Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,868,876 G→C 11.7% intergenic (+415/‑516) PP_2520 → / → PP_2522 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,868,8760GC11.7% 153.2 / 13.0 60intergenic (+415/‑516)PP_2520/PP_2522hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base C (4/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

AAGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCG  >  NC_002947/2868729‑2869021
                                                                                                                                                   |                                                                                                                                                 
aaGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCgg                                                                                                                                                  <  4:359236/149‑1 (MQ=255)
   gtgtTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGc                                                                                                                                               <  3:280127/149‑1 (MQ=255)
         aGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTgg                                                                                                                                          <  7:49079/148‑1 (MQ=255)
           ccTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCCCGGCATTgat                                                                                                                                         >  2:382824/1‑145 (MQ=255)
           ccTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCCCGGCATTgat                                                                                                                                         <  1:382824/147‑3 (MQ=255)
               gTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCt                                                                                                                                    <  1:23168/148‑1 (MQ=255)
               gTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATtgatgaacg                                                                                                                                    <  8:128537/148‑4 (MQ=255)
                        attggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaa                                                                                                                          <  3:310935/149‑1 (MQ=255)
                         ttggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaat                                                                                                                         <  3:47308/149‑1 (MQ=255)
                            gccattCGGGTGAAGCCGTTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAg                                                                                                                      >  8:253493/1‑149 (MQ=255)
                            gccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAg                                                                                                                      >  3:49109/1‑149 (MQ=255)
                             ccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGGATTGGTGATCTGCGATATCAATCAGt                                                                                                                     <  1:11093/149‑1 (MQ=255)
                             ccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGt                                                                                                                     >  3:134628/1‑149 (MQ=255)
                               attCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTcg                                                                                                                   >  1:279922/1‑149 (MQ=255)
                                     ttGAAGCCATTTGGGAGCGGCTGGGGCATGATGCTTCGGGGCAGCCGTTCAACTCGGTTGTGCAGCTGGAGTTCTAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCttg                                                                                                             <  5:156193/148‑2 (MQ=255)
                                        aaGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTcgcg                                                                                                          >  7:361554/1‑149 (MQ=255)
                                        aaGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTcgcg                                                                                                          >  7:273297/1‑149 (MQ=255)
                                           ccATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGcc                                                                                                       >  4:282636/1‑149 (MQ=255)
                                                   gAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGtt                                                                                               <  8:14795/149‑1 (MQ=255)
                                                   gAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGtt                                                                                               >  6:308793/1‑149 (MQ=255)
                                                   gAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGATCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCAGCGATATCAATCAGTCGCGCTTCGCGCCCCCAACGtt                                                                                               >  8:173780/1‑149 (MQ=255)
                                                             ggCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATgcgc                                                                                     <  8:273297/149‑1 (MQ=255)
                                                              gCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATgcgc                                                                                     >  4:216667/1‑148 (MQ=255)
                                                               cATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGa                                                                                   >  4:224962/1‑149 (MQ=255)
                                                                           gggCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACg                                                                       <  2:279922/149‑1 (MQ=255)
                                                                                 ccGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATGAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGCCGGCttt                                                                  >  2:135130/1‑148 (MQ=255)
                                                                                 ccGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCttt                                                                  >  7:234753/1‑148 (MQ=255)
                                                                                 ccGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCttt                                                                  >  3:255633/1‑148 (MQ=255)
                                                                                 ccGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTc                                                                 <  8:361554/149‑1 (MQ=255)
                                                                                  cGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCa                                                                <  2:115555/149‑1 (MQ=255)
                                                                                   gTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGTTTTTGTCCGACGGCTTTCAg                                                               <  4:133419/149‑1 (MQ=255)
                                                                                   gTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAg                                                               <  5:308793/149‑1 (MQ=255)
                                                                                   gTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCAGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAg                                                               >  4:271400/1‑149 (MQ=255)
                                                                                       aaCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCat                                                           >  2:168634/1‑148 (MQ=255)
                                                                                        aCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAt                                                                                  <  6:338574/125‑1 (MQ=255)
                                                                                        aCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAt                                                                                  >  5:338574/1‑125 (MQ=255)
                                                                                            gTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGGGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCt                                                       >  1:320782/1‑148 (MQ=255)
                                                                                            gTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAAccc                                                        <  7:253493/147‑1 (MQ=255)
                                                                                             ttGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTca                                                     <  4:255633/149‑1 (MQ=255)
                                                                                                     gCTGGAGTTCGAGCGCGTGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTTGTGATCTGCGATATCAATCAGTCGCGCTTCGCGTCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                             <  7:46421/149‑1 (MQ=255)
                                                                                                     gCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                             <  3:282636/149‑1 (MQ=255)
                                                                                                     gCTGGAGTTCGAGCGCAGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                             <  3:271400/149‑1 (MQ=255)
                                                                                                      cTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGAt                                            <  4:49109/149‑1 (MQ=255)
                                                                                                         gAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGAt                                            <  2:320782/146‑1 (MQ=255)
                                                                                                         gAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATc                                           <  3:224962/147‑1 (MQ=255)
                                                                                                            ttCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATAc                                      >  5:221109/1‑149 (MQ=255)
                                                                                                               gAGCGCGGGCGGCCGCGTACCCCGTTCATCAATGCCGGGGCATTGGTGATCGGCGCTATCAATCAGTCGCGCTTCGCGGCCCCCCCGTTGGCGATGCGCGATTGTGGCCGACGGCGTTCCTGCACGCATGACAGCTCGAGCAGTACCCg                                   >  6:285671/1‑149 (MQ=255)
                                                                                                                     cggcggcCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCCATATCAATCAGTCGGGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGc                             <  7:173780/148‑1 (MQ=255)
                                                                                                                         ggcCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGAt                         >  2:136010/1‑149 (MQ=255)
                                                                                                                           ccGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTc                       >  2:150849/1‑149 (MQ=255)
                                                                                                                             gcgTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCgg                     <  4:134628/149‑1 (MQ=255)
                                                                                                                                 aaCCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTAAGGCAACCCTCACATCTAGATCAAAACCCGGGTCGCTGATTCGGAat                  >  7:54796/1‑148 (MQ=255)
                                                                                                                                  aCCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCg                      >  8:342723/1‑143 (MQ=255)
                                                                                                                                  aCCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCg                      <  7:342723/143‑1 (MQ=255)
                                                                                                                                    ccGTTCATCAATGCCGGGGCATAGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCa              <  5:179416/149‑1 (MQ=255)
                                                                                                                                         caccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTc         >  8:202457/4‑149 (MQ=255)
                                                                                                                                           ccaATGCCCCGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGttt          >  4:179951/2‑146 (MQ=255)
                                                                                                                                           ccaATGCCCCGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGttt          <  3:179951/145‑1 (MQ=255)
                                                                                                                                           ccaATGCCCCGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATATCGATCAAAACCCGGGTCGCAGATTCGGAATATCAGTTTcgc       >  2:282740/2‑149 (MQ=255)
                                                                                                                                             aaTGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgcgc     >  2:280381/1‑149 (MQ=255)
                                                                                                                                                 ccGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgcgcgcg  >  3:159652/1‑148 (MQ=255)
                                                                                                                                                   ggggCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACgg                                                                      <  1:282338/78‑1 (MQ=255)
                                                                                                                                                   ggggCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACgg                                                                      >  2:282338/1‑78 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCG  >  NC_002947/2868729‑2869021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑