Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,203,365	A→C	10.0%	Q39P (CAG→CCG) ‡	PP_2810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,203,365	0	A	C	10.0%	217.4 / 11.3	76	Q39P (CAG→CCG) ‡	PP_2810	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/35);  new base C (5/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCACTGGAAGT  >  NC_002947/3203218‑3203511                                                                                                                                                    |                                                                                                                                                    ccGGTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAg                                                                                                                                                    <  2:318531/149‑1 (MQ=255)  cGGTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAgg                                                                                                                                                   <  6:39387/149‑1 (MQ=255)  cGGTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAgg                                                                                                                                                   >  2:161192/1‑149 (MQ=255)    gTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAgggg                                                                                                                                                 <  1:374285/149‑1 (MQ=255)       cAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGc                                                                                                                                               <  7:111688/148‑1 (MQ=255)           cccTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCCTGGGGCGCAgc                                                                                                                                          <  3:230128/149‑2 (MQ=255)            ccTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGgc                                                                                                                                         <  1:161192/149‑1 (MQ=255)            ccTTCGTTTGAGGATCCTCCAATGAAACAACTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTCCGCCCCCtgggg                                                                                                                                                >  1:31029/1‑142 (MQ=255)            ccTTCGTTTGAGGATCCTCCAATGAAACAACTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTCCGCCCCCtgggg                                                                                                                                                <  2:31029/142‑1 (MQ=255)             cTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACACTTGCGGCCTCGACCGCGATCTGTTAGGTACAGGCTGCGCCCCAGGGGGCGGAGgcg                                                                                                                                        >  8:353801/1‑149 (MQ=255)                 ggttGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGCAGGCGCTgg                                                                                                                                    <  6:165522/147‑1 (MQ=255)                  tttGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTggg                                                                                                                                   >  5:335595/1‑149 (MQ=255)                  tttGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTggg                                                                                                                                   <  8:26816/149‑1 (MQ=255)                    tGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGtt                                                                                                                                 >  1:124709/1‑149 (MQ=255)                        gATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCg                                                                                                                               >  6:203484/1‑147 (MQ=255)                        gATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGaa                                                                                                                             <  7:3132/149‑1 (MQ=255)                            ctccAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACCGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGtt                                                                                                                          >  8:291337/1‑148 (MQ=255)                             tccAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGa                                                                                                                        >  1:261742/1‑149 (MQ=255)                             tccAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCAGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGa                                                                                                                        >  3:64851/1‑149 (MQ=255)                               cAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGAc                                                                                                                       <  2:85306/148‑1 (MQ=255)                                aaTGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGAcgc                                                                                                                     <  8:356957/149‑1 (MQ=255)                                       cAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTggg                                                                                                              >  3:179779/1‑149 (MQ=255)                                        aaGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTgggg                                                                                                             >  1:92135/1‑149 (MQ=255)                                         aGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTggggg                                                                                                            <  4:25134/149‑1 (MQ=255)                                             aCTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGCCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCga                                                                                                        <  2:124709/149‑1 (MQ=255)                                                 cACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAgc                                                                                                    >  2:333488/1‑149 (MQ=255)                                                 cACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAgc                                                                                                    >  6:137504/1‑149 (MQ=255)                                                   cGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGTTCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAgcg‑c                                                                                                 <  2:228009/149‑1 (MQ=255)                                                      tCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGcg‑ccgc                                                                                              >  5:216372/1‑149 (MQ=255)                                                        ggAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACTCCGCTGGGGGGCGAGAGcg‑ccgc                                                                                              >  6:117218/1‑147 (MQ=255)                                                         gAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGACGCTCGGGGGCGAGAGCG‑CCGCCAg                                                                                           >  3:119621/1‑149 (MQ=255)                                                               gtCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGATCGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑ACGCCAGTTCTAg                                                                                     >  3:153754/1‑149 (MQ=255)                                                                   tATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAgcggc                                                                                 <  7:152600/149‑1 (MQ=255)                                                                     tCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGa                                                                               <  4:131595/149‑1 (MQ=255)                                                                       cgcccgccGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTCTTCGGTACAGGCTGCGCACCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGata                                                                             >  4:238416/1‑149 (MQ=255)                                                                        gcccgccGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGatat                                                                            >  4:173075/1‑149 (MQ=255)                                                                                  ttttCAGTCAGCTGCTGGTTACCCTTGCGGAGGCGACCGCGATCTGTTGGTTCCAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGTTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTg                                                                   <  4:94178/148‑1 (MQ=255)                                                                                  ttttCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTgg                                                                  >  6:359176/1‑149 (MQ=255)                                                                                  ttttCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTgg                                                                  >  4:137268/1‑149 (MQ=255)                                                                                  ttttCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTg                                                                   <  2:92135/148‑1 (MQ=255)                                                                                   tttCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTgg                                                                  <  1:333488/148‑1 (MQ=255)                                                                                    ttCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTggg                                                                 >  1:318091/1‑148 (MQ=255)                                                                                    ttCAGGCAGCTGCTGGTTACCCTTGCGGCCGCGACCGCGATCTGTGCGGTCCAGGCTGCGCCCCAGGTGGCGGAGGCGCTGGGTTCGAAGGTGCCCCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTggg                                                                 <  5:858/148‑1 (MQ=255)                                                                                        ggCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAg                                                            <  3:304567/149‑1 (MQ=255)                                                                                         gCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTgg                                                                  >  1:142722/1‑142 (MQ=255)                                                                                         gCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTgg                                                                  <  2:142722/142‑1 (MQ=255)                                                                                         gCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGc                                                           >  5:79600/1‑149 (MQ=255)                                                                                               gctgGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAggg                                                      >  4:263062/1‑148 (MQ=255)                                                                                                      aCCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCATGAGGGCGACTGGGCCCGCCATTTATAGCGGCGATATACAACCATGGCCACAGCAAGGGCAGAAgg                                               >  1:320655/1‑149 (MQ=255)                                                                                                         cTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCAGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGc                                           <  4:64851/149‑1 (MQ=255)                                                                                                              ggCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGAt                                      >  8:351064/1‑149 (MQ=255)                                                                                                                ccTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGa                                       >  3:95156/1‑146 (MQ=255)                                                                                                                 cTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATg                                     >  3:170968/1‑147 (MQ=255)                                                                                                                 cTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGa                                   <  5:359176/149‑1 (MQ=255)                                                                                                                    gACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAg                                  <  6:335595/147‑1 (MQ=255)                                                                                                                    gACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAg                                  <  3:173075/147‑1 (MQ=255)                                                                                                                          gATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCATGGGCACAGCCAGGCCAGCAGGGGGCAGGAGGCAGTTACGGc                           >  8:23656/1‑148 (MQ=255)                                                                                                                                ttCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAg                        <  7:351064/145‑1 (MQ=255)                                                                                                                                ttCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGt                      <  3:263062/147‑1 (MQ=255)                                                                                                                                ttCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGt                      <  6:79600/147‑1 (MQ=255)                                                                                                                                ttCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTAc                    <  8:318464/149‑1 (MQ=255)                                                                                                                                ttCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATACCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTAc                    >  6:365573/1‑149 (MQ=255)                                                                                                                                     tACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAggg               <  7:291337/149‑1 (MQ=255)                                                                                                                                       cAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGa             <  5:117218/149‑1 (MQ=255)                                                                                                                                       cAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGGTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGa             <  5:203484/149‑1 (MQ=255)                                                                                                                                         ggCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAggggg                                            <  7:162066/116‑1 (MQ=255)                                                                                                                                         ggCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAggggg                                            >  8:162066/1‑116 (MQ=255)                                                                                                                                          cctccgcCCCCTGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCa          >  8:313866/5‑149 (MQ=255)                                                                                                                                           ctccgcCCCCTGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAg                        <  6:77451/131‑1 (MQ=255)                                                                                                                                           ctccgcCCCCTGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAg                        >  5:77451/4‑134 (MQ=255)                                                                                                                                           ctccgcCCCCTGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCAc         >  2:98625/4‑149 (MQ=255)                                                                                                                                           ctccgcCCCCTGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCAc         >  3:340525/4‑149 (MQ=255)                                                                                                                                                ccccAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATACCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCACTGGaa    <  5:365573/149‑1 (MQ=255)                                                                                                                                                 cccAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCTGGATGGAGTTACGGCCAGGTACGAGGGGAGAACTGGAAg   >  7:276112/1‑149 (MQ=255)                                                                                                                                                 cccAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCACTGGAAg   >  1:64659/1‑149 (MQ=255)                                                                                                                                                  ccAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATAGAGTTACGGCCAGGTACGAGGGGAGCACTGGAAGt  <  4:95156/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCGGTCCAAGCCCTTCGTTTGAGGATCCTCCAATGAAACAAGTCACTGCACGGTCGGAAAGTGTCGTATCCGCCCGCCGTATTTTCAGGCAGCTGCTGGTTACCCTTGCGGCCTCGACCGCGATCTGTTCGGTACAGGCTGCGCCCCAGGGGGCGGAGGCGCTGGGTTCGAAGTTGACGCCGCTGGGGGGCGAGAGCG‑CCGCCAGTTCTAGCGGCGATATTCCAGCCTGGGCACAGCCAGGGCAGCAGGGGGCAGGATGGAGTTACGGCCAGGTACGAGGGGAGCACTGGAAGT  >  NC_002947/3203218‑3203511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑