Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,969,556	A→C	6.8%	I73L (ATC→CTC)	PP_2598 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,969,556	0	A	C	6.8%	215.4 / 4.8	69	I73L (ATC→CTC)	PP_2598	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/29);  new base C (3/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGCTACGATCCAC  >  NC_002947/2969408‑2969704                                                                                                                                                      |                                                                                                                                                       ggCGCTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGa                                                                                                                                                        >  1:23790/1‑149 (MQ=255)  gcgcTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGa                                                                                                                                                        <  5:214468/148‑1 (MQ=255)  gcgcTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGa                                                                                                                                                        <  5:149661/148‑1 (MQ=255)     cTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGa                                                                                                                                                    <  3:240804/149‑1 (MQ=255)          cgtcgtGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAACCCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCgg                                                                                                                                               >  7:228279/1‑149 (MQ=255)                gCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCaaa                                                                                                                                         <  8:232819/149‑1 (MQ=255)                  tGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATTGCGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAag                                                                                                                                        >  1:102000/1‑149 (MQ=255)                    ggCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAagca                                                                                                                                      <  2:173997/148‑1 (MQ=255)                             ctgctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCct                                                                                                                            >  4:340109/1‑149 (MQ=255)                             ctgctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCct                                                                                                                            >  3:339873/1‑149 (MQ=255)                               gctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCctgc                                                                                                                          >  6:249941/1‑149 (MQ=255)                               gctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCGGCTCGATCAGgcgcctg                                                                                                                                         <  5:109304/134‑4 (MQ=255)                               gctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCGGCTCGATCAGgcgcctg                                                                                                                                         <  5:103942/134‑4 (MQ=255)                               gctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCGGCTCGATCAGgcgcctg                                                                                                                                         >  6:103942/1‑131 (MQ=255)                               gctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCGGCTCGATCAGgcgcctg                                                                                                                                         >  6:109304/1‑131 (MQ=255)                                ctCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCctgc                                                                                                                          <  8:169471/148‑1 (MQ=255)                                 tCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCctgctg                                                                                                                        >  3:366610/1‑149 (MQ=255)                                     cgccgcCCTGGCCTGGCCGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTc                                                                                                                    <  4:96904/149‑1 (MQ=255)                                      gccgccCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGCCGCGCGGGCCCGGCGCCTGATCGCGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTca                                                                                                                   >  3:65698/1‑149 (MQ=255)                                        cgccCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTcaca                                                                                                                 <  4:366610/149‑1 (MQ=255)                                         gccCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCacaa                                                                                                                >  2:196509/1‑149 (MQ=255)                                          cccTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCacaa                                                                                                                >  3:222362/1‑148 (MQ=255)                                          cccTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCacaa                                                                                                                >  8:20532/1‑148 (MQ=255)                                            ctggcctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAacac                                                                                                             <  6:202988/149‑1 (MQ=255)                                            ctggcctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAacac                                                                                                             >  2:180726/1‑149 (MQ=255)                                               gcctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCGGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACacgac                                                                                                          >  6:235907/1‑149 (MQ=255)                                               gcctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACacgac                                                                                                          <  8:332650/149‑1 (MQ=255)                                               gcctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGACGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACacga                                                                                                           <  8:365079/148‑1 (MQ=255)                                                 ctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCacaac                                                                                                               <  6:220003/142‑1 (MQ=255)                                                 ctggcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCacaac                                                                                                               >  5:220003/1‑142 (MQ=255)                                                    gcAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTg                                                                                                      <  5:280067/148‑1 (MQ=255)                                                             ccGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑gg                                                                                          <  1:196509/149‑1 (MQ=255)                                                             ccGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑gg                                                                                          <  3:340109/149‑1 (MQ=255)                                                             ccGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑gg                                                                                          <  4:222362/149‑1 (MQ=255)                                                                cgGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGca                                                                                        >  3:193319/1‑148 (MQ=255)                                                                     tt‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAgcgc                                                                                  <  8:228279/149‑1 (MQ=255)                                                                         gCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCt                                                                                <  1:180726/148‑1 (MQ=255)                                                                         gCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTg                                                                               >  2:236098/1‑149 (MQ=255)                                                                           cAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGa                                                                             <  5:293681/149‑1 (MQ=255)                                                                            aaaCCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGAt                                                                            >  3:7699/1‑149 (MQ=255)                                                                                ccTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGc                                                                        >  1:30567/1‑149 (MQ=255)                                                                                  tCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTg                                                                      >  4:13529/1‑149 (MQ=255)                                                                                  tCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTg                                                                      >  5:123019/1‑149 (MQ=255)                                                                                    gTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGaagatcgg                                                                      <  4:193319/147‑8 (MQ=255)                                                                                                 gCGATGCCGCCCAGCGGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCg                                                       <  5:235907/149‑1 (MQ=255)                                                                                                 gCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCtttt                                                              >  1:153727/1‑142 (MQ=255)                                                                                                 gCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATc                                                        <  2:30567/148‑1 (MQ=255)                                                                                                 gCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCACTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCtttt                                                              >  2:88430/1‑142 (MQ=255)                                                                                                   gATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGc                                                                                                                              >  1:324493/1‑78 (MQ=255)                                                                                                   gATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGc                                                                                                                              <  2:324493/78‑1 (MQ=255)                                                                                                       ccgccCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGgcgc                                                  <  2:23790/148‑1 (MQ=255)                                                                                                       ccgccCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCt                                                 >  4:70141/1‑149 (MQ=255)                                                                                                        cgccCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCt                                                 >  3:269638/1‑148 (MQ=255)                                                                                                         gccCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTg                                                >  6:111295/1‑148 (MQ=255)                                                                                                               cagGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACttgt                                         <  4:269638/149‑1 (MQ=255)                                                                                                                agGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACttgtt                                        <  2:102000/149‑1 (MQ=255)                                                                                                                  gTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACttgttg                                       >  3:189232/1‑148 (MQ=255)                                                                                                                      ccAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGcaggc                                  >  7:213920/1‑149 (MQ=255)                                                                                                                       cAAGACGATCGACGAGAGGGCCAGGCTCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGTCTCATAACACGACGGTGCACACCCACTGGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGcagg                                   <  1:106809/149‑1 (MQ=255)                                                                                                                         acaCGATCGACCAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGATGGCTCACAACACGACGGTGAACACCCA‑‑GGCACAGCGCCTGGATCGGATGGCGCTTTTAAGATCGGGAGCTGAACTTGTTGcatgccca                               >  8:315813/3‑149 (MQ=255)                                                                                                                         aGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGcaggcc                                 >  4:182392/1‑147 (MQ=255)                                                                                                                          gACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGcaggcccag                              <  3:182392/149‑1 (MQ=255)                                                                                                                               tCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACttgt                                         <  4:125518/133‑1 (MQ=255)                                                                                                                               tCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACttgt                                         >  3:125518/1‑133 (MQ=255)                                                                                                                                  acgaGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAAcc                      >  5:132835/1‑149 (MQ=255)                                                                                                                                         ggCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTAt               >  3:294354/1‑149 (MQ=255)                                                                                                                                          gctttgcgcCGGCTCGATCAGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCATACCTGACTATc              >  6:183832/6‑149 (MQ=255)                                                                                                                                          gCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATc              <  1:154843/149‑1 (MQ=255)                                                                                                                                            cAGGCGCCTTATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGGGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGc            <  8:261462/149‑1 (MQ=255)                                                                                                                                            cAGCCGCCTGATCGCGCCGTCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGCTCGGCTGGCGCTTTTAAGATCGGGCGCTGACCTTGTTCCAGGCCCAGGCCAATCCTGACTATCGc            >  8:68878/1‑149 (MQ=255)                                                                                                                                               gcgcCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGCTAc         >  7:17269/1‑149 (MQ=255)                                                                                                                                                    tGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGCTACGATc     >  5:191365/1‑148 (MQ=255)                                                                                                                                                      aTCGAGCCGGCGCAAAGCAGCACTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGCTACGATCCAc  <  1:88430/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GGCGCTGGCCGTCGTGCTGGGCATGCTGCTGCTCGGCGCCGCCCTGGCCTGGCAGGGTTACCGCGGAATT‑CGCCAAACCCTCGTGGCCGCCGCTGGCGATGCCGCCCAGCAGGTTGCCAAGACGATCGACGAGCGGGCCAGGCGCCTGATCGAGCCGGCGCAAAGCAGCATTCGCCTGCTGGCTCACAACACGACGGTGCACACCCA‑‑GGCACAGCGCCTGGATCGGCTGGCGCTTTTAAGATCGGGCGCTGAACTTGTTGCAGGCCCAGGCCAAACCTGACTATCGCTACGATCCAC  >  NC_002947/2969408‑2969704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑