                                                                                  cTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATACCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGccc  <  2:116366/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCCGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCAAACGCCC  >  NC_002947/2381255‑2381549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑