| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,474,154 | A→G | 14.7% | intergenic (‑59/+121) | PP_2167 ← / ← tal | PhoD‑like phosphatase/transaldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,474,154 | 0 | A | G | 14.7% | 72.6 / 6.1 | 34 | intergenic (‑59/+121) | PP_2167/tal | PhoD‑like phosphatase/transaldolase |
| Reads supporting (aligned to +/- strand): ref base A (13/16); new base G (3/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01 | |||||||||||
GTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCA > NC_002947/2474007‑2474302 | gTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCCCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCa < 2:288147/148‑1 (MQ=255) gAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAg < 7:43744/149‑1 (MQ=255) cagacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGc < 1:294043/149‑1 (MQ=255) agacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGA‑TCGATGTAAGGGCg < 4:378701/149‑1 (MQ=37) gacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCgg < 3:126041/149‑1 (MQ=255) cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCATCGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGA‑TCGATGTAAgggcgggc < 1:83511/149‑1 (MQ=37) ggCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg < 1:345480/149‑1 (MQ=255) ggCGAGAACGAGGGGCAACGGGTCAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCTA‑TCAGTGTAGCAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg < 4:362800/149‑1 (MQ=255) gggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTTCCTCGA‑TGAGTGTAGGCGCGGGCTTGGCCGCGGACAGGCCCGCGAATTGAATACACAACTAAGGCGGGGgaaac > 1:109178/1‑148 (MQ=255) aCGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACg < 3:188199/149‑1 (MQ=255) gggTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACggg < 1:20165/149‑1 (MQ=255) ggTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACt < 1:309360/149‑1 (MQ=255) gCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTccc < 6:323823/149‑1 (MQ=255) cTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATAATGAACACTTcccg > 5:193981/1‑149 (MQ=255) tGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTc < 7:103302/149‑1 (MQ=255) ggcggcCGGTTCGCGGGCACGCCCCCCATAACATCTA‑ACAGTGTAGCCCCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCaaa < 1:180739/149‑1 (MQ=255) cggcCTGTTCGCGGGCAAGCCCGCCCGTACATCGC‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACCGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAACCAAAACGGGCGGGAAAGACTGACCACTTCCCGCCCGGCAGGCAAaca > 5:384290/1‑149 (MQ=38) gcCTGTTCGCGGGCAAGCCCGCCGTTACAGAGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAAGACTTCCAGCCCGTCAGGCAAaaac > 6:317729/1‑148 (MQ=38) cTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa < 6:5215/149‑1 (MQ=255) cTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGGGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGa > 5:5215/1‑148 (MQ=255) tGTTCGCGGGCAAGCCCGCCCTTACATCGAGTCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa > 1:233111/1‑149 (MQ=255) ttCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGTAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt < 7:218647/148‑1 (MQ=255) tCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCATACACGAACTGt > 4:253487/1‑149 (MQ=255) gggCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACATACCCGAGAATTCAATACACAACTAAGGCGGGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTAt > 1:296640/1‑147 (MQ=255) aaGCCCGCCCTTACATCGA‑TCAGTGTAGGCGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAACCGGGCGGAAAATACTGCACACTTCCCGCCCGTCTGGCACACCCGAACTGTATCAGGcc > 4:4497/1‑149 (MQ=255) gcccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt > 2:209458/1‑149 (MQ=37) ccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt > 1:246719/1‑149 (MQ=37) ccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCttt > 3:213482/1‑148 (MQ=37) ttACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa > 7:352824/1‑149 (MQ=255) aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt < 1:298703/139‑1 (MQ=255) aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc < 7:347070/147‑1 (MQ=255) aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc > 8:347070/1‑147 (MQ=255) aTCGA‑TCAGGGGAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAACCAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCGAACACGAACTGTATCAGGCCtgtg > 2:298703/1‑136 (MQ=255) tCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc > 8:310638/1‑149 (MQ=255) atcgtccAGGGTCGGAGCGGCCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTACGGCGTGGCAAACACACCGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCC‑TTTCAGCTATCAAc < 6:384290/143‑1 (MQ=255) aGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCa < 5:390731/149‑1 (MQ=255) | GTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCA > NC_002947/2474007‑2474302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |