| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,298,030 | G→C | 8.9% | E83Q (GAG→CAG) ‡ | pvdQ → | acyl‑homoserine lactone acylase PvdQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,298,030 | 0 | G | C | 8.9% | 165.6 / 5.0 | 56 | E83Q (GAG→CAG) ‡ | pvdQ | acyl‑homoserine lactone acylase PvdQ |
| Reads supporting (aligned to +/- strand): ref base G (29/22); new base C (3/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTGG > NC_002947/3297888‑3298177 | cAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGAGCGTGAGCGCTCACCGCt > 1:72975/1‑149 (MQ=255) cAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCAATGCCTGCTAGCGAATGAAGTGCTGACCGAGAGCGGTGAGCGCTCACGCTa > 6:109675/1‑149 (MQ=255) gATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGAGCGTGAGCGCTCACCGCTCACggtcag < 2:72975/149‑6 (MQ=255) aTCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGCTCACCGCTCACg > 8:280374/1‑143 (MQ=255) aTCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGCTCACCGCTCACg < 7:280374/143‑1 (MQ=255) tCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTc < 1:171538/149‑1 (MQ=255) gCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGgc < 5:177309/149‑1 (MQ=255) gCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGgc < 2:140234/149‑1 (MQ=255) taCCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGcc < 8:103748/148‑1 (MQ=255) gTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCTGTGAGCGCTCACGCTATTTCGGCGCCAATGGc < 4:73147/149‑1 (MQ=255) gTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGc < 2:383096/149‑1 (MQ=255) ttACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGcc < 8:213815/149‑1 (MQ=255) ccGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCGATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGa < 2:376856/149‑1 (MQ=255) gCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGag > 8:206016/1‑148 (MQ=255) gCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGag > 7:202893/1‑148 (MQ=255) tCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGt > 6:91757/1‑149 (MQ=255) tGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGAc < 7:33037/149‑1 (MQ=255) cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc > 5:284468/1‑149 (MQ=255) cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc > 1:139284/1‑149 (MQ=255) cAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAAcc > 1:127606/1‑149 (MQ=255) aaGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCt < 5:91757/149‑1 (MQ=255) ggATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTgg > 4:265089/1‑149 (MQ=255) cTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGGGCAGCGTGCGAGCCTGGCCAGCGACCTggt > 8:256448/1‑147 (MQ=255) ggCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGttc < 3:356893/147‑1 (MQ=255) tGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCt > 6:277305/1‑149 (MQ=255) gCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTgg < 7:206016/149‑1 (MQ=255) gCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCaa > 3:371587/1‑148 (MQ=255) tGCCTACGCCCAGGACAACCTTTGCCTTCTCGCGAATGAAGTGCTGAACTTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGTCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGcc < 5:109675/149‑1 (MQ=255) tGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGcc < 7:344278/149‑1 (MQ=255) cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg > 7:327877/1‑149 (MQ=255) cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg > 3:54916/1‑149 (MQ=255) cTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGATGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAg > 7:149223/1‑149 (MQ=255) cAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGAt < 4:54916/148‑1 (MQ=255) aaCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGc > 4:173348/1‑149 (MQ=255) aaCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGc > 4:167442/1‑149 (MQ=255) aCCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGcc < 8:202893/149‑1 (MQ=255) ccTATGGCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCGGGAGCAGCGTGACAACGTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCt < 7:239693/149‑1 (MQ=255) ccTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCt > 4:132246/1‑149 (MQ=255) tATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTc > 6:271801/1‑149 (MQ=255) tGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTcc < 5:271801/148‑1 (MQ=255) cTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTg > 8:236170/1‑147 (MQ=255) cTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTg > 8:43059/1‑147 (MQ=255) cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAggt < 5:277305/149‑2 (MQ=255) cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAg < 2:127606/147‑1 (MQ=255) cTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgc > 8:181270/1‑149 (MQ=255) tCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcg > 3:329615/1‑149 (MQ=255) tCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCAGCAGgcg > 5:124057/1‑149 (MQ=255) gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc > 6:393751/1‑148 (MQ=255) gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc > 3:221340/1‑148 (MQ=255) gCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGATCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGgcgc > 3:316505/1‑148 (MQ=255) aaatagcGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCa < 7:187819/126‑1 (MQ=255) aaatagcGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCACAGCGGCGGTCGATGCCTTCCTGCa > 8:187819/7‑132 (MQ=255) cTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGt < 4:371587/149‑1 (MQ=255) atagcGTGAGCGCTCACCGCTCACGGTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTg > 8:62489/5‑149 (MQ=255) ccGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCACCCCGCGTCGGTGCAg > 5:67739/1‑149 (MQ=255) cGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAgg > 5:360894/1‑149 (MQ=255) gtgagcgCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTg > 3:46079/1‑149 (MQ=255) tgagcgCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTgg < 7:236170/149‑1 (MQ=255) | CAGTGCCCAGATCCGCTATACCCGTTACGGCGTACCGCATATCGTGGCCAAGGATGAGCGTGGCCTGGGCTATGGCGTCGGCTATGCCTACGCCCAGGACAACCTATGCCTGCTCGCCAATGAAGTGCTGACCGTGAGCGGTGAGCGCTCACGCTATTTCGGCGCCAAGGGCCAGACCCTGGAGCAGCGTGACAACCTGGCCAGCGACCTGTTCTTCACCTGGCTCAATAGCCCAGCGGCGGTCGATGCCTTCCTGCAGGCGCAACCCGCGTCGGTGCAGGCATTGCTGG > NC_002947/3297888‑3298177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |