Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,227,928 T→C 9.1% V256A (GTA→GCA)  PP_2828 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,227,9280TC 9.1% 117.1 / 3.2 44V256A (GTA→GCA) PP_2828hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base C (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGC  >  NC_002947/3227781‑3228075
                                                                                                                                                    |                                                                                                                                                    
gggCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTa                                                                                                                                                     >  2:165074/1‑149 (MQ=255)
  gCCAGGCGCAGGCTGCGCCGGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTAcc                                                                                                                                                   >  4:279735/1‑149 (MQ=255)
    ccGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGCACCgg                                                                                                                                                 >  5:339911/1‑149 (MQ=255)
       gcgcAGCCTGCGCCTGGCCGACGTTCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGc                                                                                                                                              <  8:185442/149‑1 (MQ=255)
           aGCCTGCGCCTGGGCGGCGTGCATGCGCCGCATGCAGTGCACCAGTGCGTGGGGGCGCTGGCCCATGG‑GATGAGCCGATTGGACGGTGCTGTTGGGGGCGCACCGGGTGGTCTGCACGAGCCGGTTCCAGGCGGCGTACGGGAGCGTgc                                                                                                                                          <  1:271713/149‑1 (MQ=255)
                    cgggccggccTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑g                                                                                                                                <  3:279735/147‑1 (MQ=255)
                      ggCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGCACCGGCGCACGCTCCGGTAC‑GGc                                                                                                                              <  6:339911/149‑1 (MQ=255)
                       gCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGTCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑gg                                                                                                                               <  4:104382/147‑1 (MQ=255)
                           gCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGCACCGGCACACGCTCCGGTAC‑GGcgcctg                                                                                                                         <  6:73469/149‑6 (MQ=255)
                            actGCATGCGGGGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCg                                                                                                                        >  8:293935/3‑149 (MQ=255)
                             gggcttGCCCCGCAGGCAGTGCTCCAGTGCCTGGCGGCGCGGGCCCAAGG‑GCTGAGCCGTTTGCACGGTGCTGTTGGGGGCGCACCGGGGGGTCAGTACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGc                                                                                                                       <  4:268392/144‑1 (MQ=255)
                                     cccGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATtgat                                                                                                                >  6:307693/1‑148 (MQ=255)
                                     cccGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATtgat                                                                                                                >  1:8289/1‑148 (MQ=255)
                                            gCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGtt                                                                                                        >  5:351246/1‑149 (MQ=255)
                                                   aCCAGTGCCTGGCGGCGCTGGCCCAAGGTGCTGAGCCGATTGCACGGTGGTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTCCCGGAGCGTGCGCCGGTGC‑GGCTGATCGGTATTGATGACCTGTTGCCgag                                                                                                  >  1:221776/1‑147 (MQ=255)
                                                   aCCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGGCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGcc                                                                                                     <  2:221776/145‑1 (MQ=255)
                                                         gCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGGCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCActtgctt                                                                                           <  8:276189/149‑1 (MQ=255)
                                                           cTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGa                                                                                         <  7:293935/149‑1 (MQ=255)
                                                              gcggcgCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAaga                                                                                      <  5:12445/149‑1 (MQ=255)
                                                                 gcgcTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAagag                                                                                     >  2:166565/1‑147 (MQ=255)
                                                                    cTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGCGGGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGagcagc                                                                                 <  1:85662/149‑1 (MQ=255)
                                                                    cTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGGGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGagcagca                                                                                <  8:320412/149‑1 (MQ=255)
                                                                         ccAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGagca                                                                                   <  5:264128/141‑1 (MQ=255)
                                                                         ccAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGagca                                                                                   >  6:264128/1‑141 (MQ=255)
                                                                         ccAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCtt                                                                            >  8:319776/1‑148 (MQ=255)
                                                                                    aGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCt                                                                 >  3:78533/1‑149 (MQ=255)
                                                                                      ccGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTgg                                                               <  1:166565/149‑1 (MQ=255)
                                                                                       cGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTggg                                                              <  1:165074/149‑1 (MQ=255)
                                                                                        gATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGt                                                             >  1:269000/1‑149 (MQ=255)
                                                                                            gCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGt                                                          <  4:78533/148‑1 (MQ=255)
                                                                                                 gTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGTACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTc                                                    <  5:307693/149‑1 (MQ=255)
                                                                                                  tGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTc                                                    >  3:120294/1‑148 (MQ=255)
                                                                                                               gcACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGa                                      >  5:127967/1‑149 (MQ=255)
                                                                                                               gcACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGa                                      >  7:339365/1‑149 (MQ=255)
                                                                                                                cACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGAt                                     <  4:195811/149‑1 (MQ=255)
                                                                                                                 aCCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATg                                    >  7:102057/1‑149 (MQ=255)
                                                                                                                    gggTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCtt                                 <  2:8289/149‑1 (MQ=255)
                                                                                                                              cACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGc                              >  4:212273/1‑142 (MQ=255)
                                                                                                                              cACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGc                              <  3:212273/142‑1 (MQ=255)
                                                                                                                                     cGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACTGCTGCGTATGCTTTGCCGCTGTCCGAATtc                <  7:59443/149‑1 (MQ=255)
                                                                                                                                           cAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGa           <  2:113078/148‑1 (MQ=255)
                                                                                                                                            atcagcCGCACCGGCGCACGCTCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACt         >  3:276967/5‑149 (MQ=255)
                                                                                                                                             ggCGCCGTACCGGAGCGTGCGCCGGTGC‑TGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTc        <  8:351607/149‑1 (MQ=255)
                                                                                                                                             ggCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGTATGCTTTGCCGCTGTCCTAATTCGTCGACTc        <  6:351246/149‑1 (MQ=255)
                                                                                                                                                    tACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGc  >  5:136045/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGG‑GCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGCGTGCGCCGGTGC‑GGCTGATCGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGC  >  NC_002947/3227781‑3228075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑