Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,972,643 G→C 11.3% L411V (CTG→GTG)  PP_2600 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,972,6430GC11.3% 155.2 / 10.2 53L411V (CTG→GTG) PP_2600hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (4/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CGGGTCCTGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGAAA  >  NC_002947/2972497‑2972788
                                                                                                                                                  |                                                                                                                                                 
cGGGTCCTGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCg                                                                                                                                                 >  1:335531/1‑149 (MQ=255)
       tGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCGCCCGGCACCGCTGCcgg                                                                                                                                          >  7:270146/1‑149 (MQ=255)
         gCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCcgg                                                                                                                                          <  8:26092/147‑1 (MQ=255)
           cGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCACCGCTGCcggtagg                                                                                                                                      >  6:77719/1‑145 (MQ=255)
            gTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCcgggcggg                                                                                                                                     >  3:64660/1‑149 (MQ=255)
            gTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCGCCCGGCACCGCTGCcggtaggg                                                                                                                                     <  8:270146/149‑6 (MQ=255)
               aCGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCa                                                                                                                                   <  3:356133/148‑1 (MQ=255)
               aCGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCACCGCTGCCggtagggcc                                                                                                                                   <  5:77719/148‑2 (MQ=255)
                cGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCAt                                                                                                                                  <  4:64660/148‑1 (MQ=255)
                 gggCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGc                                                                                                                                >  3:235201/1‑149 (MQ=255)
                       gggTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAgg                                                                                                                          >  3:353062/1‑149 (MQ=255)
                            ttGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCAt                                                                                                                      <  1:362663/148‑1 (MQ=255)
                               aaGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcg                                                                                                                  >  8:9157/1‑149 (MQ=255)
                                aGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcgg                                                                                                                 >  3:139646/1‑149 (MQ=255)
                                aGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcgg                                                                                                                 >  4:62992/1‑149 (MQ=255)
                                      tGACCGTGGCGCGCAGCCCGGTCGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCaag                                                                                                           <  4:101971/149‑1 (MQ=255)
                                       gACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCAAACCGGCGCacg                                                                                                           >  7:242860/1‑146 (MQ=255)
                                         ccGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCaagaag                                                                                                        >  2:180012/1‑149 (MQ=255)
                                            tGGCGCGCAGCCCGGACGGGTCAATGCCGCGGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCga                                                                                                     <  5:378253/149‑1 (MQ=255)
                                             ggCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCgat                                                                                                    <  8:211967/149‑1 (MQ=255)
                                                gcgcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCgatgat                                                                                                 >  2:24223/1‑149 (MQ=255)
                                                 cgcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATc                                                                                                <  2:335531/149‑1 (MQ=255)
                                                  gcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCg                                                                                               <  2:148583/149‑1 (MQ=255)
                                                      cccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCaaa                                                                                           >  8:257370/1‑149 (MQ=255)
                                                       ccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAAc                                                                                          <  6:157529/149‑1 (MQ=255)
                                                       ccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAAc                                                                                          <  4:369493/149‑1 (MQ=255)
                                                               gTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCAc                                                                                   >  6:47211/1‑148 (MQ=255)
                                                                  aaTGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAg                                                                                >  5:290786/1‑148 (MQ=255)
                                                                  aaTGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGc                                                                               <  7:9157/149‑1 (MQ=255)
                                                                         cAGGCCTTGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACAcctc                                                                        <  7:130264/149‑1 (MQ=255)
                                                                                 ggCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGcc                                                                  <  1:180012/147‑1 (MQ=255)
                                                                                  gCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTg                                                               >  5:11573/1‑149 (MQ=255)
                                                                                   cGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGc                                                              <  6:290786/149‑1 (MQ=255)
                                                                                       ctgctCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCa                                                          >  7:9572/1‑149 (MQ=255)
                                                                                          ctCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACt                                                        <  8:9572/148‑1 (MQ=255)
                                                                                             gAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGc                                                      <  6:11573/147‑1 (MQ=255)
                                                                                             gAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAg                                                    >  1:210302/1‑149 (MQ=255)
                                                                                                cGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGa                                                 <  5:47211/149‑1 (MQ=255)
                                                                                                     cgccgcGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATg                                            <  1:24223/149‑1 (MQ=255)
                                                                                                        cgcgTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATTGcc                                         >  8:141188/1‑149 (MQ=255)
                                                                                                          cgTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGa                                       <  3:62992/149‑1 (MQ=255)
                                                                                                                aGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATa                                 <  7:277551/149‑1 (MQ=255)
                                                                                                                   tAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTc                              <  4:153187/149‑1 (MQ=255)
                                                                                                                    aGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCg                             >  4:179990/1‑149 (MQ=255)
                                                                                                                      ccGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGt                            >  4:164265/1‑148 (MQ=255)
                                                                                                                             gcACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCCCACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCa                    >  7:176352/1‑149 (MQ=255)
                                                                                                                             gcACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCa                    >  3:303431/1‑149 (MQ=255)
                                                                                                                               acaTGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATg                  >  2:385450/1‑149 (MQ=255)
                                                                                                                                     ggCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTc            <  7:257370/149‑1 (MQ=255)
                                                                                                                                        ccgcccGGCACCGCTGCCGGTAGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  6:390283/5‑149 (MQ=255)
                                                                                                                                        ccgcccGGCACCGCTGCCGGTAGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  8:143683/5‑149 (MQ=255)
                                                                                                                                         cTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  1:109049/1‑148 (MQ=255)
                                                                                                                                               gCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGaaa  <  4:139646/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGGTCCTGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGAAA  >  NC_002947/2972497‑2972788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑