Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,075,135	A→C	8.5%	intergenic (+30/+195)	PP_2684 → / ← PP_2685	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,075,135	0	A	C	8.5%	167.6 / 7.8	59	intergenic (+30/+195)	PP_2684/PP_2685	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base C (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

GTGATGATCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCTGTGGGAGCGGG  >  NC_002947/3074989‑3075278                                                                                                                                                   |                                                                                                                                                  gTGATGATCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAgc                                                                                                                                                 >  7:349294/1‑149 (MQ=255) gTGATGATCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAgc                                                                                                                                                 <  5:3609/149‑1 (MQ=255)      gatCATGCTTGGCTTTGCCCTGATGGCCGCACTGACACAGTGGGCCCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCTGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAgcggcg                                                                                                                                             >  7:277185/1‑148 (MQ=255)       atCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCTTCGCGgcgc                                                                                                                                            <  5:67609/148‑1 (MQ=255)        tCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAgcg                                                                                                                                                >  6:174150/1‑143 (MQ=255)        tCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAgcg                                                                                                                                                <  5:174150/143‑1 (MQ=255)        tCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGGGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCTTCGCGgcgccg                                                                                                                                          <  7:166011/149‑3 (MQ=255)          cTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGCCGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTcgc                                                                                                                                        >  4:178433/1‑149 (MQ=255)          cTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCTTCGCGgcgccgtt                                                                                                                                        <  6:185283/149‑5 (MQ=255)                 gCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGCCGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTAc                                                                                                                                  <  3:178433/148‑1 (MQ=255)                   tttGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTAcgcg                                                                                                                               >  6:337608/1‑149 (MQ=255)                     tGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGt                                                                                                                              <  3:39306/148‑1 (MQ=255)                      gCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGttt                                                                                                                            >  1:199897/1‑149 (MQ=255)                      gCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGtt                                                                                                                             <  5:360938/148‑1 (MQ=255)                       cccTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGttt                                                                                                                            <  6:291357/148‑1 (MQ=255)                       cccTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTg                                                                                                                           >  8:218837/1‑149 (MQ=255)                        ccTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGaa                                                                                                                                                   >  8:247673/1‑124 (MQ=255)                        ccTGATGGCCGCACTGACCCAGTGCGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGaa                                                                                                                                                   <  7:247673/124‑1 (MQ=255)                           gATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTg                                                                                                                       >  3:268451/1‑149 (MQ=255)                                ccGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGc                                                                                                                  >  6:353808/1‑149 (MQ=255)                                ccGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGc                                                                                                                  >  4:338330/1‑149 (MQ=255)                                ccGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGc                                                                                                                  >  7:219807/1‑149 (MQ=255)                                          ccAGTGGGCGCGAATACGCATTTTGGCCAAAGCGACGCCAGGACGTGTGTGTCCGCGGGTGGTCATTCCTTGCTGTTGGCATGAGTCACTGCTACGGCGCGGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCttt                                                                                                        <  8:277185/149‑1 (MQ=255)                                          ccAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACATGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCttt                                                                                                        >  7:17593/1‑149 (MQ=255)                                           cAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCtttt                                                                                                       <  2:282656/149‑1 (MQ=255)                                                 gcgcGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGc                                                                                                     >  5:320830/1‑145 (MQ=255)                                                    cgAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTTGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAg                                                                                              <  7:240003/149‑1 (MQ=255)                                                    cgAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAg                                                                                              <  1:163258/149‑1 (MQ=255)                                                     gAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAgg                                                                                             >  3:255313/1‑149 (MQ=255)                                                         aCGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCAtt                                                                                         <  5:353808/149‑1 (MQ=255)                                                            cAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGaa                                                                                      <  3:297345/149‑1 (MQ=255)                                                                 tGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGGTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCAt                                                                                 <  1:351812/149‑1 (MQ=255)                                                                     cAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTc                                                                             >  1:224067/1‑149 (MQ=255)                                                                     cAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTc                                                                             <  2:199897/149‑1 (MQ=255)                                                                          cGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCTGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCa                                                                        <  8:17593/149‑1 (MQ=255)                                                                                cAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCAtgc                                                                  >  7:134362/1‑149 (MQ=255)                                                                                cAGCACATGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGACGCACTGCAACGGAGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACTCTGCGCCCTTTTGCCAGATAGGCAGTGAACTCATCTTCCGGCACCAtgc                                                                  >  3:249351/1‑149 (MQ=255)                                                                                    aCCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGcc                                                              <  6:320830/149‑1 (MQ=255)                                                                                        gtgtgGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCTGATAGGCa                                                                                           >  5:328725/1‑116 (MQ=255)                                                                                        gtgtgGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCa                                                                                           <  6:328725/116‑1 (MQ=255)                                                                                            ggCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑Tc                                                    <  8:219807/149‑1 (MQ=255)                                                                                             gCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑Tcc                                                   >  5:176594/1‑149 (MQ=255)                                                                                             gCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑Tc                                                    <  8:349294/148‑1 (MQ=255)                                                                                                 cgGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCcaca                                               <  6:98765/149‑1 (MQ=255)                                                                                                  gggTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAg                                              <  2:224067/149‑1 (MQ=255)                                                                                                  gggTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAg                                              >  3:147028/1‑149 (MQ=255)                                                                                                        gCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACAt                                         >  3:183109/1‑148 (MQ=255)                                                                                                          aTTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATcac                                      <  6:176594/149‑1 (MQ=255)                                                                                                                     aTGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAgg                           <  3:338330/149‑1 (MQ=255)                                                                                                                         ccTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTg                       >  5:201707/1‑149 (MQ=255)                                                                                                                             aGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGca                     <  4:255313/147‑1 (MQ=255)                                                                                                                               gCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAg                  >  6:94428/1‑148 (MQ=255)                                                                                                                                   tGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCg                                                                           >  4:151638/1‑89 (MQ=255)                                                                                                                                   tGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCg                                                                           <  3:151638/89‑1 (MQ=255)                                                                                                                                     cAACGTCGCCGCGATGCGGCGCTCTCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCtgt           <  4:147028/149‑1 (MQ=255)                                                                                                                                      gcgagcgcCGCTTCGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCtgtg          >  3:54461/5‑149 (MQ=255)                                                                                                                                      gcgagcgcCGCTTCGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCtgtg          >  5:93157/5‑149 (MQ=255)                                                                                                                                          gcgcCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCtgt           <  7:247570/144‑1 (MQ=255)                                                                                                                                          gcgcCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCtgt           >  8:247570/1‑144 (MQ=255)                                                                                                                                              cgcgAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCCGCGTGTACCCCAGGACATAACAGCCTTCAAGGCTTGCACAGTACCGGTGGGAGCg    >  1:320080/1‑149 (MQ=255)                                                                                                                                               gcgAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCTGTGGGAGCggg  >  6:71734/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTGATGATCCTGCTTGGCTTTGCCCTGATGGCCGCACTGACCCAGTGGGCGCGAAAACGCAAAATGGCCAAAGCGACGCCAGCACCTGTGTGGCCGCGGGTGGGCATTCCTTGCTGATGGCCTGAGGCACTGCAACGGCGCCGCGAAGCGGCGCTCGCGGTTACGCGTTTGCCTGCACGCTGCGCCCTTTTGCCAGATAGGCATTGAACTCATCTTCCGGCACCATGCTGCCCCC‑CGT‑TCCCACAGGACATCACAGCCTTCAAGGCTTGCACAGTACCTGTGGGAGCGGG  >  NC_002947/3074989‑3075278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑