Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,975 G→T 11.3% D177E (GAC→GAA)  PP_2403 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,9750GT11.3% 143.4 / 10.5 53D177E (GAC→GAA) PP_2403chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base T (4/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

GATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTT  >  NC_002947/2747831‑2748118
                                                                                                                                                |                                                                                                                                               
gATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTc                                                                                                                                               >  1:149527/1‑147 (MQ=255)
       cTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAacac                                                                                                                                      >  4:287443/1‑149 (MQ=255)
          ggCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCg                                                                                                                                   <  1:151833/149‑1 (MQ=255)
          ggCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCg                                                                                                                                   <  2:149527/149‑1 (MQ=255)
            cTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgca                                                                                                                                 <  3:327702/149‑1 (MQ=255)
             tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAaca                                                                                                                                       <  8:156972/142‑1 (MQ=255)
             tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAaca                                                                                                                                       >  7:156972/1‑142 (MQ=255)
               gcgGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               <  8:240588/148‑1 (MQ=255)
               gcgGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCACCAAGTCGATGGAGCGGTCGAAGATTTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCTCGCCTGAGCACCCGCTGCGGGTGGTAGAGGAACACTCgcag                                                                                                                                >  2:379871/1‑147 (MQ=255)
                 gggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAgg                                                                                                                            <  8:7627/149‑1 (MQ=255)
                     aTCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCgg                                                                                                                         >  4:171281/1‑148 (MQ=255)
                        gTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACt                                                                                                                     <  2:59573/149‑1 (MQ=255)
                        gTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTAGCGGCCCTGGGTCAGGTTAAGCAGCTGTTAGCGGCTGAGCCCCCCCTGCGGGTGGTAGAGGAACACTCGCAGCAGGCGGTACt                                                                                                                     >  1:225487/1‑149 (MQ=255)
                            cccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgc                                                                                                                 >  5:177491/1‑149 (MQ=255)
                             ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                >  5:323980/1‑149 (MQ=255)
                              cAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                <  3:324339/148‑1 (MQ=255)
                              cAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGgcg                                                                                                                >  4:324339/1‑148 (MQ=255)
                                 gcgcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGcgccgc                                                                                                            >  1:188315/1‑149 (MQ=255)
                                   gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTc                                                                                                          <  5:10129/149‑1 (MQ=255)
                                     tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               >  3:230666/1‑126 (MQ=255)
                                     tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGAGTGTTCCTCGACCACCCgcagc                                                                                                                               <  4:230666/126‑1 (MQ=255)
                                       gcgcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAg                                                                                                        <  2:320041/147‑1 (MQ=255)
                                         gcAGGCGGTTGCCCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTAAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcc                                                                                                    >  7:151355/1‑149 (MQ=255)
                                                 cTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGt                                                                                            <  7:241147/149‑1 (MQ=255)
                                                 cTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGt                                                                                            <  3:287443/149‑1 (MQ=255)
                                                            gTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGt                                                                                 >  4:150228/1‑149 (MQ=255)
                                                            gTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGt                                                                                 <  2:188315/149‑1 (MQ=255)
                                                               gATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTc                                                                                >  4:70355/1‑147 (MQ=255)
                                                                    aGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAgg                                                                         >  1:275306/1‑149 (MQ=255)
                                                                      cGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTg                                                                       <  6:177491/149‑1 (MQ=255)
                                                                         tCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGgcg                                                                    >  4:245785/1‑149 (MQ=255)
                                                                               aTGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggt                                                              >  5:227963/1‑149 (MQ=255)
                                                                                  tCGGCTTCGCGGCCCTGGGTCATGTTAAGCAGCTGGTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGGAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtggt                                                           <  3:104376/149‑1 (MQ=255)
                                                                                  tCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtggt                                                           >  6:18752/1‑149 (MQ=255)
                                                                                    ggCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGgtgt                                                         <  6:323980/149‑1 (MQ=255)
                                                                                          gcgGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAAGACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCAGTCGAGCAGGTGGCTCGCCGTGGTGTCCAGgc                                                   >  7:168841/1‑149 (MQ=255)
                                                                                               ccTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCAt                                              >  4:358498/1‑149 (MQ=255)
                                                                                                   ggTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAc                                          >  4:329261/1‑149 (MQ=255)
                                                                                                   ggTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAc                                          >  5:353119/1‑149 (MQ=255)
                                                                                                        ggTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGaaa                                     <  6:227963/149‑1 (MQ=255)
                                                                                                         gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                                    >  1:363421/1‑149 (MQ=255)
                                                                                                         gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGCTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGCTGGTGTCCAGGCGCCATTGACCGAAAc                                    >  1:311376/1‑149 (MQ=255)
                                                                                                            aaGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAccc                                  <  8:214277/148‑1 (MQ=255)
                                                                                                              gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcagc                               <  3:245785/149‑1 (MQ=255)
                                                                                                              gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcag                                >  4:372786/1‑148 (MQ=255)
                                                                                                                         cgGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGgctgct                    <  6:353119/149‑1 (MQ=255)
                                                                                                                                   cctgCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg          >  6:237027/4‑149 (MQ=255)
                                                                                                                                   cccGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg          >  4:367493/1‑149 (MQ=255)
                                                                                                                                      gCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGt        >  3:370229/1‑148 (MQ=255)
                                                                                                                                      gCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGt        >  5:44196/1‑148 (MQ=255)
                                                                                                                                          cGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAg     <  7:291992/147‑1 (MQ=255)
                                                                                                                                           gggTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGtt  <  1:379871/149‑1 (MQ=255)
                                                                                                                                           gggTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGtt  <  2:275306/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTT  >  NC_002947/2747831‑2748118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑