Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,576,436	C→G	7.4%	A538G (GCC→GGC)	PP_2258 →	sensory box protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,576,436	0	C	G	7.4%	212.5 / 6.8	68	A538G (GCC→GGC)	PP_2258	sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (27/36);  new base G (3/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

GACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCG  >  NC_002947/2576288‑2576583                                                                                                                                                     |                                                                                                                                                    gACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGc                                                                                                                                                     >  7:350508/1‑149 (MQ=255)  aCCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGcc                                                                                                                                                    >  4:255660/1‑149 (MQ=255)   ccAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGcc                                                                                                                                                    <  2:258861/148‑1 (MQ=255)    cAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCg                                                                                                                                                  >  3:78020/1‑149 (MQ=255)        acgacCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCg                                                                                                                                              <  4:173230/149‑1 (MQ=255)          gacCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGAt                                                                                                                                            <  8:350508/149‑1 (MQ=255)              tGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGccc                                                                                                                                        >  4:90018/1‑149 (MQ=255)                gcgcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCCGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGc                                                                                                                                      <  7:30755/149‑1 (MQ=255)                 cgcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcagtaa                                                                                                                                     <  6:287951/149‑6 (MQ=255)                  gcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGc                                                                                                                                      >  6:165816/1‑147 (MQ=255)                  gcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                                                    <  3:255660/149‑1 (MQ=255)                   cTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAg                                                                                                                                   <  4:77166/149‑1 (MQ=255)                    tGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGt                                                                                                                                  >  1:28066/1‑149 (MQ=255)                              aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcag                                                                                                                                        >  5:24563/1‑131 (MQ=255)                              aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcag                                                                                                                                        <  6:24563/133‑3 (MQ=255)                                  aTCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTg                                                                                                                    <  5:151786/149‑1 (MQ=255)                                   tCGGCCTGGCGCGAGCGTTTGGCCGGGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTgg                                                                                                                   <  3:90018/149‑1 (MQ=255)                                      gCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGca                                                                                                                >  8:212233/1‑149 (MQ=255)                                          ggCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAgg                                                                                                            >  7:272305/1‑149 (MQ=255)                                          ggCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAgg                                                                                                            >  1:299521/1‑149 (MQ=255)                                           gcgcGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTTGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAg                                                                                                             <  3:67814/147‑1 (MQ=255)                                                aGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTAcc                                                                                                      <  1:241801/149‑1 (MQ=255)                                                         ccGCGAGGTGATTGCCGAGGGGATGGAGAGTGTCTAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTTCTGCATCGCCAGGCCGATGCCCGCCAGTGACGTGCACGCCTGGGCACAGGACGACCAGCAGCcag                                                                                             >  8:194576/1‑149 (MQ=255)                                                           gcgAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtg                                                                                           <  3:52975/149‑1 (MQ=255)                                                             gAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtgca                                                                                         <  4:78020/149‑1 (MQ=255)                                                               ggTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtgcagt                                                                                       >  3:17592/1‑149 (MQ=255)                                                                   aTTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGc                                                                                    <  6:384511/148‑1 (MQ=255)                                                                    ttGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGt                                                                                  <  8:217159/149‑1 (MQ=255)                                                                     tGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGt                                                                                  <  4:196770/148‑1 (MQ=255)                                                                        cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAcc                                                                              >  2:77320/1‑149 (MQ=255)                                                                        cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAcc                                                                              >  1:353675/1‑149 (MQ=255)                                                                                  gagaGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGtc                                                                    <  2:28066/149‑1 (MQ=255)                                                                                  gagaGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGtc                                                                    <  2:179806/149‑1 (MQ=255)                                                                                         tCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCg                                                             <  5:165816/149‑1 (MQ=255)                                                                                           gatcACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCTATGCGCGCCAGTGACGTGCTAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCggg                                                           <  7:250925/146‑1 (MQ=255)                                                                                            aGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGc                                                          >  5:364872/1‑149 (MQ=255)                                                                                              cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACcagcag                                                                                                 >  4:37355/1‑108 (MQ=255)                                                                                              cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACcagcag                                                                                                 <  3:37355/108‑1 (MQ=255)                                                                                              cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCg                                                        >  7:26232/1‑149 (MQ=255)                                                                                                      ggTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCtt                                                 >  8:365077/1‑148 (MQ=255)                                                                                                      ggTACGGATGGGCCTTGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTg                                                <  4:14882/149‑1 (MQ=255)                                                                                                       gTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                      >  4:188130/1‑142 (MQ=255)                                                                                                       gTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                      <  3:188130/142‑1 (MQ=255)                                                                                                        tACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGAc                                              >  4:173013/1‑149 (MQ=255)                                                                                                             aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                      <  5:78415/136‑1 (MQ=255)                                                                                                             aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                      >  6:78415/1‑136 (MQ=255)                                                                                                             aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCa                                         >  6:96725/1‑149 (MQ=255)                                                                                                                 gCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACggg                                     <  1:77320/149‑1 (MQ=255)                                                                                                                    tGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACgggggg                                  >  6:307867/1‑149 (MQ=255)                                                                                                                    tGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACgggggg                                  >  3:97502/1‑149 (MQ=255)                                                                                                                         tGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCa                             >  5:250930/1‑149 (MQ=255)                                                                                                                          gCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCAc                            <  7:251922/149‑1 (MQ=255)                                                                                                                          gCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCAc                            <  3:219203/149‑1 (MQ=255)                                                                                                                                 ggCGCAGGGTTACTGAATAGCCAGGACGATGCCCGCAAGTGACGAGCAATACTGGGCACAGGCCTACCAGAACACACTCAACTCCCGAGACACATGAAGGTCATCAGCGGGCCGTCGACTTGCCCTCAACGCCGGGCGCAACCTGCTTg                     >  6:248314/1‑149 (MQ=255)                                                                                                                                  gcgcAGGGTTACTGCTTCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGGCTGGGCAGAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGGGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCCTGt                    <  2:203408/149‑1 (MQ=255)                                                                                                                                  gcgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGt                    <  8:26232/149‑1 (MQ=255)                                                                                                                                  gcgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCGGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCtt                      <  7:6092/147‑1 (MQ=255)                                                                                                                                   cgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                   <  4:364929/149‑1 (MQ=255)                                                                                                                                    gcAGGGTTACTGCATCGCTCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                   >  8:233613/1‑148 (MQ=255)                                                                                                                                    gcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                   >  7:143281/1‑148 (MQ=255)                                                                                                                                      aGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGAt                <  4:12064/149‑1 (MQ=255)                                                                                                                                      aGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGAt                <  4:345830/149‑1 (MQ=255)                                                                                                                                       gggTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATc               <  4:97502/149‑1 (MQ=255)                                                                                                                                       gggTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATc               <  6:364872/149‑1 (MQ=255)                                                                                                                                         gTTACTGCATCGCCCGTCCGATGCACGCCAGTGACGTGCTAGCGTGGGCGCAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAGCCTTGAAGGTCATCAGCGGTGCGTGGTCTTGACCTGCGCTGGGGGCGCCACAGTCTAGTGGATCAg             >  4:82268/1‑149 (MQ=255)                                                                                                                                            aCTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAggg           <  7:365077/148‑1 (MQ=255)                                                                                                                                             gggCATCGGCCGGGCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGt         >  6:239155/3‑149 (MQ=255)                                                                                                                                             gggCATCGGCCGGGCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGt         >  3:372576/3‑149 (MQ=255)                                                                                                                                                cATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTg        <  8:143281/147‑1 (MQ=255)                                                                                                                                                 aTCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCt     >  8:83592/1‑149 (MQ=255)                                                                                                                                                   ctcccGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTc   <  7:194576/147‑1 (MQ=255)                                                                                                                                                    gCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCg  <  2:299521/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCG  >  NC_002947/2576288‑2576583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑