Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,971 G→A 11.7% P179S (CCG→TCG)  PP_2403 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,9710GA11.7% 133.3 / 7.5 51P179S (CCG→TCG) PP_2403chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base A (4/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

ACGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTT  >  NC_002947/2747823‑2748118
                                                                                                                                                            |                                                                                                                                                   
aCGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCgg                                                                                                                                                     <  4:276352/149‑1 (MQ=255)
aCGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCgg                                                                                                                                                     <  2:158139/149‑1 (MQ=255)
        gATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGGGGTGGTAGAGGTTCGGGAATCGCAGCCCTGCGTCATGTTAAGCAGCTCTTCGCTGCTGAGCACCCGCTGCgg                                                                                                                                                     >  1:320041/1‑149 (MQ=255)
        gATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTc                                                                                                                                               >  1:149527/1‑147 (MQ=255)
               cTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAacac                                                                                                                                      >  4:287443/1‑149 (MQ=255)
                  ggCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCg                                                                                                                                   <  1:151833/149‑1 (MQ=255)
                  ggCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCg                                                                                                                                   <  2:149527/149‑1 (MQ=255)
                    cTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgca                                                                                                                                 <  3:327702/149‑1 (MQ=255)
                     tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAaca                                                                                                                                       <  8:156972/142‑1 (MQ=255)
                     tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAaca                                                                                                                                       >  7:156972/1‑142 (MQ=255)
                       gcgGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               <  8:240588/148‑1 (MQ=255)
                       gcgGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCACCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATT‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCTCGCCTGAGCACCCGCTGCGGGTGGTAGAGGAACACTCgcag                                                                                                                                >  2:379871/1‑147 (MQ=255)
                         gggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAgg                                                                                                                            <  8:7627/149‑1 (MQ=255)
                             aTCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCgg                                                                                                                         >  4:171281/1‑148 (MQ=255)
                                gTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACt                                                                                                                     <  2:59573/149‑1 (MQ=255)
                                gTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTAGCGGCCCTGGGTCAGGTTAAGCAGCTGTTAGCGGCTGAGCCCCCCCTGCGGGTGGTAGAGGAACACTCGCAGCAGGCGGTACt                                                                                                                     >  1:225487/1‑149 (MQ=255)
                                    cccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgc                                                                                                                 >  5:177491/1‑149 (MQ=255)
                                     ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                >  5:323980/1‑149 (MQ=255)
                                      cAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                >  4:324339/1‑148 (MQ=255)
                                      cAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                <  3:324339/148‑1 (MQ=255)
                                         gcgcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGcgccgc                                                                                                            >  1:188315/1‑149 (MQ=255)
                                           gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTc                                                                                                          <  5:10129/149‑1 (MQ=255)
                                             tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               >  3:230666/1‑126 (MQ=255)
                                             tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               <  4:230666/126‑1 (MQ=255)
                                               gcgcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAg                                                                                                        <  2:320041/147‑1 (MQ=255)
                                                 gcAGGCGGTTGCCCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTAAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcc                                                                                                    >  7:151355/1‑149 (MQ=255)
                                                         cTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGt                                                                                            <  3:287443/149‑1 (MQ=255)
                                                         cTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGt                                                                                            <  7:241147/149‑1 (MQ=255)
                                                                    gTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGt                                                                                 >  4:150228/1‑149 (MQ=255)
                                                                    gTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGt                                                                                 <  2:188315/149‑1 (MQ=255)
                                                                       gATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTc                                                                                >  4:70355/1‑147 (MQ=255)
                                                                            aGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAgg                                                                         >  1:275306/1‑149 (MQ=255)
                                                                              cGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTg                                                                       <  6:177491/149‑1 (MQ=255)
                                                                                 tC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGgcg                                                                    >  4:245785/1‑149 (MQ=255)
                                                                                             aTG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggt                                                              >  5:227963/1‑149 (MQ=255)
                                                                                                 tC‑GGCTTCGCGGCCCTGGGTCATGTTAAGCAGCTGGTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGGAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtggt                                                           <  3:104376/149‑1 (MQ=255)
                                                                                                 tC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtggt                                                           >  6:18752/1‑149 (MQ=255)
                                                                                                    ggCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGgtgt                                                         <  6:323980/149‑1 (MQ=255)
                                                                                                          gcgGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAAGACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCAGTCGAGCAGGTGGCTCGCCGTGGTGTCCAGgc                                                   >  7:168841/1‑149 (MQ=255)
                                                                                                               ccTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCAt                                              >  4:358498/1‑149 (MQ=255)
                                                                                                                   ggTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAc                                          >  5:353119/1‑149 (MQ=255)
                                                                                                                   ggTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAc                                          >  4:329261/1‑149 (MQ=255)
                                                                                                                        ggTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGaaa                                     <  6:227963/149‑1 (MQ=255)
                                                                                                                         gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                                    >  1:363421/1‑149 (MQ=255)
                                                                                                                         gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGCTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGCTGGTGTCCAGGCGCCATTGACCGAAAc                                    >  1:311376/1‑149 (MQ=255)
                                                                                                                            aaGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAccc                                  <  8:214277/148‑1 (MQ=255)
                                                                                                                              gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcagc                               <  3:245785/149‑1 (MQ=255)
                                                                                                                              gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcag                                >  4:372786/1‑148 (MQ=255)
                                                                                                                                         cgGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGgctgct                    <  6:353119/149‑1 (MQ=255)
                                                                                                                                                   cctgCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg          >  6:237027/4‑149 (MQ=255)
                                                                                                                                                   cccGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg          >  4:367493/1‑149 (MQ=255)
                                                                                                                                                      gCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGt        >  5:44196/1‑148 (MQ=255)
                                                                                                                                                      gCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGt        >  3:370229/1‑148 (MQ=255)
                                                                                                                                                          cGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAg     <  7:291992/147‑1 (MQ=255)
                                                                                                                                                           gggTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGtt  <  2:275306/149‑1 (MQ=255)
                                                                                                                                                           gggTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGtt  <  1:379871/149‑1 (MQ=255)
                                                                                                                                                            |                                                                                                                                                   
ACGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTC‑‑‑‑‑‑GAAGATG‑TC‑GGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTT  >  NC_002947/2747823‑2748118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑