Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,526,412	A→T	5.5%	K219M (AAG→ATG)	PP_2215 →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,526,412	0	A	T	5.5%	249.0 / 2.3	82	K219M (AAG→ATG)	PP_2215	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (38/39);  new base T (3/2);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGCGCCGCTCCGAG  >  NC_002947/2526265‑2526557                                                                                                                                                     |                                                                                                                                                        cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGCGCGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCaa                                                                                                                                                         <  1:290903/149‑1 (MQ=255) cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCaa                                                                                                                                                         <  1:338224/148‑1 (MQ=255) cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        >  2:227708/1‑149 (MQ=255) cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        >  8:195251/1‑149 (MQ=255) cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        <  2:281981/149‑1 (MQ=255) cccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        <  7:204254/149‑1 (MQ=255)  ccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAgg                                                                                                                                                       <  2:203481/149‑1 (MQ=255)  ccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAgg                                                                                                                                                       <  7:193970/149‑1 (MQ=255)  ccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        >  1:299674/1‑148 (MQ=255)  ccAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAg                                                                                                                                                        >  8:193970/1‑148 (MQ=255)   cAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGa                                                                                                                                                      >  2:112948/1‑149 (MQ=255)      gCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGGAAGGAAAAGCGCGTCATCAAGGATGa                                                                                                                                                   <  7:162801/149‑1 (MQ=255)         aaTGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGAc                                                                                                                                                  >  6:105256/1‑147 (MQ=255)          aTGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGag                                                                                                                                               >  2:15328/1‑149 (MQ=255)             ccTTCAGCCGCGAGGCCCAGGACCAGTTCGCCCTCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGag‑ca                                                                                                                                            <  8:166093/148‑1 (MQ=255)             ccTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGag‑ca                                                                                                                                            <  3:103061/148‑1 (MQ=255)               ttCAGCCGCGAGGCCCAGGACCGGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAgcc                                                                                                                                         >  2:205116/1‑149 (MQ=255)               ttCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAgcc                                                                                                                                         >  8:108086/1‑149 (MQ=255)                  aGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAgccgc‑c                                                                                                                                     <  5:242135/149‑1 (MQ=255)                    ccGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGc‑cc                                                                                                                                    >  8:7302/1‑148 (MQ=255)                      gcgAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑TCAAg                                                                                                                                 <  5:57105/149‑1 (MQ=255)                          ggCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGgcgc                                                                                                                             <  7:108086/149‑1 (MQ=255)                            cccAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGt                                                                                                                           >  1:356258/1‑149 (MQ=255)                            cccAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGt                                                                                                                           >  2:306148/1‑149 (MQ=255)                              cAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCt                                                                                                                         >  3:342027/1‑149 (MQ=255)                               aGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTg                                                                                                                        <  3:125806/149‑1 (MQ=255)                                ggACCACTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTg‑g                                                                                                                      <  8:235632/149‑1 (MQ=255)                                  aCCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGTGGGCAAGGAAAAGCGTGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GAc                                                                                                                    <  1:271174/149‑1 (MQ=255)                                    cAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GAc                                                                                                                    >  1:385608/1‑147 (MQ=255)                                    cAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GAc                                                                                                                    >  3:274331/1‑147 (MQ=255)                                    cAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACaa                                                                                                                  <  7:195251/149‑1 (MQ=255)                                    cAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACaa                                                                                                                  <  8:147487/149‑1 (MQ=255)                                    cAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGCCTG‑GACaa                                                                                                                  <  4:80211/149‑1 (MQ=255)                                      gTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGTGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGa                                                                                                                <  3:105278/149‑1 (MQ=255)                                           ccATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTg                                                                                                            >  7:246901/1‑147 (MQ=255)                                           ccATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTg                                                                                                            <  8:246901/147‑1 (MQ=255)                                              tCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAg                                                                                                       >  5:139025/1‑149 (MQ=255)                                                  cTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTc                                                                                                    >  7:18425/1‑148 (MQ=255)                                                           ccGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGt                                                                                          >  2:230252/1‑149 (MQ=255)                                                           ccGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGt                                                                                          >  5:110532/1‑149 (MQ=255)                                                           ccGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGt                                                                                          >  7:165270/1‑149 (MQ=255)                                                                cgcAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGt                                                                                     <  4:342027/149‑1 (MQ=255)                                                                   aGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAg                                                                                                       >  6:2536/1‑128 (MQ=255)                                                                   aGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAg                                                                                                       <  5:2536/128‑1 (MQ=255)                                                                   aGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTg                                                                                    >  3:40551/1‑147 (MQ=255)                                                                   aGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTg                                                                                    >  2:44514/1‑147 (MQ=255)                                                                     gAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAgg                                                                                >  5:182720/1‑149 (MQ=255)                                                                      aaGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAgc                                                                                <  1:227708/148‑2 (MQ=255)                                                                      aaGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGGCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAggc                                                                               <  2:299674/149‑1 (MQ=255)                                                                           aTCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGTGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCAc                                                                         >  2:125910/1‑149 (MQ=255)                                                                           aTCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCAc                                                                         >  2:3089/1‑149 (MQ=255)                                                                              agcagcGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGt                                                                      <  1:15328/149‑1 (MQ=255)                                                                                cagcGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑a                                                                   <  2:359795/149‑1 (MQ=255)                                                                                     gCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑Acggcg                                                              <  2:321673/149‑1 (MQ=255)                                                                                     gCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGCGAAGGCG‑GCACCGTG‑Acggcg                                                              <  1:112948/149‑1 (MQ=255)                                                                                             gccgc‑cGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGc                                                      >  6:96969/1‑149 (MQ=255)                                                                                                      gaTCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAGTGAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTc                                                >  1:111909/1‑148 (MQ=255)                                                                                                         cGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGAttt                                           >  3:348891/1‑149 (MQ=255)                                                                                                          gTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTc                                          <  6:182720/149‑1 (MQ=255)                                                                                                              cGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAc                                      >  3:198106/1‑149 (MQ=255)                                                                                                               ggTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcg                                     >  8:287640/1‑149 (MQ=255)                                                                                                                       tCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcg                             <  1:205116/149‑1 (MQ=255)                                                                                                                         aCCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcggc                           >  6:341490/1‑149 (MQ=255)                                                                                                                         aCCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcggc                           >  1:245978/1‑149 (MQ=255)                                                                                                                          ccGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcggcg                          <  1:230252/149‑1 (MQ=255)                                                                                                                              gggCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTGCACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcggcgctg                       <  1:80240/149‑1 (MQ=255)                                                                                                                              gggCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTgg                      <  4:40551/149‑1 (MQ=255)                                                                                                                               ggCAAGGAAACGCGCTTCATCATGGTTGTCGAG‑CTGCGGC‑CCACGGCGTGTCTGCTAGAAGACTTTAAAAATTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGAcggcgctgcggcgctg                       <  1:125910/149‑1 (MQ=255)                                                                                                                                gCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTa                    <  8:18425/149‑1 (MQ=255)                                                                                                                                 cAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTAc                   <  2:356258/149‑1 (MQ=255)                                                                                                                                 cAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTAc                   <  2:385608/149‑1 (MQ=255)                                                                                                                                 cAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTAc                   <  4:274331/149‑1 (MQ=255)                                                                                                                                     gAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGAt               <  7:7302/149‑1 (MQ=255)                                                                                                                                     gAAAAGCGCGTCACCAAGGAGCACGAG‑AAGCCGC‑CCACGCCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCGTTCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGa                <  1:306148/149‑1 (MQ=255)                                                                                                                                       aaaGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATgc             <  2:312760/149‑1 (MQ=255)                                                                                                                                          gcgcGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGcgcc          >  3:335354/1‑149 (MQ=255)                                                                                                                                           ctcgTCATCCTTGATGACGCG‑CAGCCGCGCCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATgc             >  1:221207/3‑146 (MQ=255)                                                                                                                                           ctcgTCATCCTTGATGACGCG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATgc             <  2:221207/143‑1 (MQ=255)                                                                                                                                           ctcgTCATCCTTGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGcgccg         >  7:256662/3‑149 (MQ=255)                                                                                                                                           ctcgTCATCCTTGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCAACGAG‑ACGGCGGCCAACGCCAGTGCGATTTCCGACGGCGCTGCGGCGCGGGTACTGATGagccg         >  7:251662/3‑149 (MQ=255)                                                                                                                                            gcgTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGATTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGATGCGGCGCTGGTACTGATGcgccgc        >  7:299087/1‑149 (MQ=255)                                                                                                                                                catcaAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGCGCCGCTcc     >  4:56946/1‑148 (MQ=255)                                                                                                                                                  tcaAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGCGCCGCTCCGAg  <  1:10790/149‑1 (MQ=255)                                                                                                                                                  tcaAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGCGCCGCTCCGAg  >  5:137134/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                        CCCAGGCCAATGCCTTCAGCCGCGAGGCCCAGGACCAGTTCGCCATCGCCTCGCTGACCCGAGCGCAGGAAGCCATCAGCAGCGGCCGTTTTGCCGC‑CGAGATCGTGCCGGTGGAAGTCACCGAGGGCAAGGAAAAGCGCGTCATCAAGGATGACGAG‑CAGCCGC‑CCAAGGCGCGTCTG‑GACAAGA‑TTGCGCAGCTCAAACCGGCGTTTCGTGAAGGCG‑GCACCGTG‑ACGGCGGCCAACGCCAGTTCGATTTCCGACGGCGCTGCGGCGCTGGTACTGATGCGCCGCTCCGAG  >  NC_002947/2526265‑2526557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑