Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,395,583 G→C 7.2% intergenic (‑191/+52) dacB ← / ← PP_2099 D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,395,5830GC 7.2% 213.7 / 3.4 67intergenic (‑191/+52)dacB/PP_2099D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base C (2/3);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

CGAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAG  >  NC_002947/2395437‑2395728
                                                                                                                                                    |                                                                                                                                                 
cGAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGaa                                                                                                                                                 >  2:230771/1‑149 (MQ=255)
 gAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAAt                                                                                                                                                >  5:224894/1‑149 (MQ=255)
        tACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTgg                                                                                                                                         >  7:127288/1‑149 (MQ=255)
          cccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGcc                                                                                                                                       <  4:349864/149‑1 (MQ=255)
           ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                      >  1:193791/1‑149 (MQ=255)
           ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                      >  5:349776/1‑149 (MQ=255)
            cTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCtt                                                                                                                                     >  4:170703/1‑149 (MQ=255)
            cTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtt                                                                                                                                     <  1:362895/149‑1 (MQ=255)
               cggcgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTc                                                                                                                                  <  3:132111/149‑1 (MQ=255)
                 gcgATGGAAAAGGGCTTTGGTGCTGTAAGAGATGCTGGCCGCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAg                                                                                                                                <  2:161955/149‑1 (MQ=255)
                  cgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAg                                                                                                                                >  4:147981/1‑148 (MQ=255)
                  cgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttttatt                                                                                                                               <  1:130147/149‑5 (MQ=255)
                         aaaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACGTAAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCaa                                                                                                                         <  1:106976/149‑1 (MQ=255)
                              gCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCttt                                                                                                                    >  6:342599/1‑148 (MQ=255)
                               cTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCa                                                                                                                  <  2:373034/149‑1 (MQ=255)
                                    gggCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCg                                                                                                             <  6:224894/149‑1 (MQ=255)
                                        tGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCTAAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAg                                                                                                          >  1:84829/1‑149 (MQ=255)
                                           aaGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGa                                                                                                      >  5:273998/1‑149 (MQ=255)
                                            agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                        >  2:47693/1‑146 (MQ=255)
                                            agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                        <  1:47693/146‑1 (MQ=255)
                                            agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGAc                                                                                                     <  3:320907/149‑1 (MQ=255)
                                                  gatgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                               >  8:48771/1‑149 (MQ=255)
                                                   atgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                               >  1:257059/1‑148 (MQ=255)
                                                    tgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCa                                                                                             <  8:127288/149‑1 (MQ=255)
                                                       ccccACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTc                                                                                          <  6:349776/149‑1 (MQ=255)
                                                         ccACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAg                                                                                        >  3:65931/1‑149 (MQ=255)
                                                          cACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGc                                                                                       >  5:208795/1‑149 (MQ=255)
                                                          cACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGc                                                                                       >  5:155286/1‑149 (MQ=255)
                                                           acgacgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGGCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGcc                                                                                      >  3:253053/1‑149 (MQ=255)
                                                               cgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                               >  1:329992/1‑136 (MQ=255)
                                                               cgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                               <  2:329992/136‑1 (MQ=255)
                                                                gTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                                 <  2:84829/149‑1 (MQ=255)
                                                                   aaCAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGa                                                                               >  2:338743/1‑148 (MQ=255)
                                                                   aaCAGCACGCCTTATGCCCC‑ACGCGCAGCCCCGATGCGTCACGTTCGACG‑GAAACGCATCGCCAATAAAAAGGCCACCCCAATGGGGGGGCCTTTTCAGCAATCCCCCTTTCAGCCCGAAGCTGACAAGTATCACTCACCCTTGTTGAt                                                                              <  6:368085/149‑1 (MQ=255)
                                                                     cAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAg                                                                            <  6:208795/149‑1 (MQ=255)
                                                                          cGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCttt                                                                       >  3:246682/1‑149 (MQ=255)
                                                                           gCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCtttt                                                                      >  6:2887/1‑149 (MQ=255)
                                                                                aTGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCgg                                                                  <  4:246682/148‑1 (MQ=255)
                                                                                  gCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTa                                                               >  7:180592/1‑149 (MQ=255)
                                                                                          gcgcAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCa                                                        <  2:193791/149‑1 (MQ=255)
                                                                                                   cgATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCagcagc                                               <  3:170703/149‑1 (MQ=255)
                                                                                                        cGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                   >  4:199212/1‑140 (MQ=255)
                                                                                                        cGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                   <  3:199212/140‑1 (MQ=255)
                                                                                                          tCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGa                                        >  6:373892/1‑149 (MQ=255)
                                                                                                             cGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACtt                                     <  3:147981/149‑1 (MQ=255)
                                                                                                              gTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                    >  8:274396/1‑149 (MQ=255)
                                                                                                                  gACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATc                                <  5:2887/149‑1 (MQ=255)
                                                                                                                   aCG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                       >  6:298721/1‑125 (MQ=255)
                                                                                                                   aCG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                       <  5:298721/125‑1 (MQ=255)
                                                                                                                     g‑gAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                             <  1:338743/149‑1 (MQ=255)
                                                                                                                        aaaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                             <  3:227305/147‑1 (MQ=255)
                                                                                                                        aaaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                             >  4:227305/1‑147 (MQ=255)
                                                                                                                            gCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                             <  2:45662/143‑1 (MQ=255)
                                                                                                                            gCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                             >  1:45662/1‑143 (MQ=255)
                                                                                                                             cATCGCCAATAAAAAGGCCACCCGCATGGGGGGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCCCTCAGCCTTGTTTATAGGCTTTTCCGGGTACCAGGCATCCAGGAGCGGGCTGACTTCGATCt                               <  7:330932/140‑1 (MQ=255)
                                                                                                                             cATCGCCAATAAAAAGGCAACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCCGCCCGAAGCTGACAAGTATCACAAAGCCTTCGTGCTAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCttagatcgg                       >  8:330932/1‑141 (MQ=255)
                                                                                                                                 gCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCt                  >  8:126894/1‑149 (MQ=255)
                                                                                                                                  ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTg                 >  3:93841/1‑149 (MQ=255)
                                                                                                                                  ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTg                 <  6:273998/149‑1 (MQ=255)
                                                                                                                                      tgaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGc             >  8:333071/3‑149 (MQ=255)
                                                                                                                                       gaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGGCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGcc            >  1:285774/2‑149 (MQ=255)
                                                                                                                                         aaaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                    <  7:356170/123‑1 (MQ=255)
                                                                                                                                         aaaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                    >  8:356170/1‑123 (MQ=255)
                                                                                                                                          aaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCttt         <  2:319397/149‑1 (MQ=255)
                                                                                                                                           aGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTg        <  7:48771/149‑1 (MQ=255)
                                                                                                                                              ccACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGc     <  8:180592/149‑1 (MQ=255)
                                                                                                                                               cACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGc     >  4:76239/1‑148 (MQ=255)
                                                                                                                                                    gAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAg  <  4:122589/146‑1 (MQ=255)
                                                                                                                                                    gAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAg  >  3:122589/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAG  >  NC_002947/2395437‑2395728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑