Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,857 A→T 7.5% I17N (ATT→AAT)  lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8570AT 7.5% 217.0 / 5.1 76I17N (ATT→AAT) lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base T (4/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAT  >  NC_002947/3300709‑3300992
                                                                                                                                                    |                                                                                                                                         
gCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGaaa                                                                                                                                           <  8:299301/149‑1 (MQ=255)
      ccGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCg                                                                                                                                     >  2:274875/1‑149 (MQ=255)
             aTGGCCTGGCAGATCGAGTGCTGGCAGGGCGGCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCACGGATGTACAGGGCCTTGGCTTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGttt                                                                                                                              <  6:209747/149‑2 (MQ=255)
             aTGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                              <  5:289217/149‑1 (MQ=255)
                  cTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTTGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                              >  6:366057/1‑144 (MQ=255)
                  cTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTTGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                              <  5:366057/144‑1 (MQ=255)
                   tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                        >  2:169935/1‑149 (MQ=255)
                     gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                        >  1:115866/1‑147 (MQ=255)
                     gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                        <  6:201449/147‑1 (MQ=255)
                        gATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGt                                                                                                                 >  2:305854/1‑149 (MQ=255)
                         aTCGACTGCTGGAAGGGCGTCATTGCATCGTCTCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTg                                                                                                                <  6:267179/149‑1 (MQ=255)
                          tCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                    >  1:242380/1‑130 (MQ=255)
                          tCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                    <  2:242380/130‑1 (MQ=255)
                             aCTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTg                                                                                                                >  2:100375/1‑145 (MQ=255)
                              ctgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                           <  6:160730/149‑1 (MQ=255)
                               tgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                           >  1:352078/1‑148 (MQ=255)
                                 ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcag                                                                                                        <  4:53818/149‑1 (MQ=255)
                                 ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                           >  5:209599/1‑146 (MQ=255)
                                    gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagag                                                                                                      <  6:248222/148‑1 (MQ=255)
                                     aaGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCATGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                         >  5:41429/1‑144 (MQ=255)
                                      aGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagat                                                                                                    >  7:65451/1‑148 (MQ=255)
                                       gggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCTGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatca                                                                                                  <  1:274875/149‑1 (MQ=255)
                                        ggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 <  6:278809/149‑1 (MQ=255)
                                         gCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 >  5:123679/1‑148 (MQ=255)
                                          cGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 >  6:170369/1‑147 (MQ=255)
                                           gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGGAGATCTAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                         <  2:338035/138‑1 (MQ=255)
                                           gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                         >  1:338035/1‑138 (MQ=255)
                                               tGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAAt                                                                                           >  1:245/1‑148 (MQ=255)
                                                  cATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACt                                                                                       <  1:169935/149‑1 (MQ=255)
                                                      gtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                   >  7:84466/1‑149 (MQ=255)
                                                       tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                     >  5:387437/1‑146 (MQ=255)
                                                       tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                     <  6:387437/146‑1 (MQ=255)
                                                         gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 <  1:62723/132‑1 (MQ=255)
                                                         gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 >  2:62723/1‑132 (MQ=255)
                                                         gCCAATCCACGCTTCGAAGAAGTCACCAAGGAGGTACAGGGCCTTGGCATGGCGGGGGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                         >  8:248323/1‑124 (MQ=255)
                                                         gCCAATCCACGCTTCGAAGAAGTCACCAAGGACGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                         <  7:248323/124‑1 (MQ=255)
                                                            aaTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCTGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGTGAAATATCCGGGCGTTCTTCTTGCA‑‑GGAGCAGATCAGAGATCAGCAGAATCACTCAATGAactc                                                                             >  2:225200/1‑149 (MQ=255)
                                                             aTCCACGCTTCGAAGAATTCACAAAGGATGTACAGGGCCTTGGCATGGCGCTCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCAGCGCGTTCTTCTTGAA‑‑GGTCCAGAACAGAGATCAGCAGAATcaatcaatatatcg                                                                             >  6:377985/1‑142 (MQ=255)
                                                             aTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCaa                                                                                    <  5:377985/141‑1 (MQ=255)
                                                                cACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCt                                                                         <  5:170369/149‑1 (MQ=255)
                                                                  cGCTTCGAAGAAGTCACCAAGGTTGTACATGGCCTTGGCATGGCTGGCGCGGCCATCGAGGAGATCAAGAAACGCCCGGGAAATATCCGTGCGTTCTTCTTGCA‑‑GGTGCATATCAGAGATCAGCAGAATCACTCAATGATCTCGGCttt                                                                       <  1:225200/149‑1 (MQ=255)
                                                                       ctaagaagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGTCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                    <  5:346085/145‑1 (MQ=255)
                                                                        gtagaagTCACCAAGGATGTACAGGGCCTTTGCATTGGGGGCGCGGCCATCGACCATATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCaga                                                                                                            <  8:140945/104‑1 (MQ=255)
                                                                        gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTGTTGCA‑‑GGTGCaga                                                                                                            >  7:140945/1‑106 (MQ=255)
                                                                        gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑TGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                 <  8:65451/149‑1 (MQ=255)
                                                                        gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                 >  7:127874/1‑149 (MQ=255)
                                                                        gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                 >  5:38208/1‑149 (MQ=255)
                                                                            aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                             <  6:123679/149‑1 (MQ=255)
                                                                               tCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcg                                                          >  3:37544/1‑149 (MQ=255)
                                                                                cACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTGCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgt                                                         >  4:162472/1‑149 (MQ=255)
                                                                                  ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 <  4:51389/107‑1 (MQ=255)
                                                                                  ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                 >  3:51389/1‑107 (MQ=255)
                                                                                    aaGGATGTACAGGGCCTTGGCATTGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCACCGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcg                                                       <  1:305854/149‑1 (MQ=255)
                                                                                    aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                             <  4:83418/141‑1 (MQ=255)
                                                                                    aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                             >  3:83418/1‑141 (MQ=255)
                                                                                    aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                     <  6:41429/149‑1 (MQ=255)
                                                                                     aGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTcc                                                    <  3:195089/149‑1 (MQ=255)
                                                                                        aTGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                 <  2:115866/149‑1 (MQ=255)
                                                                                        aTGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                 <  3:43511/149‑1 (MQ=255)
                                                                                           tACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGt                                              <  6:38208/149‑1 (MQ=255)
                                                                                             cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                            >  2:149139/1‑149 (MQ=255)
                                                                                             cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                            <  2:352078/149‑1 (MQ=255)
                                                                                                ggCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATAAAGAAACGCCAGGGAAATATCAGGTCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATAAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                         >  4:225125/1‑149 (MQ=255)
                                                                                                 gCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTcc                                        <  2:245/149‑1 (MQ=255)
                                                                                                      ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtggt                                    <  8:84466/148‑1 (MQ=255)
                                                                                                               ggcgCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCttt                          <  8:127874/149‑1 (MQ=255)
                                                                                                                    ggCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAAc                     >  5:118423/1‑149 (MQ=255)
                                                                                                                        aTCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTACTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGt                 >  2:196709/1‑149 (MQ=255)
                                                                                                                         tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                <  6:209599/149‑1 (MQ=255)
                                                                                                                           gAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTACTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTgg               <  1:196709/148‑1 (MQ=255)
                                                                                                                                 aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt        >  5:60109/1‑149 (MQ=255)
                                                                                                                                  aagaagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtttt       >  3:214342/7‑149 (MQ=255)
                                                                                                                                     aagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt      <  2:31779/144‑1 (MQ=255)
                                                                                                                                     aagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTc     >  3:349011/4‑148 (MQ=255)
                                                                                                                                     aagaaCGCCCGGATATTTCCCGGGCGTTCTACTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt      >  1:31779/4‑147 (MQ=255)
                                                                                                                                       gaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt  >  7:32667/2‑149 (MQ=255)
                                                                                                                                        aaCGCCCTGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt  <  4:37544/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
GCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAT  >  NC_002947/3300709‑3300992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑