Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,185 C→T 7.1% T897T (ACC→ACT)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,1850CT 7.1% 486.6 / 16.1 168T897T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (81/75);  new base T (8/4);  total (89/79)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

GCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCG  >  NC_002947/197037‑197333
                                                                                                                                                      |                                                                                                                                                       
gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAcc                                                                                                                                                         >  3:242308/1‑149 (MQ=38)
gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAcc                                                                                                                                                         <  7:256641/149‑1 (MQ=38)
       cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCg                                                                                                                                                  <  1:100508/149‑1 (MQ=17)
       cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCAAGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGCCCGCGTAATCACCTacacag                                                                                                                                                  >  6:259705/1‑147 (MQ=255)
       cATCGAGAACGCCACAGGCGGTAATTTCGAGAAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAAAGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCAGTCGGTCACCGAAGGCGGCGTAATCACCTACACCg                                                                                                                                                  >  5:144732/1‑149 (MQ=255)
        aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGt                                                                                                                                                 >  6:344912/1‑149 (MQ=37)
          cGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGa                                                                                                                                               >  4:219477/1‑149 (MQ=37)
           gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAc                                                                                                                                              >  4:210598/1‑149 (MQ=17)
           gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCTTGa                                                                                                                                               <  2:383250/148‑1 (MQ=37)
             gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAc‑cc                                                                                                                                           <  1:171159/149‑1 (MQ=37)
             gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAc‑cc                                                                                                                                           >  1:263852/1‑149 (MQ=37)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  5:106758/149‑1 (MQ=37)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  7:67962/149‑1 (MQ=37)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  1:351630/149‑1 (MQ=37)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  3:219477/149‑1 (MQ=37)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCAAGCCCGTCTGTCACAGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  7:127941/149‑1 (MQ=255)
                cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        <  6:69140/149‑1 (MQ=37)
                 gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAg                                                                                                                                       <  3:19713/149‑1 (MQ=17)
                 gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAg                                                                                                                                       <  6:134499/149‑1 (MQ=37)
                  ccACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCGCCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAg                                                                                                                                       >  3:223112/1‑148 (MQ=12)
                   cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGc                                                                                                                                      >  2:319451/1‑148 (MQ=17)
                   cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGCTCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATAACCTACACCGTGAC‑CCTGAGc                                                                                                                                      >  5:213484/1‑148 (MQ=255)
                      aGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATc                                                                                                                                  >  3:245828/1‑149 (MQ=35)
                      aGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATc                                                                                                                                  >  4:59154/1‑149 (MQ=35)
                       ggCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATcc                                                                                                                                 <  2:263852/149‑1 (MQ=34)
                        gCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCt                                                                                                                                >  7:152498/1‑149 (MQ=34)
                        gCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCt                                                                                                                                >  7:79203/1‑149 (MQ=34)
                        gCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCTATCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCt                                                                                                                                <  1:300250/149‑1 (MQ=255)
                               tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGa                                                                                                                         >  4:292318/1‑149 (MQ=34)
                                 tCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGAc                                                                                                                        <  7:363942/148‑1 (MQ=34)
                                   gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGcc                                                                                                                     >  1:68258/1‑149 (MQ=34)
                                   gAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGcc                                                                                                                     >  8:356778/1‑149 (MQ=21)
                                    aGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCg                                                                                                                    <  3:315649/149‑1 (MQ=34)
                                    aGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCg                                                                                                                    <  7:356778/149‑1 (MQ=21)
                                       aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGtg                                                                                                                 <  3:84981/149‑2 (MQ=18)
                                        aCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTaa                                                                                                                >  5:52238/1‑149 (MQ=35)
                                        aCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTAAACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTaa                                                                                                                >  7:51103/1‑149 (MQ=25)
                                         cTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGtga                                                                                                                >  6:104186/1‑146 (MQ=17)
                                             ccccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACAGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTg                                                                                                           >  6:368112/1‑149 (MQ=255)
                                              cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGa                                                                                                          <  7:127751/149‑1 (MQ=39)
                                              cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGa                                                                                                          >  8:37287/1‑149 (MQ=39)
                                                cGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAcc                                                                                                        >  4:23131/1‑149 (MQ=39)
                                                       gACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATc                                                                                                                                  <  3:169777/116‑1 (MQ=35)
                                                       gACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATc                                                                                                                                  >  4:169777/1‑116 (MQ=35)
                                                              ggtCATACCACGTTCAACGACTCGGTGG‑ATGCCACCACCGCCTCAGCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGcc                                                                                           <  1:324973/147‑1 (MQ=11)
                                                               cTCAGACCACGATCAACGACTCGGTCGAATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca                                                                                                                                                     >  1:17717/1‑91 (MQ=16)
                                                                  aGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGt                                                                                       >  1:167850/1‑148 (MQ=37)
                                                                    aCCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGa                                                                                                                                        >  4:317267/1‑97 (MQ=33)
                                                                    aCCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtcat                                                                                    >  1:108641/1‑149 (MQ=39)
                                                                    aCCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtca                                                                                     >  5:245945/1‑148 (MQ=38)
                                                                      cACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtcatc                                                                                   >  2:99853/1‑148 (MQ=40)
                                                                             aaCGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGa                                                                           <  8:14741/149‑1 (MQ=255)
                                                                              aCGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGa‑‑a                                                                        <  2:167850/149‑1 (MQ=255)
                                                                              aCGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGa‑‑a                                                                        <  5:344912/149‑1 (MQ=255)
                                                                                  cTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCg                                                                    >  6:207673/1‑149 (MQ=255)
                                                                                  cTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCg                                                                    >  4:305917/1‑149 (MQ=255)
                                                                                   tCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCgg                                                                   >  3:335542/1‑149 (MQ=255)
                                                                                       tCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGc                                                                  <  8:198935/146‑1 (MQ=255)
                                                                                       tCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGc                                                                  >  7:198935/1‑146 (MQ=255)
                                                                                           aTGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg                                                                                              <  1:140149/117‑1 (MQ=35)
                                                                                           aTGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg                                                                                              >  2:140149/1‑117 (MQ=35)
                                                                                              ccaccaCCGCGCC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAg                                                         >  3:26514/1‑149 (MQ=255)
                                                                                              ccaccaCCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCa                                                          <  6:52238/148‑1 (MQ=255)
                                                                                              ccaccaCCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCa                                                          <  3:59154/148‑1 (MQ=255)
                                                                                               caccacCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAgg                                                        >  2:255656/1‑149 (MQ=255)
                                                                                               caccacCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGAGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAg                                                         <  4:245828/148‑1 (MQ=255)
                                                                                               caccacCGCGAC‑CCTGACGGCGAGCCCGTCGGACACCGAAGGCGGCGTAATCACCTACACCGAGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTCTCCAACGTCGAGGTCATCACAGTAGA‑‑AGCCGCCAAAACCCAgg                                                        >  8:274923/1‑149 (MQ=14)
                                                                                                accaccGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAggg                                                       >  6:211231/1‑149 (MQ=255)
                                                                                                accaccGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAggg                                                       <  8:51103/149‑1 (MQ=255)
                                                                                                  caccGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAggg                                                       >  1:338604/1‑147 (MQ=255)
                                                                                                    ccGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGc                                                   >  6:286147/1‑149 (MQ=255)
                                                                                                          c‑ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAt                                             >  2:256354/1‑149 (MQ=255)
                                                                                                            ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAtt                                            <  3:358798/149‑1 (MQ=255)
                                                                                                            ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAtt                                            >  8:123146/1‑149 (MQ=255)
                                                                                                            ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAtt                                            >  2:228118/1‑149 (MQ=255)
                                                                                                             cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAttt                                           <  6:245945/149‑1 (MQ=255)
                                                                                                             cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAttt                                           <  2:113587/149‑1 (MQ=255)
                                                                                                              tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACGCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGAttt                                           >  1:295797/1‑149 (MQ=255)
                                                                                                              tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTc                                          >  8:84060/1‑149 (MQ=255)
                                                                                                                aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                        <  2:330157/149‑1 (MQ=255)
                                                                                                                       gCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCg                                 >  2:98611/1‑149 (MQ=255)
                                                                                                                         ccGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGc                               <  5:207673/149‑1 (MQ=255)
                                                                                                                              ggTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATg                          <  7:97017/149‑1 (MQ=255)
                                                                                                                               gTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGa                         >  2:357336/1‑149 (MQ=255)
                                                                                                                                tCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGAc                        <  1:99853/149‑1 (MQ=40)
                                                                                                                                  aCCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGTCAGCGTCGATTTCCAGACCCCGGCCAATGACGt                      <  5:259705/149‑1 (MQ=255)
                                                                                                                                   ccGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAcc                                                                                                        >  7:43230/1‑68 (MQ=35)
                                                                                                                                   ccGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTc                     >  6:34354/1‑149 (MQ=39)
                                                                                                                                      aaGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                  <  5:286147/149‑1 (MQ=39)
                                                                                                                                      aaGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTa                   >  5:89171/1‑148 (MQ=39)
                                                                                                                                       aGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                  >  5:134921/1‑148 (MQ=39)
                                                                                                                                                 aTCACCTACACCGTGAC‑CCTGAGCATTCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTc       <  7:274923/149‑1 (MQ=21)
                                                                                                                                                  tCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTc                                                                                                   >  2:243406/1‑58 (MQ=255)
                                                                                                                                                  tCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTc                                                                                                   <  1:243406/58‑1 (MQ=255)
                                                                                                                                                  tCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCaaaa                                                              >  2:174159/1‑93 (MQ=37)
                                                                                                                                                  tCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAACACCCAGGGCCGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACACCAACGGATc       >  4:14842/1‑148 (MQ=255)
                                                                                                                                                  tCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTc       >  8:68930/1‑148 (MQ=34)
                                                                                                                                                  tCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCg      <  3:169188/149‑1 (MQ=34)
                                                                                                                                                    aCCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTCAATAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCg      <  1:121246/149‑1 (MQ=255)
                                                                                                                                                      cTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCg  >  7:25791/1‑149 (MQ=34)
                                                                                                                                                      |                                                                                                                                                       
GCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCG‑ATGCCACCACCGCGAC‑CCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGA‑‑AGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCG  >  NC_002947/197037‑197333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑