Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,298,028 G→C 8.9% G82A (GGT→GCT)  pvdQ → acyl‑homoserine lactone acylase PvdQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,298,0280GC 8.9% 166.6 / 6.4 56G82A (GGT→GCT) pvdQacyl‑homoserine lactone acylase PvdQ
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base C (3/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

GCCGACGCCAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTG  >  NC_002947/3297880‑3298176
                                                                                                                                                    |                                                                                                                                                    
gCCGACGCCAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCgtgagcgg                                                                                                                                                      >  5:354604/1‑149 (MQ=255)
 ccGACGCCAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCgtgagcggt                                                                                                                                                     >  8:344278/1‑149 (MQ=255)
        cAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGAGCGTGAGCGCTCACCGCt                                                                                                                                              >  1:72975/1‑149 (MQ=255)
               cAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCAATGCCTGCTAGCGAATGAAGTGCTGACCGAGAGCGGTGAGCGCTCACGCTa                                                                                                                                       >  6:109675/1‑149 (MQ=255)
                 gATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGAGCGTGAGCGCTCACCGCTCACggtcag                                                                                                                                     <  2:72975/149‑6 (MQ=255)
                  aTCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGCTCACCGCTCACg                                                                                                                                          >  8:280374/1‑143 (MQ=255)
                  aTCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGCTCACCGCTCACg                                                                                                                                          <  7:280374/143‑1 (MQ=255)
                   tCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTc                                                                                                                                   <  1:171538/149‑1 (MQ=255)
                      gCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGgc                                                                                                                                <  2:140234/149‑1 (MQ=255)
                      gCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGgc                                                                                                                                <  5:177309/149‑1 (MQ=255)
                          taCCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGcc                                                                                                                             <  8:103748/148‑1 (MQ=255)
                               gTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCTGTGAGCGCTCACGCTATTTCGGCGCCAATGGc                                                                                                                       <  4:73147/149‑1 (MQ=255)
                               gTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGc                                                                                                                       <  2:383096/149‑1 (MQ=255)
                                ttACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGcc                                                                                                                      <  8:213815/149‑1 (MQ=255)
                                          ccGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCGATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGa                                                                                                            <  2:376856/149‑1 (MQ=255)
                                            gCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGag                                                                                                           >  8:206016/1‑148 (MQ=255)
                                            gCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGag                                                                                                           >  7:202893/1‑148 (MQ=255)
                                                 tCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGt                                                                                                     >  6:91757/1‑149 (MQ=255)
                                                    tGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGAc                                                                                                  <  7:33037/149‑1 (MQ=255)
                                                        cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc                                                                                              >  5:284468/1‑149 (MQ=255)
                                                        cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc                                                                                              >  1:139284/1‑149 (MQ=255)
                                                        cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc                                                                                              >  1:127606/1‑149 (MQ=255)
                                                         aaGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCt                                                                                             <  5:91757/149‑1 (MQ=255)
                                                           ggATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTgg                                                                                           >  4:265089/1‑149 (MQ=255)
                                                                        cTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGGGCAGCGTGCGAGCCTGGCCAGCGACCTggt                                                                              >  8:256448/1‑147 (MQ=255)
                                                                           ggCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGttc                                                                             <  3:356893/147‑1 (MQ=255)
                                                                                tGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCt                                                                      >  6:277305/1‑149 (MQ=255)
                                                                                  gCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTgg                                                                    <  7:206016/149‑1 (MQ=255)
                                                                                        gCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCaa                                                               >  3:371587/1‑148 (MQ=255)
                                                                                            tGCCTACGCCCAGGACAACCTTTGCCTTCTCGCGAATGAAGTGCTGAACTTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGTCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGcc                                                          <  5:109675/149‑1 (MQ=255)
                                                                                            tGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGcc                                                          <  7:344278/149‑1 (MQ=255)
                                                                                               cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg                                                       >  7:327877/1‑149 (MQ=255)
                                                                                               cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg                                                       >  3:54916/1‑149 (MQ=255)
                                                                                               cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGATGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg                                                       >  7:149223/1‑149 (MQ=255)
                                                                                                           cAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGAt                                            <  4:54916/148‑1 (MQ=255)
                                                                                                            aaCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGc                                          >  4:167442/1‑149 (MQ=255)
                                                                                                            aaCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGc                                          >  4:173348/1‑149 (MQ=255)
                                                                                                             aCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGcc                                         <  8:202893/149‑1 (MQ=255)
                                                                                                              ccTATGGCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCGGGAGCAGCGTGACAACGTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCt                                        <  7:239693/149‑1 (MQ=255)
                                                                                                              ccTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCt                                        >  4:132246/1‑149 (MQ=255)
                                                                                                                tATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTc                                      >  6:271801/1‑149 (MQ=255)
                                                                                                                  tGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTcc                                     <  5:271801/148‑1 (MQ=255)
                                                                                                                     cTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTg                                   >  8:236170/1‑147 (MQ=255)
                                                                                                                     cTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTg                                   >  8:43059/1‑147 (MQ=255)
                                                                                                                        cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAggt                              <  5:277305/149‑2 (MQ=255)
                                                                                                                        cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAg                                <  2:127606/147‑1 (MQ=255)
                                                                                                                        cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgc                              >  8:181270/1‑149 (MQ=255)
                                                                                                                         tCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcg                             >  3:329615/1‑149 (MQ=255)
                                                                                                                         tCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCAGCAGgcg                             >  5:124057/1‑149 (MQ=255)
                                                                                                                           gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc                            >  6:393751/1‑148 (MQ=255)
                                                                                                                           gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc                            >  3:221340/1‑148 (MQ=255)
                                                                                                                           gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGATCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc                            >  3:316505/1‑148 (MQ=255)
                                                                                                                                      aaatagcGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCa                                 <  7:187819/126‑1 (MQ=255)
                                                                                                                                      aaatagcGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCACAGCGGCGGTCGATGCCTTCCTGCa                                 >  8:187819/7‑132 (MQ=255)
                                                                                                                                       cTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGt               <  4:371587/149‑1 (MQ=255)
                                                                                                                                        atagcGTGAGCGCTCACCGCTCACGGTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTg              >  8:62489/5‑149 (MQ=255)
                                                                                                                                           ccGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCACCCCGCGTCGGTGCAg           >  5:67739/1‑149 (MQ=255)
                                                                                                                                            cGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAgg          >  5:360894/1‑149 (MQ=255)
                                                                                                                                                    gtgagcgCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTg  >  3:46079/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGACGCCAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTG  >  NC_002947/3297880‑3298176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑