Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,972,646 G→C 11.5% P410A (CCG→GCG)  PP_2600 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,972,6460GC11.5% 148.2 / 9.5 52P410A (CCG→GCG) PP_2600hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base C (4/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

TGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGAAA  >  NC_002947/2972504‑2972788
                                                                                                                                              |                                                                                                                                              
tGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCGCCCGGCACCGCTGCcgg                                                                                                                                          >  7:270146/1‑149 (MQ=255)
  gCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCcgg                                                                                                                                          <  8:26092/147‑1 (MQ=255)
    cGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCACCGCTGCcggtagg                                                                                                                                      >  6:77719/1‑145 (MQ=255)
     gTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCcgggcggg                                                                                                                                     >  3:64660/1‑149 (MQ=255)
     gTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCGCCCGGCACCGCTGCcggtaggg                                                                                                                                     <  8:270146/149‑6 (MQ=255)
        aCGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCa                                                                                                                                   <  3:356133/148‑1 (MQ=255)
        aCGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCACCGCTGCCggtagggcc                                                                                                                                   <  5:77719/148‑2 (MQ=255)
         cGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCAt                                                                                                                                  <  4:64660/148‑1 (MQ=255)
          gggCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGc                                                                                                                                >  3:235201/1‑149 (MQ=255)
                gggTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAgg                                                                                                                          >  3:353062/1‑149 (MQ=255)
                     ttGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCAt                                                                                                                      <  1:362663/148‑1 (MQ=255)
                        aaGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcg                                                                                                                  >  8:9157/1‑149 (MQ=255)
                         aGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcgg                                                                                                                 >  3:139646/1‑149 (MQ=255)
                         aGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAgcgg                                                                                                                 >  4:62992/1‑149 (MQ=255)
                               tGACCGTGGCGCGCAGCCCGGTCGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCaag                                                                                                           <  4:101971/149‑1 (MQ=255)
                                gACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCAAACCGGCGCacg                                                                                                           >  7:242860/1‑146 (MQ=255)
                                  ccGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCaagaag                                                                                                        >  2:180012/1‑149 (MQ=255)
                                     tGGCGCGCAGCCCGGACGGGTCAATGCCGCGGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCga                                                                                                     <  5:378253/149‑1 (MQ=255)
                                      ggCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCgat                                                                                                    <  8:211967/149‑1 (MQ=255)
                                         gcgcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCgatgat                                                                                                 >  2:24223/1‑149 (MQ=255)
                                          cgcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATc                                                                                                <  2:335531/149‑1 (MQ=255)
                                           gcAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCg                                                                                               <  2:148583/149‑1 (MQ=255)
                                               cccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCaaa                                                                                           >  8:257370/1‑149 (MQ=255)
                                                ccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAAc                                                                                          <  4:369493/149‑1 (MQ=255)
                                                ccGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAAc                                                                                          <  6:157529/149‑1 (MQ=255)
                                                        gTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCAc                                                                                   >  6:47211/1‑148 (MQ=255)
                                                           aaTGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAg                                                                                >  5:290786/1‑148 (MQ=255)
                                                           aaTGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGc                                                                               <  7:9157/149‑1 (MQ=255)
                                                                  cAGGCCTTGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACAcctc                                                                        <  7:130264/149‑1 (MQ=255)
                                                                          ggCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGcc                                                                  <  1:180012/147‑1 (MQ=255)
                                                                           gCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTg                                                               >  5:11573/1‑149 (MQ=255)
                                                                            cGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGc                                                              <  6:290786/149‑1 (MQ=255)
                                                                                ctgctCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCa                                                          >  7:9572/1‑149 (MQ=255)
                                                                                   ctCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACt                                                        <  8:9572/148‑1 (MQ=255)
                                                                                      gAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGc                                                      <  6:11573/147‑1 (MQ=255)
                                                                                      gAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAg                                                    >  1:210302/1‑149 (MQ=255)
                                                                                         cGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGa                                                 <  5:47211/149‑1 (MQ=255)
                                                                                              cgccgcGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATg                                            <  1:24223/149‑1 (MQ=255)
                                                                                                 cgcgTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATTGcc                                         >  8:141188/1‑149 (MQ=255)
                                                                                                   cgTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGa                                       <  3:62992/149‑1 (MQ=255)
                                                                                                         aGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATa                                 <  7:277551/149‑1 (MQ=255)
                                                                                                            tAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTc                              <  4:153187/149‑1 (MQ=255)
                                                                                                             aGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCg                             >  4:179990/1‑149 (MQ=255)
                                                                                                               ccGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGt                            >  4:164265/1‑148 (MQ=255)
                                                                                                                      gcACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCCCACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCa                    >  7:176352/1‑149 (MQ=255)
                                                                                                                      gcACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCa                    >  3:303431/1‑149 (MQ=255)
                                                                                                                        acaTGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATg                  >  2:385450/1‑149 (MQ=255)
                                                                                                                              ggCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTc            <  7:257370/149‑1 (MQ=255)
                                                                                                                                 ccgcccGGCACCGCTGCCGGTAGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  8:143683/5‑149 (MQ=255)
                                                                                                                                 ccgcccGGCACCGCTGCCGGTAGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  6:390283/5‑149 (MQ=255)
                                                                                                                                  cTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGtt         >  1:109049/1‑148 (MQ=255)
                                                                                                                                        gCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGaaa  <  4:139646/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
TGGCCGTTACGGGCATGGGTATTGAAGTCAGTGACCGTGGCGCGCAGCCCGGACGGGTCAATGCCGCAGGCCTGGGCGAGCTGCTCGAGCGTAGCGCCGCGTTTCAGGTAGCCGTTGCGCACATGCGGCCCTACCGGCAGCGGTGCCGGGCGGGCATGCCCCAGGCCATAGCGGCGCAAGAAGCGATGATCGCAAACCAGCCACGAGCACACCTCCTGGCCCTGCGGCACTGCAGCGACCATGGCCGACACATAGTCGTAATAGCCATGCGCTTCGTTGACGAAA  >  NC_002947/2972504‑2972788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑