Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,576,441	C→G	7.3%	P540A (CCG→GCG)	PP_2258 →	sensory box protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,576,441	0	C	G	7.3%	217.9 / 6.7	69	P540A (CCG→GCG)	PP_2258	sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (26/37);  new base G (3/2);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01

ACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCGA  >  NC_002947/2576295‑2576584                                                                                                                                                   |                                                                                                                                                acgacCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCg                                                                                                                                               <  4:173230/149‑1 (MQ=255)   gacCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGAt                                                                                                                                             <  8:350508/149‑1 (MQ=255)       tGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGccc                                                                                                                                         >  4:90018/1‑149 (MQ=255)         gcgcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCCGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGc                                                                                                                                       <  7:30755/149‑1 (MQ=255)          cgcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcagtaa                                                                                                                                      <  6:287951/149‑6 (MQ=255)           gcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGc                                                                                                                                       >  6:165816/1‑147 (MQ=255)           gcTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                                                     <  3:255660/149‑1 (MQ=255)            cTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAg                                                                                                                                    <  4:77166/149‑1 (MQ=255)             tGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGt                                                                                                                                   >  1:28066/1‑149 (MQ=255)                       aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcag                                                                                                                                         <  6:24563/133‑3 (MQ=255)                       aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGcag                                                                                                                                         >  5:24563/1‑131 (MQ=255)                           aTCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTg                                                                                                                     <  5:151786/149‑1 (MQ=255)                            tCGGCCTGGCGCGAGCGTTTGGCCGGGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTgg                                                                                                                    <  3:90018/149‑1 (MQ=255)                               gCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGca                                                                                                                 >  8:212233/1‑149 (MQ=255)                                   ggCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAgg                                                                                                             >  7:272305/1‑149 (MQ=255)                                   ggCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAgg                                                                                                             >  1:299521/1‑149 (MQ=255)                                    gcgcGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTTGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAg                                                                                                              <  3:67814/147‑1 (MQ=255)                                         aGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTAcc                                                                                                       <  1:241801/149‑1 (MQ=255)                                                  ccGCGAGGTGATTGCCGAGGGGATGGAGAGTGTCTAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTTCTGCATCGCCAGGCCGATGCCCGCCAGTGACGTGCACGCCTGGGCACAGGACGACCAGCAGCcag                                                                                              >  8:194576/1‑149 (MQ=255)                                                    gcgAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtg                                                                                            <  3:52975/149‑1 (MQ=255)                                                      gAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtgca                                                                                          <  4:78020/149‑1 (MQ=255)                                                        ggTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCcagtgcagt                                                                                        >  3:17592/1‑149 (MQ=255)                                                            aTTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGc                                                                                     <  6:384511/148‑1 (MQ=255)                                                             ttGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGt                                                                                   <  8:217159/149‑1 (MQ=255)                                                              tGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGt                                                                                   <  4:196770/148‑1 (MQ=255)                                                                 cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAcc                                                                               >  1:353675/1‑149 (MQ=255)                                                                 cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAcc                                                                               >  2:77320/1‑149 (MQ=255)                                                                           gagaGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGtc                                                                     <  2:28066/149‑1 (MQ=255)                                                                           gagaGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGtc                                                                     <  2:179806/149‑1 (MQ=255)                                                                                  tCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCg                                                              <  5:165816/149‑1 (MQ=255)                                                                                    gatcACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCTATGCGCGCCAGTGACGTGCTAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCggg                                                            <  7:250925/146‑1 (MQ=255)                                                                                     aGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGc                                                           >  5:364872/1‑149 (MQ=255)                                                                                       cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACcagcag                                                                                                  >  4:37355/1‑108 (MQ=255)                                                                                       cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACcagcag                                                                                                  <  3:37355/108‑1 (MQ=255)                                                                                       cACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCg                                                         >  7:26232/1‑149 (MQ=255)                                                                                               ggTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCtt                                                  >  8:365077/1‑148 (MQ=255)                                                                                               ggTACGGATGGGCCTTGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTg                                                 <  4:14882/149‑1 (MQ=255)                                                                                                gTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                       >  4:188130/1‑142 (MQ=255)                                                                                                gTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                       <  3:188130/142‑1 (MQ=255)                                                                                                 tACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGAc                                               >  4:173013/1‑149 (MQ=255)                                                                                                      aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                       <  5:78415/136‑1 (MQ=255)                                                                                                      aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                                                       >  6:78415/1‑136 (MQ=255)                                                                                                      aTGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCa                                          >  6:96725/1‑149 (MQ=255)                                                                                                          gCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACggg                                      <  1:77320/149‑1 (MQ=255)                                                                                                             tGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACgggggg                                   >  3:97502/1‑149 (MQ=255)                                                                                                             tGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACgggggg                                   >  6:307867/1‑149 (MQ=255)                                                                                                                  tGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCa                              >  5:250930/1‑149 (MQ=255)                                                                                                                   gCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCAc                             <  3:219203/149‑1 (MQ=255)                                                                                                                   gCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCAc                             <  7:251922/149‑1 (MQ=255)                                                                                                                          ggCGCAGGGTTACTGAATAGCCAGGACGATGCCCGCAAGTGACGAGCAATACTGGGCACAGGCCTACCAGAACACACTCAACTCCCGAGACACATGAAGGTCATCAGCGGGCCGTCGACTTGCCCTCAACGCCGGGCGCAACCTGCTTg                      >  6:248314/1‑149 (MQ=255)                                                                                                                           gcgcAGGGTTACTGCTTCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGGCTGGGCAGAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGGGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCCTGt                     <  2:203408/149‑1 (MQ=255)                                                                                                                           gcgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGt                     <  8:26232/149‑1 (MQ=255)                                                                                                                           gcgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCGGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCtt                       <  7:6092/147‑1 (MQ=255)                                                                                                                            cgcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                    <  4:364929/149‑1 (MQ=255)                                                                                                                             gcAGGGTTACTGCATCGCTCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                    >  8:233613/1‑148 (MQ=255)                                                                                                                             gcAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTc                    >  7:143281/1‑148 (MQ=255)                                                                                                                               aGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGAt                 <  4:12064/149‑1 (MQ=255)                                                                                                                               aGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGAt                 <  4:345830/149‑1 (MQ=255)                                                                                                                                gggTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATc                <  6:364872/149‑1 (MQ=255)                                                                                                                                gggTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATc                <  4:97502/149‑1 (MQ=255)                                                                                                                                  gTTACTGCATCGCCCGTCCGATGCACGCCAGTGACGTGCTAGCGTGGGCGCAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAGCCTTGAAGGTCATCAGCGGTGCGTGGTCTTGACCTGCGCTGGGGGCGCCACAGTCTAGTGGATCAg              >  4:82268/1‑149 (MQ=255)                                                                                                                                     aCTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAggg            <  7:365077/148‑1 (MQ=255)                                                                                                                                      gggCATCGGCCGGGCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGt          >  6:239155/3‑149 (MQ=255)                                                                                                                                      gggCATCGGCCGGGCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGt          >  3:372576/3‑149 (MQ=255)                                                                                                                                         cATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTg         <  8:143281/147‑1 (MQ=255)                                                                                                                                          aTCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCt      >  8:83592/1‑149 (MQ=255)                                                                                                                                            ctcccGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTc    <  7:194576/147‑1 (MQ=255)                                                                                                                                             gCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCg   <  2:299521/149‑1 (MQ=255)                                                                                                                                               ccGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCGa  >  7:93224/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                ACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTGCTTGTCGATCAGGGGTGGCTTCGA  >  NC_002947/2576295‑2576584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑