Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,883 A→C 12.9% E70A (GAG→GCG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,8830AC12.9% 134.9 / 11.7 53E70A (GAG→GCG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base C (4/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGA  >  NC_002947/2753739‑2754030
                                                                                                                                                |                                                                                                                                                   
tGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGAcc                                                                                                                                                 >  3:184696/1‑149 (MQ=255)
       cgcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCga                                                                                                                                                     <  2:134907/138‑1 (MQ=255)
           gTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                      <  5:319837/149‑4 (MQ=38)
           gTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtggctgg                                                                                                                                      <  3:90985/149‑1 (MQ=255)
           aagggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                      <  3:183816/147‑4 (MQ=37)
             gggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGcc                                                                                                                                    >  7:56084/1‑149 (MQ=255)
              ggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                   >  2:117075/1‑149 (MQ=255)
                tATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCTGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                 >  5:328855/1‑149 (MQ=255)
                   ccACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAg                                                                                                                              >  3:192891/1‑149 (MQ=255)
                     acaGCTACCAGAAACTGCCGATCGATGCTGTGCCTGACATCACTAACTTCCAGGTGCAGATCAACACCTCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                            <  8:324198/149‑1 (MQ=255)
                         cTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCACCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCa                                                                                                                        <  1:80994/149‑1 (MQ=255)
                            ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                      >  2:236339/1‑148 (MQ=255)
                             cAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                      >  6:238925/1‑147 (MQ=255)
                             cAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATAAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                      >  1:122597/1‑147 (MQ=255)
                                       cgatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                            <  7:52926/131‑1 (MQ=255)
                                       cgatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                            >  8:52926/1‑131 (MQ=255)
                                         atcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCAAGGGCTCAAGCAGACCcgctcgct                                                                                                        >  2:97864/1‑149 (MQ=255)
                                              tGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTcgc                                                                                                   >  5:146833/1‑149 (MQ=255)
                                                 tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGACATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCt                                                                                                >  5:167515/1‑149 (MQ=255)
                                                  gtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTc                                                                                               >  1:50824/1‑149 (MQ=255)
                                                     cctgACATCACTAACGTCCATGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCggg                                                                                            <  5:278881/146‑1 (MQ=255)
                                                     ccGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCgg                                                                                             <  4:184696/148‑1 (MQ=255)
                                                       ggACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGCCCGAGCAGCGCATCGCCTTTGCCGTCGCGCCGGCGATGGCTGGCCGGCCGGGGCGGGAGCGGTCCCGCTCGCGGTCGGGCGCGGGcg                                                                                          >  4:278214/1‑148 (MQ=255)
                                                       ggACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGcc                                                                                          >  2:5169/1‑149 (MQ=255)
                                                            tCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTgctt                                                                                     <  2:200815/149‑4 (MQ=255)
                                                             cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg                                                                                     >  1:113942/1‑148 (MQ=255)
                                                             cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg                                                                                     >  5:348568/1‑148 (MQ=255)
                                                              aCTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                 >  1:69794/1‑103 (MQ=255)
                                                              aCTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                 <  2:69794/103‑1 (MQ=255)
                                                               cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                  <  4:192891/149‑1 (MQ=255)
                                                               cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                  <  8:56084/149‑1 (MQ=255)
                                                                  aCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTc                                                                               <  1:163596/149‑1 (MQ=255)
                                                                  aCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTc                                                                               <  5:238925/149‑1 (MQ=255)
                                                                                  cAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCGAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgat                                                                >  4:32086/1‑148 (MQ=255)
                                                                                        cgccgcCCCTGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCa                                                         >  3:8152/1‑149 (MQ=255)
                                                                                         gccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCAc                                                        <  3:278214/149‑1 (MQ=255)
                                                                                         gccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCAc                                                        <  1:5169/149‑1 (MQ=255)
                                                                                                   gCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttc                                              <  6:348568/149‑1 (MQ=255)
                                                                                                    cTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGGCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCTCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttct                                             <  6:349861/149‑1 (MQ=255)
                                                                                                          gccgcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCGCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgccc                                       <  8:341182/149‑1 (MQ=255)
                                                                                                                    aCCGAGCAGCGCATCACCTTTGACATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGt                             <  6:167515/149‑1 (MQ=255)
                                                                                                                          cagcGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGa                       <  3:168924/149‑1 (MQ=255)
                                                                                                                             cgcATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgcg                    >  5:113139/1‑149 (MQ=255)
                                                                                                                              gcATCACCTTTCCCATCGAGCCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgcgc                   <  6:284252/149‑1 (MQ=255)
                                                                                                                                     cTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGTCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAgg            <  1:97864/149‑1 (MQ=255)
                                                                                                                                      ccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt           >  6:187783/4‑149 (MQ=255)
                                                                                                                                      ccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt           >  7:258806/4‑149 (MQ=255)
                                                                                                                                       cagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCACATGGTCAACGAGCGCCTGCAGGTg          >  7:352042/3‑149 (MQ=255)
                                                                                                                                        agCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTgg         <  5:187783/148‑1 (MQ=255)
                                                                                                                                         gCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCGGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCGCGCCAACTGGGCAGCGCGCGCCTGCAGGTgga        >  6:266618/1‑148 (MQ=255)
                                                                                                                                           cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGat                                                    >  4:246853/1‑103 (MQ=255)
                                                                                                                                           cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGat                                                    <  3:246853/103‑1 (MQ=255)
                                                                                                                                            aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg     <  6:328855/149‑1 (MQ=255)
                                                                                                                                             tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcgc    >  5:1998/1‑149 (MQ=255)
                                                                                                                                               gagaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGa  <  2:50824/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGA  >  NC_002947/2753739‑2754030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑