Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,474,155 G→A 14.3% intergenic (‑60/+120) PP_2167 ← / ← tal PhoD‑like phosphatase/transaldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,474,1550GA14.3% 82.8 / 5.6 35intergenic (‑60/+120)PP_2167/talPhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base G (13/17);  new base A (3/2);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01

GAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAA  >  NC_002947/2474012‑2474303
                                                                                                                                                |                                                                                                                                                    
gAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAg                                                                                                                                                 <  7:43744/149‑1 (MQ=255)
    cagacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGc                                                                                                                                             <  1:294043/149‑1 (MQ=255)
     agacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGA‑TCGATGTAAGGGCg                                                                                                                                            <  4:378701/149‑1 (MQ=37)
      gacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCgg                                                                                                                                           <  3:126041/149‑1 (MQ=255)
        cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCATCGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGA‑TCGATGTAAgggcgggc                                                                                                                                         <  1:83511/149‑1 (MQ=37)
                                                ggCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                                 <  1:345480/149‑1 (MQ=255)
                                                ggCGAGAACGAGGGGCAACGGGTCAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCTA‑TCAGTGTAGCAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                                 <  4:362800/149‑1 (MQ=255)
                                                            gggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTTCCTCGA‑TGAGTGTAGGCGCGGGCTTGGCCGCGGACAGGCCCGCGAATTGAATACACAACTAAGGCGGGGgaaac                                                                                      >  1:109178/1‑148 (MQ=255)
                                                                 aCGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACg                                                                                <  3:188199/149‑1 (MQ=255)
                                                                   gggTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACggg                                                                              <  1:20165/149‑1 (MQ=255)
                                                                              ggTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACt                                                                   <  1:309360/149‑1 (MQ=255)
                                                                                         gCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTccc                                                        <  6:323823/149‑1 (MQ=255)
                                                                                          cTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATAATGAACACTTcccg                                                       >  5:193981/1‑149 (MQ=255)
                                                                                                tGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTc                                                 <  7:103302/149‑1 (MQ=255)
                                                                                                       ggcggcCGGTTCGCGGGCACGCCCCCCATAACATCTA‑ACAGTGTAGCCCCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCaaa                                          <  1:180739/149‑1 (MQ=255)
                                                                                                         cggcCTGTTCGCGGGCAAGCCCGCCCGTACATCGC‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACCGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAACCAAAACGGGCGGGAAAGACTGACCACTTCCCGCCCGGCAGGCAAaca                                        >  5:384290/1‑149 (MQ=38)
                                                                                                           gcCTGTTCGCGGGCAAGCCCGCCGTTACAGAGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAAGACTTCCAGCCCGTCAGGCAAaaac                                       >  6:317729/1‑148 (MQ=38)
                                                                                                             cTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa                                    <  6:5215/149‑1 (MQ=255)
                                                                                                             cTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGGGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGa                                     >  5:5215/1‑148 (MQ=255)
                                                                                                              tGTTCGCGGGCAAGCCCGCCCTTACATCGAGTCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa                                    >  1:233111/1‑149 (MQ=255)
                                                                                                                ttCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGTAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt                                  <  7:218647/148‑1 (MQ=255)
                                                                                                                 tCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCATACACGAACTGt                                >  4:253487/1‑149 (MQ=255)
                                                                                                                     gggCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACATACCCGAGAATTCAATACACAACTAAGGCGGGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTAt                              >  1:296640/1‑147 (MQ=255)
                                                                                                                         aaGCCCGCCCTTACATCGA‑TCAGTGTAGGCGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAACCGGGCGGAAAATACTGCACACTTCCCGCCCGTCTGGCACACCCGAACTGTATCAGGcc                        >  4:4497/1‑149 (MQ=255)
                                                                                                                           gcccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt                      >  2:209458/1‑149 (MQ=37)
                                                                                                                             ccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt                    >  1:246719/1‑149 (MQ=37)
                                                                                                                             ccgctcCTACACTGA‑TCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCttt                     >  3:213482/1‑148 (MQ=37)
                                                                                                                                   ttACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa              >  7:352824/1‑149 (MQ=255)
                                                                                                                                       aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt                    <  1:298703/139‑1 (MQ=255)
                                                                                                                                       aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc            >  8:347070/1‑147 (MQ=255)
                                                                                                                                       aTCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc            <  7:347070/147‑1 (MQ=255)
                                                                                                                                       aTCGA‑TCAGGGGAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAACCAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCGAACACGAACTGTATCAGGCCtgtg                    >  2:298703/1‑136 (MQ=255)
                                                                                                                                        tCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc         >  8:310638/1‑149 (MQ=255)
                                                                                                                                        atcgtccAGGGTCGGAGCGGCCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTACGGCGTGGCAAACACACCGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCC‑TTTCAGCTATCAAc         <  6:384290/143‑1 (MQ=255)
                                                                                                                                               aGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCa   <  5:390731/149‑1 (MQ=255)
                                                                                                                                                gtgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTtctc    >  5:173377/1‑147 (MQ=255)
                                                                                                                                                gtgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCaa  <  3:366062/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGA‑TCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAA  >  NC_002947/2474012‑2474303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑