Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,864,700 T→A 5.6% Y228F (TAC→TTC)  gllP ← porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,864,7000TA 5.6% 219.1 / 2.4 72Y228F (TAC→TTC) gllPporin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base A (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

GCCGAGAACAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGCGTCGTTGCGCG  >  NC_002947/2864553‑2864847
                                                                                                                                                    |                                                                                                                                                    
gCCGAGAACAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGt                                                                                                                                                      <  6:164590/148‑1 (MQ=255)
  cGAGAACAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGa                                                                                                                                                   <  3:60766/149‑1 (MQ=255)
   gagaACAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGAt                                                                                                                                                  <  4:130546/149‑1 (MQ=255)
        cAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCtt                                                                                                                                             <  8:12226/149‑1 (MQ=255)
         aGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTc                                                                                                                                            >  4:162223/1‑149 (MQ=255)
                aaGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTgg                                                                                                                                     <  5:367822/149‑1 (MQ=255)
                 aGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGACC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTggg                                                                                                                                    <  8:55720/149‑1 (MQ=255)
                 aGCGGTGCGGTTGTTAAGTTCGCCTGCCTTGGGGGCACCGTCGTCCTTGCCCCACTAGTATCCCAGGTTTGCGCCCAGTACCCAGTTGCCCTTGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGAAGACATCTACCAGcagc                                                                                                                                     <  1:106744/149‑4 (MQ=255)
                      tgcggtTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATAc                                                                                                                               <  2:313360/149‑1 (MQ=255)
                          gttgttCAGTTCGCCTGCCTTGGCTCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTGGGCGCCCAGTACCCAGTTGCAC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATAc                                                                                                                               <  7:181160/145‑1 (MQ=255)
                          gttgttCAGTTCGCCTGCCTTGGCGCCACAGTCGTCCTTGCCCCAGAAGTAGACCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCAGGTAGATGTATTCCAGCTGGGCATaa                                                                                                                               >  8:181160/1‑144 (MQ=255)
                              ttCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGcc                                                                                                                       >  6:319093/1‑149 (MQ=255)
                              ttCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGGAGATGTCTTCCAGCTGGGCATACCAGGCGcc                                                                                                                       >  6:313831/1‑149 (MQ=255)
                               tCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGAAGACATCTACCAGcagcgct                                                                                                                                  <  4:351781/137‑7 (MQ=39)
                               tCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGAAGACATCTACCAGcagcgct                                                                                                                                  >  3:351781/1‑131 (MQ=39)
                                 aGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGAc                                                                                                                    >  7:35678/1‑149 (MQ=255)
                                  gTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTTCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGAcc                                                                                                                   <  4:209803/149‑1 (MQ=255)
                                   ttCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGAt                                                                                                                                                  <  6:33604/117‑1 (MQ=255)
                                   ttCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGAt                                                                                                                                                  >  5:33604/1‑117 (MQ=255)
                                     cGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCt                                                                                                                                              <  3:30506/119‑1 (MQ=255)
                                     cGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCt                                                                                                                                              >  4:30506/1‑119 (MQ=255)
                                     cGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATCCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCCGGCGCCGACCAgg                                                                                                                >  6:384982/1‑149 (MQ=255)
                                      gcctgcctTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAgg                                                                                                                <  5:282816/148‑1 (MQ=255)
                                        ctgcctTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGAt                                                                                                                                                  <  6:309388/112‑1 (MQ=255)
                                        ctgcctTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGAt                                                                                                                                                  >  5:309388/1‑112 (MQ=255)
                                              tGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTcc                                                                                                       >  4:360964/1‑149 (MQ=255)
                                                    cACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGtt                                                                                                  >  8:14722/1‑148 (MQ=255)
                                                     aCCGTCGTCCTTGCCCCAGAAGTAGCCCATGTTGGCGCCCAGTACCCAGTTTCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGa                                                                                                <  4:37995/149‑1 (MQ=255)
                                                           gtcCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCCGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCgg                                                                                           <  6:56710/148‑1 (MQ=255)
                                                                   cccAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTcgccg                                                                                  >  5:44767/1‑149 (MQ=255)
                                                                    ccAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTcgccgc                                                                                 >  7:362635/1‑149 (MQ=255)
                                                                    ccAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTcgccgc                                                                                 >  7:272076/1‑149 (MQ=255)
                                                                    ccAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACATGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTcgccgc                                                                                 >  5:200490/1‑149 (MQ=255)
                                                                      aGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGccgccg                                                                               <  1:108046/149‑1 (MQ=255)
                                                                         aGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGc                                                                             >  1:204698/1‑148 (MQ=255)
                                                                         aGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCg                                                                            <  8:35678/149‑1 (MQ=255)
                                                                            aGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑g                                                                        >  4:301212/1‑149 (MQ=255)
                                                                                  ggTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGaaa                                                                  <  6:200490/149‑1 (MQ=255)
                                                                                       gcgcCCAGTACCCAGTTTCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGTTc                                                             <  6:217565/149‑1 (MQ=255)
                                                                                        cgcCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTcc                                                            >  8:82594/1‑149 (MQ=255)
                                                                                            cAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAgg                                                        >  2:300672/1‑149 (MQ=255)
                                                                                              gTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTg                                                      <  8:32968/149‑1 (MQ=255)
                                                                                                  ccAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAgg                                                  >  8:173372/1‑149 (MQ=255)
                                                                                                      ttGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCtgt                                              <  8:272076/149‑1 (MQ=255)
                                                                                                        gCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGCTCCGAGGTGAAGGCTGTgc                                            >  8:271650/1‑149 (MQ=255)
                                                                                                         ccc‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAg                                                         <  1:69511/135‑1 (MQ=255)
                                                                                                         ccc‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAg                                                         >  2:69511/1‑135 (MQ=255)
                                                                                                          cc‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTgcgc                                          <  1:236968/149‑1 (MQ=255)
                                                                                                           c‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGcgcg                                         <  7:173372/149‑1 (MQ=255)
                                                                                                             aGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGt                                        >  2:211884/1‑149 (MQ=255)
                                                                                                                       gATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCt                               >  4:276738/1‑148 (MQ=255)
                                                                                                                         tgctgcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCAt                            >  3:82567/1‑149 (MQ=255)
                                                                                                                          gctgcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTcc                                                            <  4:111059/116‑1 (MQ=255)
                                                                                                                          gctgcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTcc                                                            >  3:111059/1‑116 (MQ=255)
                                                                                                                          gctgcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATg                           >  2:204346/1‑149 (MQ=255)
                                                                                                                            tgcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTc                         >  4:336996/1‑149 (MQ=255)
                                                                                                                             gcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGTTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTcc                        >  1:265645/1‑149 (MQ=255)
                                                                                                                             gcACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTcc                        >  6:172493/1‑149 (MQ=255)
                                                                                                                              cACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCt                       <  2:204698/149‑1 (MQ=255)
                                                                                                                               aCCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTg                      <  2:222992/149‑1 (MQ=255)
                                                                                                                                 cTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCa                    >  3:9118/1‑149 (MQ=255)
                                                                                                                                  tGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCAt                   >  5:277180/1‑149 (MQ=255)
                                                                                                                                   ggAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATg                  >  3:6399/1‑149 (MQ=255)
                                                                                                                                     aaGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCt                <  3:276738/149‑1 (MQ=255)
                                                                                                                                       gTAGCGCGGCTGGTAGTTGTCTTCCTGGTGGGCATACCAGGGGCCGACCAGGGTGGGCTCCTGGTTGAAGGGGTATTCGCCGCCGGCGAATCTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTg               <  1:300672/149‑1 (MQ=255)
                                                                                                                                        tAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGc             <  7:14722/149‑1 (MQ=255)
                                                                                                                                        tAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGc             <  4:6399/149‑1 (MQ=255)
                                                                                                                                           cgcTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGcgtc          <  6:44767/149‑1 (MQ=255)
                                                                                                                                            gctgctGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGcgtcg         >  6:87729/1‑149 (MQ=255)
                                                                                                                                             ctgctgGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGcgtcgt        <  3:360964/149‑1 (MQ=255)
                                                                                                                                             cagctgGAAGACATCTACCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGcgtcgt        >  3:208543/3‑149 (MQ=255)
                                                                                                                                                  ggTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGCGTCGTTgcgc   >  3:314846/1‑149 (MQ=255)
                                                                                                                                                   gTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGCGTCGTTGcgcg  <  2:265645/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGAGAACAGGCCCGAAGCGGTGCGGTTGTTCAGTTCGCCTGCCTTGGCGCCACCGTCGTCCTTGCCCCAGAAGTAGCCCAGGTTGGCGCCCAGTACCCAGTTGCCC‑AGCGGCTGTTGATGCTGCACCTGGAAGTAGCGCTGCTGGTAGATGTCTTCCAGCTGGGCATACCAGGCGCCGACCAGGGTGCGCTCCTGGTTGAAGCGGTATTCGCCGCCGGCGAA‑GTTGAAACGGTCCGAGGTGAAGGCTGTGCGCGTGAACAGGCTCATGTCCTGCATGCTGGCGTCGTTGCGCG  >  NC_002947/2864553‑2864847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑