Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,395,587 G→C 5.9% intergenic (‑195/+48) dacB ← / ← PP_2099 D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,395,5870GC 5.9% 208.8 / 2.6 68intergenic (‑195/+48)dacB/PP_2099D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

TACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAGGCTTC  >  NC_002947/2395445‑2395733
                                                                                                                                                |                                                                                                                                                  
tACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTgg                                                                                                                                              >  7:127288/1‑149 (MQ=255)
  cccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGcc                                                                                                                                            <  4:349864/149‑1 (MQ=255)
   ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                           >  1:193791/1‑149 (MQ=255)
   ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                           >  5:349776/1‑149 (MQ=255)
    cTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCtt                                                                                                                                          >  4:170703/1‑149 (MQ=255)
    cTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtt                                                                                                                                          <  1:362895/149‑1 (MQ=255)
       cggcgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTc                                                                                                                                       <  3:132111/149‑1 (MQ=255)
         gcgATGGAAAAGGGCTTTGGTGCTGTAAGAGATGCTGGCCGCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAg                                                                                                                                     <  2:161955/149‑1 (MQ=255)
          cgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAg                                                                                                                                     >  4:147981/1‑148 (MQ=255)
          cgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttttatt                                                                                                                                    <  1:130147/149‑5 (MQ=255)
                 aaaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACGTAAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCaa                                                                                                                              <  1:106976/149‑1 (MQ=255)
                      gCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCttt                                                                                                                         >  6:342599/1‑148 (MQ=255)
                       cTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCa                                                                                                                       <  2:373034/149‑1 (MQ=255)
                            gggCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCg                                                                                                                  <  6:224894/149‑1 (MQ=255)
                                tGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCTAAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAg                                                                                                               >  1:84829/1‑149 (MQ=255)
                                   aaGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGa                                                                                                           >  5:273998/1‑149 (MQ=255)
                                    agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                             <  1:47693/146‑1 (MQ=255)
                                    agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                             >  2:47693/1‑146 (MQ=255)
                                    agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGAc                                                                                                          <  3:320907/149‑1 (MQ=255)
                                          gatgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                                    >  8:48771/1‑149 (MQ=255)
                                           atgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                                    >  1:257059/1‑148 (MQ=255)
                                            tgGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCa                                                                                                  <  8:127288/149‑1 (MQ=255)
                                               ccccACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTc                                                                                               <  6:349776/149‑1 (MQ=255)
                                                 ccACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAg                                                                                             >  3:65931/1‑149 (MQ=255)
                                                  cACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGc                                                                                            >  5:208795/1‑149 (MQ=255)
                                                  cACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGc                                                                                            >  5:155286/1‑149 (MQ=255)
                                                   acgacgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGGCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGcc                                                                                           >  3:253053/1‑149 (MQ=255)
                                                       cgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                                    <  2:329992/136‑1 (MQ=255)
                                                       cgTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                                    >  1:329992/1‑136 (MQ=255)
                                                        gTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                                      <  2:84829/149‑1 (MQ=255)
                                                           aaCAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGa                                                                                    >  2:338743/1‑148 (MQ=255)
                                                           aaCAGCACGCCTTATGCCCC‑ACGCGCAGCCCCGATGCGTCACGTTCGACG‑GAAACGCATCGCCAATAAAAAGGCCACCCCAATGGGGGGGCCTTTTCAGCAATCCCCCTTTCAGCCCGAAGCTGACAAGTATCACTCACCCTTGTTGAt                                                                                   <  6:368085/149‑1 (MQ=255)
                                                             cAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAg                                                                                 <  6:208795/149‑1 (MQ=255)
                                                                  cGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCttt                                                                            >  3:246682/1‑149 (MQ=255)
                                                                   gCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCtttt                                                                           >  6:2887/1‑149 (MQ=255)
                                                                        aTGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCgg                                                                       <  4:246682/148‑1 (MQ=255)
                                                                          gCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTa                                                                    >  7:180592/1‑149 (MQ=255)
                                                                                  gcgcAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCa                                                             <  2:193791/149‑1 (MQ=255)
                                                                                           cgATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCagcagc                                                    <  3:170703/149‑1 (MQ=255)
                                                                                                cGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                        >  4:199212/1‑140 (MQ=255)
                                                                                                cGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                        <  3:199212/140‑1 (MQ=255)
                                                                                                  tCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGa                                             >  6:373892/1‑149 (MQ=255)
                                                                                                     cGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACtt                                          <  3:147981/149‑1 (MQ=255)
                                                                                                      gTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                         >  8:274396/1‑149 (MQ=255)
                                                                                                          gACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATc                                     <  5:2887/149‑1 (MQ=255)
                                                                                                           aCG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                            >  6:298721/1‑125 (MQ=255)
                                                                                                           aCG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                            <  5:298721/125‑1 (MQ=255)
                                                                                                             g‑gAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                                  <  1:338743/149‑1 (MQ=255)
                                                                                                                aaaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                                  <  3:227305/147‑1 (MQ=255)
                                                                                                                aaaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                                  >  4:227305/1‑147 (MQ=255)
                                                                                                                    gCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                                  >  1:45662/1‑143 (MQ=255)
                                                                                                                    gCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTg                                  <  2:45662/143‑1 (MQ=255)
                                                                                                                     cATCGCCAATAAAAAGGCCACCCGCATGGGGGGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCCCTCAGCCTTGTTTATAGGCTTTTCCGGGTACCAGGCATCCAGGAGCGGGCTGACTTCGATCt                                    <  7:330932/140‑1 (MQ=255)
                                                                                                                     cATCGCCAATAAAAAGGCAACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCCGCCCGAAGCTGACAAGTATCACAAAGCCTTCGTGCTAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCttagatcgg                            >  8:330932/1‑141 (MQ=255)
                                                                                                                         gCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCt                       >  8:126894/1‑149 (MQ=255)
                                                                                                                          ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTg                      >  3:93841/1‑149 (MQ=255)
                                                                                                                          ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTg                      <  6:273998/149‑1 (MQ=255)
                                                                                                                              tgaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGc                  >  8:333071/3‑149 (MQ=255)
                                                                                                                               gaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGGCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGcc                 >  1:285774/2‑149 (MQ=255)
                                                                                                                                 aaaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                         <  7:356170/123‑1 (MQ=255)
                                                                                                                                 aaaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                         >  8:356170/1‑123 (MQ=255)
                                                                                                                                  aaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCttt              <  2:319397/149‑1 (MQ=255)
                                                                                                                                   aGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTg             <  7:48771/149‑1 (MQ=255)
                                                                                                                                      ccACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGc          <  8:180592/149‑1 (MQ=255)
                                                                                                                                       cACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGc          >  4:76239/1‑148 (MQ=255)
                                                                                                                                            gAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAg       <  4:122589/146‑1 (MQ=255)
                                                                                                                                            gAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAg       >  3:122589/1‑146 (MQ=255)
                                                                                                                                              aTGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAGGCTTc  <  3:76239/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCC‑AAGCGCAGCCGCGATGCGTCACGTTCGACG‑GAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAGGCTTC  >  NC_002947/2395445‑2395733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑