Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,886 C→T 10.9% T71I (ACC→ATC) ‡ czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,8860CT10.9% 143.5 / 7.2 55T71I (ACC→ATC) ‡czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base T (3/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGC  >  NC_002947/2753739‑2754032
                                                                                                                                                   |                                                                                                                                                  
tGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGAcc                                                                                                                                                   >  3:184696/1‑149 (MQ=255)
           gTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                        <  5:319837/149‑4 (MQ=38)
           gTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtggctgg                                                                                                                                        <  3:90985/149‑1 (MQ=255)
           aagggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                        <  3:183816/147‑4 (MQ=37)
             gggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGcc                                                                                                                                      >  7:56084/1‑149 (MQ=255)
              ggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                     >  2:117075/1‑149 (MQ=255)
                tATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCTGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                   >  5:328855/1‑149 (MQ=255)
                   ccACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAg                                                                                                                                >  3:192891/1‑149 (MQ=255)
                     acaGCTACCAGAAACTGCCGATCGATGCTGTGCCTGACATCACTAACTTCCAGGTGCAGATCAACACCTCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                              <  8:324198/149‑1 (MQ=255)
                         cTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCACCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCa                                                                                                                          <  1:80994/149‑1 (MQ=255)
                            ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                        >  2:236339/1‑148 (MQ=255)
                             cAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                        >  6:238925/1‑147 (MQ=255)
                             cAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATAAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                        >  1:122597/1‑147 (MQ=255)
                                       cgatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                              >  8:52926/1‑131 (MQ=255)
                                       cgatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                              <  7:52926/131‑1 (MQ=255)
                                         atcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCAAGGGCTCAAGCAGACCcgctcgct                                                                                                          >  2:97864/1‑149 (MQ=255)
                                              tGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTcgc                                                                                                     >  5:146833/1‑149 (MQ=255)
                                                 tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGACATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCt                                                                                                  >  5:167515/1‑149 (MQ=255)
                                                  gtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTc                                                                                                 >  1:50824/1‑149 (MQ=255)
                                                     cctgACATCACTAACGTCCATGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCggg                                                                                              <  5:278881/146‑1 (MQ=255)
                                                     ccGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCgg                                                                                               <  4:184696/148‑1 (MQ=255)
                                                       ggACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGCCCGAGCAGCGCATCGCCTTTGCCGTCGCGCCGGCGATGGCTGGCCGGCCGGGGCGGGAGCGGTCCCGCTCGCGGTCGGGCGCGGGcg                                                                                            >  4:278214/1‑148 (MQ=255)
                                                       ggACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGcc                                                                                            >  2:5169/1‑149 (MQ=255)
                                                            tCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTgctt                                                                                       <  2:200815/149‑4 (MQ=255)
                                                             cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg                                                                                       >  5:348568/1‑148 (MQ=255)
                                                             cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg                                                                                       >  1:113942/1‑148 (MQ=255)
                                                              aCTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                   <  2:69794/103‑1 (MQ=255)
                                                              aCTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGc                                                                                                                                   >  1:69794/1‑103 (MQ=255)
                                                               cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                    <  8:56084/149‑1 (MQ=255)
                                                               cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                    <  4:192891/149‑1 (MQ=255)
                                                                  aCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTc                                                                                 <  1:163596/149‑1 (MQ=255)
                                                                  aCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTc                                                                                 <  5:238925/149‑1 (MQ=255)
                                                                                  cAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCGAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgat                                                                  >  4:32086/1‑148 (MQ=255)
                                                                                        cgccgcCCCTGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCa                                                           >  3:8152/1‑149 (MQ=255)
                                                                                         gccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCAc                                                          <  1:5169/149‑1 (MQ=255)
                                                                                         gccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCAc                                                          <  3:278214/149‑1 (MQ=255)
                                                                                                   gCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttc                                                <  6:348568/149‑1 (MQ=255)
                                                                                                    cTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGGCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCTCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttct                                               <  6:349861/149‑1 (MQ=255)
                                                                                                          gccgcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCGCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgccc                                         <  8:341182/149‑1 (MQ=255)
                                                                                                                    aCCGAGCAGCGCATCACCTTTGACATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGt                               <  6:167515/149‑1 (MQ=255)
                                                                                                                          cagcGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGa                         <  3:168924/149‑1 (MQ=255)
                                                                                                                             cgcATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgcg                      >  5:113139/1‑149 (MQ=255)
                                                                                                                              gcATCACCTTTCCCATCGAGCCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgcgc                     <  6:284252/149‑1 (MQ=255)
                                                                                                                                     cTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGTCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAgg              <  1:97864/149‑1 (MQ=255)
                                                                                                                                      ccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt             >  6:187783/4‑149 (MQ=255)
                                                                                                                                      ccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt             >  7:258806/4‑149 (MQ=255)
                                                                                                                                       cagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCACATGGTCAACGAGCGCCTGCAGGTg            >  7:352042/3‑149 (MQ=255)
                                                                                                                                        agCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTgg           <  5:187783/148‑1 (MQ=255)
                                                                                                                                         gCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCGGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCGCGCCAACTGGGCAGCGCGCGCCTGCAGGTgga          >  6:266618/1‑148 (MQ=255)
                                                                                                                                           cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGat                                                      <  3:246853/103‑1 (MQ=255)
                                                                                                                                           cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGat                                                      >  4:246853/1‑103 (MQ=255)
                                                                                                                                            aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg       <  6:328855/149‑1 (MQ=255)
                                                                                                                                             tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcgc      >  5:1998/1‑149 (MQ=255)
                                                                                                                                               gagaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGa    <  2:50824/149‑1 (MQ=255)
                                                                                                                                                  aCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGc  >  6:69604/1‑148 (MQ=255)
                                                                                                                                                  aCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGc  >  4:63908/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGC  >  NC_002947/2753739‑2754032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑