Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	18,554	A→T	5.2%	R449S (AGA→AGT)	PP_0014 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	18,554	0	A	T	5.2%	226.4 / 2.7	77	R449S (AGA→AGT)	PP_0014	transposase
Reads supporting (aligned to +/- strand):  ref base A (38/34);  new base T (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

CCACCTTCTCGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTGGGCTAC  >  NC_002947/18412‑18698                                                                                                                                               |                                                                                                                                                  ccaccTTCTCGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCg                                                                                                                                             <  2:335176/149‑1 (MQ=255)  caccTTCTCGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCgg                                                                                                                                            >  4:277235/1‑149 (MQ=255)  caccTTCTCGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCgg                                                                                                                                            >  8:61712/1‑149 (MQ=255)       tctcGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑Tc                                                                                                                                        >  1:184760/1‑147 (MQ=255)        ctcGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGACTGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTGCCACCAGACTCCTCATCGGGGGCTGGACATCAGACCACCGGC‑TCAAg                                                                                                                                     >  2:232129/1‑149 (MQ=255)           gAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCt                                                                                                                                  >  6:175756/1‑149 (MQ=255)           gAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGCATAGTCGATGGTAACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACAACGGC‑TAATGTCt                                                                                                                                  >  1:153227/1‑149 (MQ=255)             atatCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTgg                                                                                                                                <  6:147217/149‑1 (MQ=255)              tatCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCaa                                                                                                                                      <  4:220475/142‑1 (MQ=255)              tatCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCaa                                                                                                                                      >  3:220475/1‑142 (MQ=255)              tatCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTggg                                                                                                                               >  3:21101/1‑149 (MQ=255)              tatCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTggg                                                                                                                               <  4:21101/149‑1 (MQ=255)              tatCCAGCAGCGTGGCGAGTACGACTCGGACAAGAATGCATGGCTCACGCTCCCTCACCTGGAGGCGGCGGTAGTGAAATGGACCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑GCAAGACTggg                                                                                                                               <  4:119645/149‑1 (MQ=255)                tCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTgggg                                                                                                                              <  3:277235/148‑1 (MQ=255)                  cagcagCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGa                                                                                                                            <  1:2043/148‑1 (MQ=255)                    gcagcGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGAc                                                                                                                           >  3:376489/1‑147 (MQ=255)                        cGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGa                                                                                                                     <  2:55283/149‑1 (MQ=255)                         gTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAg                                                                                                                    <  3:124588/149‑1 (MQ=255)                               aTTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAAc                                                                                                              <  4:139132/149‑1 (MQ=255)                                 tACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAAGAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCa                                                                                                            >  5:332292/1‑149 (MQ=255)                                      cTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGtca                                                                                                       >  3:318146/1‑149 (MQ=255)                                      cTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCGGGCCAACAGACCACCGGC‑TCAAGTAGGGGGGACTAGCGAGGCGCACCATGt                                                                                                         >  4:259055/1‑147 (MQ=255)                                         ggAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGAGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACCCCGGC‑TAAAGTCTGGGGGACTAGCGAGGCGAACCATGtcatc                                                                                                     >  8:275299/1‑148 (MQ=255)                                          gagaAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCaa                                                                                                                                      >  5:178268/1‑114 (MQ=255)                                          gagaAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCaa                                                                                                                                      <  6:178268/114‑1 (MQ=255)                                               gaaTGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCTCCAGCCTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCGGGGGGACTAGCGAGGCGAACCATGTCAGCAGCCTTc                                                                                              >  8:100881/1‑149 (MQ=255)                                               gaaTGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTc                                                                                              <  4:167004/149‑1 (MQ=255)                                                  tGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCg                                                                                           >  8:80265/1‑149 (MQ=255)                                                     aTGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGTACACCGGC‑t                                                                                                                                         <  6:283634/100‑1 (MQ=255)                                                     aTGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGTACACCGGC‑t                                                                                                                                         <  6:278403/100‑1 (MQ=255)                                                     aTGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGTACACCGGC‑t                                                                                                                                         >  5:283634/1‑100 (MQ=255)                                                     aTGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGTACACCGGC‑t                                                                                                                                         >  5:278403/1‑100 (MQ=255)                                                          tCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGCAGACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAGCCATGTCATGATCATTCCCGTCGCCCTg                                                                                   >  4:62204/1‑149 (MQ=255)                                                            aCGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGa                                                                                 >  6:76992/1‑149 (MQ=255)                                                              gCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACt                                                                               >  7:364403/1‑149 (MQ=255)                                                                tCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCg                                                                             >  3:284390/1‑149 (MQ=255)                                                                  ccTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGtt                                                                           <  7:80265/149‑1 (MQ=255)                                                                  ccTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGCCGAACCATGTCATCAGCATTCCCGTCGACCTGGAATCGtt                                                                           >  8:279613/1‑149 (MQ=255)                                                                   cTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGCCTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTg                                                                          >  4:52041/1‑149 (MQ=255)                                                                   cTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTg                                                                          >  6:46751/1‑149 (MQ=255)                                                                   cTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGGCGACGGGGACTCGTTg                                                                          >  4:213395/1‑149 (MQ=255)                                                                      aaTTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGa                                                                        <  8:364403/148‑1 (MQ=255)                                                                      aaTTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGa                                                                        <  6:332292/148‑1 (MQ=255)                                                                      aaTTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGa                                                                        <  2:184760/148‑1 (MQ=255)                                                                       aTTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGt                                                                      <  7:70117/149‑1 (MQ=255)                                                                       aTTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGt                                                                      >  8:236062/1‑149 (MQ=255)                                                                                ggtggtAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTgg                                                             >  7:297590/1‑149 (MQ=255)                                                                                  tggtAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGcc                                                           <  4:318146/149‑1 (MQ=255)                                                                                    gtAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCaa                                                         <  4:284390/149‑1 (MQ=255)                                                                                       gTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCg                                                      >  5:25498/1‑149 (MQ=255)                                                                                             tGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTc                                                <  5:175756/149‑1 (MQ=255)                                                                                                    tcgATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGa                                                                        >  5:197858/1‑118 (MQ=255)                                                                                                    tcgATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGa                                                                        <  6:197858/118‑1 (MQ=255)                                                                                                       aTGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGCGTCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGt                                       >  1:285683/1‑149 (MQ=255)                                                                                                       aTGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGtt                                      <  4:376489/149‑1 (MQ=255)                                                                                                        tGGCTACCACCAGACTCCTCATCGGGGGCCGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCGTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGGTc                                     <  4:252800/149‑1 (MQ=255)                                                                                                          gTTAACACCAGACTCCTCATCGGGTGCTGGAAAACAGAACACCGGC‑TCAAGGCTGTGGGACTAGAGAGGCTAAACATGTCATGAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCAc                                   <  7:336916/149‑1 (MQ=255)                                                                                                               caccaGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTa                              >  1:207353/1‑149 (MQ=255)                                                                                                                   aGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACgg                           >  2:204197/1‑148 (MQ=255)                                                                                                                            aTCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGtt                 >  5:297574/1‑149 (MQ=255)                                                                                                                             tCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTg                <  6:25498/149‑1 (MQ=255)                                                                                                                             tCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTg                <  2:207353/149‑1 (MQ=255)                                                                                                                             tCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTg                >  6:125916/1‑149 (MQ=255)                                                                                                                                  ggCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCaaa           >  1:323660/1‑149 (MQ=255)                                                                                                                                   gCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAg          >  3:313581/1‑149 (MQ=255)                                                                                                                                    cTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGt         <  6:297574/149‑1 (MQ=255)                                                                                                                                       gACAACAGAACACCGGC‑TCAATTCTGGGGGACTAGCGAGGCGAACCATGGCATCAGCCTTCCCGTCGACCTTGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTgg       <  7:275299/148‑1 (MQ=255)                                                                                                                                       gACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTg        <  7:279613/147‑1 (MQ=255)                                                                                                                                       gACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTg        <  3:259055/147‑1 (MQ=255)                                                                                                                                       gACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCCAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTgg       <  2:306034/148‑1 (MQ=255)                                                                                                                                          aacaGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTGGGCTa   >  1:8263/1‑149 (MQ=255)                                                                                                                                          aacaGAACACAGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACt                                                                               >  3:114000/1‑73 (MQ=255)                                                                                                                                          aacaGAACACAGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACt                                                                               <  4:114000/73‑1 (MQ=255)                                                                                                                                           acaGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTGGGCTAc  <  7:236062/149‑1 (MQ=255)                                                                                                                                            caGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCa                                    >  2:238513/1‑114 (MQ=255)                                                                                                                                            caGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCa                                    <  1:238513/114‑1 (MQ=255)                                                                                                                                            caGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTGGGCTAc  >  4:365915/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                  CCACCTTCTCGAATATCCAGCAGCGTGGCGATTACGACTCGGAGAAGAATGCATGTTTCACGCTCCCTCAATTGGAGGCGGTGGTAGTGAAATGGATCGTCGATGGTTACCACCAGACTCCTCATCGGGGGCTGGACAACAGAACACCGGC‑TCAAGTCTGGGGGACTAGCGAGGCGAACCATGTCATCAGCCTTCCCGTCGACCTGGACTCGTTGGAGTGCATTCTGGCCAAGCGGGCCTCGGTGAAGGTTCACCATTACGGCATCGAGGTTGCCAAAGTGGGCTAC  >  NC_002947/18412‑18698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑