Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,054,731 G→T 14.2% intergenic (‑206/+102) PP_2666 ← / ← PP_2667 hypothetical protein/ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,054,7310GT14.2% 101.4 / 8.1 41intergenic (‑206/+102)PP_2666/PP_2667hypothetical protein/ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (9/24);  new base T (3/3);  total (14/27)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCGACGATAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGT  >  NC_002947/3054583‑3054874
                                                                                                                                                     |                                                                                                                                               
gCGACGATAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCcgcg                                                                                                                                                 >  3:227727/1‑149 (MQ=255)
   acgaTAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAg                                                                                                                                              >  2:276436/1‑149 (MQ=255)
    cgaTAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGc                                                                                                                                             <  7:186293/149‑1 (MQ=255)
      aTAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGCAAGGGCCGCGCCGCgg                                                                                                                                           >  7:274306/1‑149 (MQ=255)
           cAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGGCGCGAAAAGGCCCCCCAACGGCCCCa                                                                                                                                      >  7:257733/1‑149 (MQ=37)
             gTAAATGCAGTACTAAAGTAGTAGATGGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGGGCGGCCTTGCGTGGGGAAAGGGGCCCCCAGCGGcccccg                                                                                                                                     >  1:221026/1‑147 (MQ=37)
             gTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAgg                                                                                                                                    >  7:62799/1‑149 (MQ=255)
             gTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAg                                                                                                                                     >  4:68832/1‑148 (MQ=255)
              tAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCTGCGCGGccctttcg                                                                                                                                   <  3:287122/149‑6 (MQ=255)
              tAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGa                                                                                                                                   >  3:28975/1‑149 (MQ=255)
                aaTGCAGTACTAAAGTACTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGAt                                                                                                                                  <  3:232638/148‑1 (MQ=255)
                    cAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCg                                                                                                                              <  3:55441/148‑1 (MQ=255)
                     aGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGc                                                                                                                            <  4:227727/149‑1 (MQ=255)
                            aaGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGcatcat                                                                                                                       <  4:28975/147‑1 (MQ=255)
                               tagtagAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATa                                                                                                                  <  7:229060/149‑1 (MQ=255)
                                  tagAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGAt                                                                                                               <  4:226971/149‑1 (MQ=255)
                                          ccGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTggg                                                                                                        <  1:276436/149‑1 (MQ=255)
                                           cGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTgggg                                                                                                       <  3:68832/149‑1 (MQ=255)
                                                      aGGCATGGAGACGCCCTAGGGAGCAACTGCCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCgg                                                                                            <  8:155946/149‑1 (MQ=255)
                                                          aTGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGCAAGGGCCGCGCCGCGGGCCCCGGGGCGCGGGCTGAAGCATAGAGTGCTGGGGCCGCGTTGGGGcgc                                                                                         >  3:280194/1‑146 (MQ=21)
                                                                        gggAGCAACTGTCTTGCTCAATTTCTGATAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAgg                                                                          <  8:62799/149‑1 (MQ=255)
                                                                                        cTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGt                                                          <  2:132543/149‑1 (MQ=255)
                                                                                           attttCTGAAAGCTGGCTCGTTCGCTGTGTGAGCGGCCTTGTTTCGCGAAAGGGCCGCGCTGCGGACGCTGGATCTCGTCATCATGCTTAGATTGCTGGTGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTac                                                       <  4:335200/147‑1 (MQ=25)
                                                                                                   aacgCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCa                                                 <  7:163118/144‑1 (MQ=255)
                                                                                                   aaaGCTGGCGCGATCCCTGTGGGCGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAg                                                <  2:221026/148‑1 (MQ=255)
                                                                                                     aGCTGGCGCGATGCCTGTGGGAGCGGCCTGGCGTCGCGAATGGGCCGCGCTGCGGCCCCAGGAGCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGtt                                             <  8:257733/149‑1 (MQ=37)
                                                                                                          gcgcGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTc                                          <  3:111212/147‑1 (MQ=255)
                                                                                                                   cTGGGGGAGCGGCCTTGCCTCGCGAACGGGCCGCGCAGCGGCCCCAGGATCTCGCCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTg                               <  7:179823/149‑1 (MQ=255)
                                                                                                                            cGGCATTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAgcgc                       <  4:234569/148‑1 (MQ=255)
                                                                                                                                 ttGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATg                 >  3:203900/1‑149 (MQ=255)
                                                                                                                                   gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCgg                                   <  8:148779/129‑1 (MQ=255)
                                                                                                                                   gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCgg                                   >  7:148779/1‑129 (MQ=255)
                                                                                                                                     gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgg             <  3:44584/149‑1 (MQ=255)
                                                                                                                                        gcgAAAGGGCCGCGCAGCGGCCCCAGTATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgggg           <  8:137463/148‑1 (MQ=255)
                                                                                                                                         cgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGt         <  4:280194/149‑1 (MQ=255)
                                                                                                                                           ctggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGtt        >  3:265888/4‑148 (MQ=255)
                                                                                                                                            tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCCCACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCt               >  6:14779/3‑140 (MQ=38)
                                                                                                                                            tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCt               <  5:14779/138‑1 (MQ=255)
                                                                                                                                            tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTg       >  6:105139/3‑148 (MQ=255)
                                                                                                                                              gggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGaa     <  4:265888/148‑1 (MQ=255)
                                                                                                                                                gCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCa                   <  2:265975/132‑1 (MQ=255)
                                                                                                                                                gCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCa                   >  1:265975/1‑132 (MQ=255)
                                                                                                                                                  cgcgCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGt  >  6:381758/1‑147 (MQ=255)
                                                                                                                                                  cgcgCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCAACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGa      <  6:5588/143‑1 (MQ=255)
                                                                                                                                                  cgcgCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCAACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGa      >  5:5588/1‑143 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GCGACGATAGACAGTAAATGCAGTACTAAAGTAGTAGAT‑GGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGT  >  NC_002947/3054583‑3054874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑