| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,590 | T→C | 21.5% | D1032D (GAT→GAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,590 | 0 | T | C | 21.5% | 197.8 / 49.9 | 104 | D1032D (GAT→GAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (36/45); new base C (12/10); total (48/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGAC > NC_002947/197442‑197728 | cccTGACGGGGAGCCCGTCGGTCACCGAAGGCTGCGTGATCACCTTCACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGAt < 8:176414/149‑1 (MQ=255)cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGAt > 4:237893/1‑149 (MQ=37) ccTGACGGCGAGCCCGTCGGTCACCGAATGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGAtt > 1:22694/1‑149 (MQ=37) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAACCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGAtt > 7:279777/1‑149 (MQ=37) tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGCTCCTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑ACGCCGGCAAGCCCCAGGGCAGCGTCGATTTc > 3:37757/1‑149 (MQ=255) tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTc > 7:143489/1‑149 (MQ=37) gACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc < 8:203432/149‑1 (MQ=37) gACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTc > 6:303422/1‑148 (MQ=37) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc < 2:61295/148‑1 (MQ=37) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCa < 5:47652/149‑1 (MQ=37) tCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGaa < 8:323376/149‑1 (MQ=37) tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc < 7:37546/149‑1 (MQ=37) tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc < 4:123083/149‑1 (MQ=37) aCCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTGCAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc < 1:96503/148‑1 (MQ=255) cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc < 6:226925/145‑1 (MQ=37) gAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa < 3:237893/149‑1 (MQ=37) gAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa < 2:255594/149‑1 (MQ=37) aaGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCAGGCGAATGACGTCTa > 6:311628/1‑148 (MQ=37) agacggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTaca < 4:91830/146‑1 (MQ=37) gcggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaac < 3:322098/149‑1 (MQ=37) ggcgTGATAACCTACACCGTGATACTGAGAAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAACCC‑‑ATTACCGTTG‑AAGCCGGAAAGACCCAGTGCAGCGTCGATTTAAAGACCCCGGCGAATTACGTCTacaaca > 4:314818/1‑148 (MQ=11) gcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACaacaac > 8:31444/1‑149 (MQ=37) atctaCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt < 4:95603/145‑1 (MQ=37) aTCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAATCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTc > 8:73756/1‑149 (MQ=37) tCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTc < 3:144028/148‑1 (MQ=37) aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAATCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa < 7:73756/148‑1 (MQ=37) cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATATTAAGGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTGTACAACAACGGTTCGAc > 1:121271/1‑149 (MQ=11) cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAccg > 4:2025/1‑147 (MQ=25) cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTg > 6:8384/1‑149 (MQ=38) accccctGATCCGTAGCCATCCGGCCCCGACGCCGGGAACCGTGACCCAGGCCAACGGCCAAACC‑‑ATTTCCCGTG‑TAGCCCGCAAGACCCAGGGGAGCGGCGATTTCCAGTCCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTc < 8:279777/143‑1 (MQ=11) acCGTGATCCTGAGCAATCCTGCGCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAg < 7:70239/149‑1 (MQ=21) acCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa > 6:51638/1‑142 (MQ=37) acCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa < 5:51638/142‑1 (MQ=37) aTCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCa < 1:241731/148‑1 (MQ=38) tCCTTAGCAATCCTGCCCAGACGCCTGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGt < 7:82553/145‑1 (MQ=255) tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACaacaac < 5:160802/128‑1 (MQ=35) tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAAAAACGGTTCGACTGTCAGCGTCAc > 3:193897/1‑148 (MQ=38) tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGCCAAGACCCAGGGCAGCCTCGATTTCCAGACCCCCGCCAATGACGTCTACAACAACGGATCCACTGTCAGCGTCAc > 7:106210/1‑148 (MQ=11) tCCTGACCAATCCAGACCAGACGCCGGTAACCGTGACCCTGTCCAAACCCCAAACC‑‑ATTACCGAAG‑AAGCCGGCAATACCCAGGGAAGCGACTATTTCCAGACCCCGCCGAAAGACTACAACAACAACCGAACGACTGTCAGctacac > 8:82553/1‑143 (MQ=9) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCa > 3:315664/1‑149 (MQ=17) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑ACGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCAt < 1:51870/149‑1 (MQ=37) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc > 2:198716/1‑149 (MQ=37) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc > 8:49903/1‑149 (MQ=17) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAATAACGGTTCGACTGTCAGCGTCACCATc < 4:193897/147‑1 (MQ=37) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCga < 5:303422/149‑1 (MQ=37) cAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCATTGC‑‑ATTACTGTTG‑AAGCCGGCAATACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAc < 6:152821/129‑1 (MQ=11) aTCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAAc < 3:368034/149‑1 (MQ=17) ccTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc < 7:129395/149‑1 (MQ=17) cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa < 4:233820/123‑1 (MQ=35) cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa > 3:233820/1‑123 (MQ=35) cccAGCCGCCGGTAACCGTGACCCTGGCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCTGGGCAGCGGCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCcaca < 6:342807/149‑1 (MQ=11) ccAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAg > 1:371562/1‑149 (MQ=37) gACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg < 1:215594/149‑1 (MQ=255) gACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg < 3:314818/149‑1 (MQ=37) aCGCCGGTGACCGTGACCCTCTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCATAACCCAGGGCATCGTCGACTTCCACACCCCGGCGATTGTAGTCTACAACAACGGTTCGACTGTCAGCCTCACCATCGAGAACGCCACAGGCgg < 7:53413/149‑1 (MQ=11) cGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCgg > 5:89803/1‑148 (MQ=17) ggTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACACAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCCCCATCGAGAACGCCCCAGGCGGTaaat > 5:99298/1‑147 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 2:375886/149‑1 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 4:186714/149‑1 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 4:16668/149‑1 (MQ=34) aCCGTGACACTGTCCAACGGCCAAACG‑‑ATTACAGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 5:95399/149‑1 (MQ=11) aCCGGGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 8:61753/149‑1 (MQ=18) cGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGc < 5:311628/149‑1 (MQ=32) tGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCaa < 7:31444/149‑1 (MQ=32) tGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCa > 5:155490/1‑148 (MQ=32) ccTGTCCAGCGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTgc > 8:285965/1‑148 (MQ=17) ccTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCCCCATCGAGAACGCCACACGCCGTAATTTCGAGCAACTGa > 2:331517/1‑149 (MQ=11) ccTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa > 2:327352/1‑149 (MQ=12) ccTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa > 2:147083/1‑149 (MQ=32) cTGTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc < 1:214442/149‑1 (MQ=12) tGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAcc > 6:129236/1‑149 (MQ=32) gTCCAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAccc > 3:193699/1‑149 (MQ=12) cAACGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGCCCCCGGCGAATGACGTCTACAACAACGGTACGACTGTCAGCGTCACCATCGAGAACGCCACAGGCCGTAATTTCCAGCAACTGACCCCg > 1:164017/1‑148 (MQ=11) aaCGGCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa > 7:132654/1‑149 (MQ=12) aaCGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa > 1:386604/1‑149 (MQ=32) ggCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACGATCGAGAACGCCCCAGGCGGTAATTTCGAGCACCTGGCGCCGAAccc > 8:176808/1‑149 (MQ=11) ggCCAAACG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAccc > 1:361278/1‑149 (MQ=255) ggCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAccc < 5:129236/149‑1 (MQ=34) aaaCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACg > 7:135766/1‑149 (MQ=35) aaaCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACg > 5:377222/1‑149 (MQ=35) aaCG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg > 7:235287/1‑116 (MQ=255) aaCG‑‑ATTACCGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCAACTGTCAGCGTCACCATCGAGAACGCCACAGGCg < 8:235287/116‑1 (MQ=11) aaCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGc < 5:8384/149‑1 (MQ=35) aaCC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGc > 8:115193/1‑149 (MQ=35) c‑‑ATTCCCGTTG‑AAGCCGGCAAGACCCAGGGTAGCGTCGTTTTCCAGATCCAGGCGAATGACGTCTACATCAAGGGTTCGTCTTTAAGGGTCAGCATCGTGAAGGCCACAGGCGGTAATTTCGTGGATCTGACCGCGAACCCGTCGCCgg < 4:37757/149‑1 (MQ=11) aTTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGc < 2:22694/149‑1 (MQ=37) ccGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGCACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt > 5:20761/1‑146 (MQ=37) ccGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCt < 4:40840/146‑1 (MQ=12) ccGTTG‑AAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTAGAGCAACTGACCCCGAACCCGACCCCGGCt > 3:40840/1‑146 (MQ=255) aaGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCAc > 1:223098/1‑148 (MQ=12) aGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGa > 5:362160/1‑149 (MQ=12) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGAt > 6:159000/1‑149 (MQ=37) ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATc < 7:8448/149‑1 (MQ=37) ccGGCAAAACCCAGGGCAGCGTCGACTTCCAGACACCGGCGAATGACGACTACAACAACGGTTCGACTGTCAGCGTCAACATCGAGAACGACACAGGCGGAAATTTCGAGCAACTCACCCCGTACACCACCCCGGCTCAGACCACGAt > 6:314615/1‑148 (MQ=11) ggCAAGACCCAGGGCAGCGTCGATTTACAGACCCCGGCGAATGACGTATACAACAACGGTTAGACTGTCAGCGTCACCATAGAGAACCCCACAGGCGGTAAATTCGAGCAACGGACACCGAACCCCACGCCGGCTCAGACCCCGATCAc > 2:349301/1‑149 (MQ=11) ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCaa < 2:361278/149‑2 (MQ=9) gCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCaac < 4:193699/149‑3 (MQ=2) cAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAcc > 7:122191/1‑148 (MQ=38) gCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGac < 7:285965/149‑1 (MQ=39) gCAGCGTCGAATTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCTACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTTACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGac < 6:20761/149‑1 (MQ=255) | CCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACC‑‑ATTACCGTTG‑AAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGAC > NC_002947/197442‑197728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |