Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,256,604 G→A 8.8% R184C (CGC→TGC)  PP_2853 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,256,6040GA 8.8% 125.6 / 2.5 46R184C (CGC→TGC) PP_2853hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

ACGTTGATATCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATCGTAATGGGTATAGG  >  NC_002947/3256471‑3256747
                                                                                                                                     |                                                                                                                                                 
aCGTTGATATCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTtata                                                                                                                                    <  2:241354/149‑1 (MQ=255)
   ttGATATCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCg                                                                                                                                 <  1:210344/149‑1 (MQ=255)
     gATATCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCgg                                                                                                                                <  4:111069/148‑1 (MQ=255)
         tCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGt                                                                                                                           >  5:146509/1‑149 (MQ=255)
           gCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCa                                                                                                                         >  1:169560/1‑149 (MQ=255)
           gCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACGACCGGGCCGGGGATGACGAACTGCCAGCGCTGCGGCAGGTTATAGCgggacggcc                                                                                                                         >  4:36679/1‑143 (MQ=255)
            ccGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑t                                                                                                                      >  5:232365/1‑149 (MQ=255)
             cGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCTCTGCGCCAGGTTATAGCGGGCAGTCA‑‑Tg                                                                                                                     <  6:2287/149‑1 (MQ=255)
              gATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGt                                                                                                                    <  3:382027/149‑1 (MQ=255)
                 ttCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGG‑‑CCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGACA‑‑TGTCCAAg                                                                                                               >  1:347635/1‑149 (MQ=255)
                   ccGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCaa                                                                                                                <  8:53062/148‑1 (MQ=255)
                     gggTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGt                                                                                                             <  6:232365/149‑1 (MQ=255)
                             ggTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGt                                                                                                     >  1:343411/1‑149 (MQ=255)
                                 aCGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGc                                                                                                 <  2:169560/149‑1 (MQ=255)
                                  cGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGcc                                                                                                >  3:160280/1‑149 (MQ=255)
                                                       ccccGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTc                                                                                     <  5:122995/139‑1 (MQ=255)
                                                       ccccGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTc                                                                                     >  6:122995/1‑139 (MQ=255)
                                                        cccGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAACCTCGACCGGATCAAGGCTGacaa                                                                          <  1:744/149‑4 (MQ=255)
                                                                cccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTc                                                                                     <  1:347936/130‑1 (MQ=255)
                                                                cccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTc                                                                                     >  2:347936/1‑130 (MQ=255)
                                                                cccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGACA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAgg                                                                  <  2:347635/149‑1 (MQ=255)
                                                                 ccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAg                                                                                                                                          >  3:382104/1‑78 (MQ=255)
                                                                 ccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAg                                                                                                                                          <  4:382104/78‑1 (MQ=255)
                                                                 ccccGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAgg                                                                  >  5:323722/1‑148 (MQ=255)
                                                                          accGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCATCTGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGa                                                          <  1:111214/149‑1 (MQ=255)
                                                                           ccGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGGTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTc                                                       <  6:146509/149‑1 (MQ=255)
                                                                              gACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAg                                                    >  1:377964/1‑149 (MQ=255)
                                                                               aCGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGt                                                   <  4:160280/149‑1 (MQ=255)
                                                                                  aGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGcc                                                <  2:343411/149‑1 (MQ=255)
                                                                                       gTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGa                                                                    <  7:89284/124‑1 (MQ=255)
                                                                                       gTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGa                                                                    >  8:89284/1‑124 (MQ=255)
                                                                                       gTGGACTCGCGGTAAACTACGGGGAAGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGa                                             >  4:193446/1‑147 (MQ=255)
                                                                                               gcgGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCACTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGtt                                                                               >  2:151432/1‑105 (MQ=255)
                                                                                               gcgGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCACTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGtt                                                                               >  2:145919/1‑105 (MQ=255)
                                                                                               gcgGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCACTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGtt                                                                               <  1:151432/105‑1 (MQ=255)
                                                                                               gcgGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCACTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGtt                                                                               <  1:145919/105‑1 (MQ=255)
                                                                                                cgGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAg                                  >  1:21007/1‑149 (MQ=255)
                                                                                                 ggTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAgg                                 <  1:12486/149‑1 (MQ=255)
                                                                                                  gTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAggg                                >  8:53405/1‑149 (MQ=255)
                                                                                                       cTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAgc                           <  3:169869/149‑1 (MQ=255)
                                                                                                               aCGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGt                   <  7:53405/149‑1 (MQ=255)
                                                                                                                 gTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTAt                 >  5:130247/1‑149 (MQ=255)
                                                                                                                      tGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATc                <  7:25057/145‑1 (MQ=255)
                                                                                                                      tGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATc                >  8:25057/1‑145 (MQ=255)
                                                                                                                              tGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATCGTAATGGGtt      >  7:193154/1‑146 (MQ=255)
                                                                                                                                ccAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATCGTAATGGGTATAgg  >  7:357374/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                                 
ACGTTGATATCGCCGATTTCCGGGTCGCGGGTCACGCTGGCATCGGAAGTGGTTGCCCCGGCCCCCCCCGCACCACCGGACGAGTAGGTGGACTCGCGGTAAACTACGGGGACGTTGATGTCGAACTGCCAGCGCTGCGCCAGGTTATAGCGGGCAGTCA‑‑TGTCCAAGGTCCAGTTGTCAGCCTTGATCCGGTCGAGGTTGATGCTGCCGAGGAAGATCGAGTCCAGTGCCAGAAAGCCATTGAGGGTTAGCGCACGGGTATCGTAATGGGTATAGG  >  NC_002947/3256471‑3256747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑